Immune-cell-derived membranes have garnered significant attention as innovative delivery modalities in cancer immunotherapy for their intrinsic immune-modulating functionalities and superior biocompatibilities. Integrating additional parental cell or synthetic lipid vesicles into cellular can further potentiate capacities to perform combinatorial pharmacological activities activating antitumor immunity, thus providing insights the potential of hybrid versatile vehicles immunotherapy. Here,...

Abstract We describe a small lipid nanoparticle (SLNP)‐based nanovaccine platform and new combination treatment regimen. Tumor antigen‐displaying, CpG adjuvant‐embedded SLNPs (OVA PEP ‐SLNP@CpG) were prepared from biocompatible phospholipids cationic cholesterol derivative. The resulting showed highly potent antitumor efficacy in both prophylactic therapeutic E.G7 tumor models. However, this vaccine induced T cell exhaustion by elevating PD‐L1 expression, leading to recurrence. Thus, the was...

Thermoplastic blends are applied for three-dimensional (3D) printing to obtain improved functionality. While thermal, chemical, and mechanical properties of 3D-printed typically examined, biodegradability the plastics has rarely been focus research. In this study, we evaluated biodegradation behavior prototypes fabricated from various blends, including biodegradable polylactic acid (PLA), poly(3-hydroxybutyrate) (PHB), non-biodegradable high-density polyethylene (HDPE), polypropylene (PP)....

Cancer-associated fibroblasts (CAFs), which are dominant cell types in the tumor microenvironment (TME), support growth by secreting cytokines and forming an extracellular matrix (ECM) that hampers penetration of chemical biological therapeutics within thereby limits their therapeutic efficacy. Here, we report a cancer nanovaccine targeting fibroblast activation protein α (FAP)-expressing CAFs as potential pan-tumor vaccine. We predicted immunodominant FAP-specific epitope peptides silico...

Organ-selective drug delivery is expected to maximize the efficacy of various therapeutic modalities while minimizing their systemic toxicity. Lipid nanoparticles and polymersomes can direct organ-selective mRNAs or gene editing machineries, but limited mostly liver, spleen, lung. A platform that enables these other target organs urgently needed. Here, a library glycocalyx-mimicking (GlyNPs) comprising five randomly combined sugar moieties generated, in vivo screening used identify GlyNPs...

Ischemia-reperfusion injury (IRI) poses significant challenges across various organ systems, including the heart, brain, and kidneys. Exosomes have shown great potentials applications in mitigating IRI-induced cell tissue damage through modulating inflammatory responses, enhancing angiogenesis, promoting repair. Despite these advances, a more systematic understanding of exosomes from different sources their biotransport is critical for optimizing therapeutic efficacy accelerating clinical...

This retrospective cohort study included all user of tumor necrosis factor-α inhibitors (TNFi), including etanercept, infliximab, adalimumab, and golimumab, among Korean patients with psoriasis rheumatoid arthritis (N = 7,645) the non-user who was diagnosed same diseases, never used TNFi, served as reference. Cumulative usage TNFi calculated between newly date index (2-year from date). Adjusted hazard ratio (aHR [95% confidence intervals (CIs)]) for colorectal, liver, lung, kidney, breast,...

This study was aimed at investigating the functional significance of heparan sulfate (glucosamine) 3-O-sulfotransferase 2 (HS3ST2) hypermethylation in non-small cell lung cancer (NSCLC).HS3ST2 characterized six lines, and its clinical analyzed using 298 formalin-fixed paraffin-embedded tissues 26 fresh-frozen from 324 NSCLC patients. MS-HRM (methylation-specific high-resolution melting) EpiTYPER(TM) assays showed substantial CpG island promoter region HS3ST2 lines. The silenced gene...

This study was aimed at understanding the clinicopathological significance of HOXA9 hypermethylation in non‐small cell lung cancer (NSCLC). characterized six lines, and its analyzed using methylation‐specific PCR 271 formalin‐fixed paraffin‐embedded tissues 27 fresh‐frozen tumor matched normal from 298 NSCLC patients, Ki‐67 expression immunohistochemistry. The promoter region highly methylated but not bronchial epithelial cells. loss restored by treatment cells with a demethylating agent,...

// Dong Hao Jin 1 , Yujin Kim Bo Bin Lee Joungho Han 2 Hong Kwan 3 Young Mog Shim and Duk-Hwan Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School Medicine, Suwon, 440-746, Korea Pathology, Medical Center, Seoul, 135-710, Thoracic Cardiovascular Surgery, Correspondence to: Kim, email: dukhwan.kim@samsung.com Keywords: metformin, lung cancer, cell cycle, E2F8, p21 Received: June 29, 2017      Accepted: September...

Patients with BRAFV600E-mutant melanoma are effectively treated the BRAF-inhibiting drug, vemurafenib, but soon develop drug resistance, limiting vemurafenib's therapeutic efficacy. Constitutive activation of STAT3 in cancer cells and immune tumor microenvironment (TME) is a crucial contributor to development resistance evasion most cancers. Here, we investigated antitumor efficacy TME remodeling by APTSTAT3-9R, cell-permeable inhibitory peptide, as strategy treat vemurafenib-resistant...

Abstract Tumor‐associated macrophages (TAMs)—representative immune‐suppressive cells in the tumor microenvironment (TME)—are known to promote progression and metastasis, thus are considered an attractive target for cancer therapy. However, current TAM‐targeting strategies insufficient result robust antitumor efficacy. Here, a small lipid nanoparticle encapsulating immunostimulatory CpG oligodeoxynucleotides (SLNP@CpG) is reported as new immunotherapeutic modality that can reprogram TAMs...

Abstract Background Recently, various studies have revealed that 3D cell spheroids several advantages over 2D cells in stem culture. However, conventional spheroid culture methods some disadvantages and limitations such as time required for formation complexity of the experimental process. Here, we used acoustic levitation platform to overcome limitation methods. Methods In our anti-gravity bioreactor, continuous standing sonic waves created pressure field human mesenchymal (hMSCs). hMSCs...

Abstract Background Human adipose-derived stem cells (hADSCs) have been widely used for regenerative medicine because of their therapeutic efficacy and differentiation capacity. However, there are still limitations to use them intactly due some difficulties such as poor cell engraftment viability after transplantation. Therefore, techniques photobiomodulation (PBM) required overcome these limitations. This study probed improved preclinical irradiated hADSCs its underlying molecular...

Mitochondria are organelles that play a vital role in cellular survival by supplying ATP and metabolic substrates via oxidative phosphorylation the Krebs cycle. Hence, mitochondrial dysfunction contributes to many human diseases, including syndromes, neurodegenerative cancer, aging. Mitochondrial transfer between cells has been shown occur naturally, transplantation is beneficial for treating dysfunction. In this study, migration of mitochondria was tracked vitro vivo using conjugated with...

Abstract Background To understand the molecular mechanisms involved in regulation of DNA methyltransferases (DNMTs) by metformin non-small cell lung cancer (NSCLC) cells. Methods Expression levels DNMTs response to were analyzed NSCLC MicroRNAs regulating expression at post-transcriptional level searched using miRNA-target databases (miRDB and miRTarBase), TCGA RNASeqV2 data, miRNA-seq. Results Metformin dose-dependently downregulated DNMT1 DNMT3a DNMT3b transcriptional A549 Activity was...

Abstract BACKGROUND: The objective of this was to identify functional single nucleotide polymorphisms (SNPs) in cyclin‐dependent kinases (CDKs) and cyclins that are associated with risk human cancer. METHODS: First, 45 SNPs CDKs were analyzed 106 lung cancers 108 controls for a pilot study. One SNP (reference [rs] 769236, +1 guanine adenine [G→A]) at the promoter region cyclin A2 ( CCNA2 ) also 1989 (300 breast cancers, 450 colorectal gastric 367 hepatocellular carcinomas, 422 cancers) 1096...

This study was aimed at understanding the functional significance of HOXA11 hypermethylation in non-small cell lung cancer (NSCLC). characterized six lines, and its clinical analyzed using formalin-fixed paraffin-embedded tissues from 317 NSCLC patients, Ki-67 expression immunohistochemistry. The promoter region highly methylated but not normal bronchial epithelial cells. loss restored by treatment cells with a demethylating agent, 5-aza-2'-deoxycytidine (5-Aza-dC). Transient transfection...