A5007024407- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Cancer-related gene regulation
- Lung Cancer Treatments and Mutations
- Cancer Genomics and Diagnostics
- Cancer-related molecular mechanisms research
- Cancer Cells and Metastasis
- Chemical Reactions and Isotopes
- Genetic factors in colorectal cancer
- DNA and Nucleic Acid Chemistry
- Cancer Research and Treatments
- Cancer, Lipids, and Metabolism
- Cancer-related Molecular Pathways
- Ubiquitin and proteasome pathways
- Agriculture, Soil, Plant Science
- Lung Cancer Research Studies
- Sarcoma Diagnosis and Treatment
- Food Quality and Safety Studies
- Medical Imaging and Pathology Studies
- Mass Spectrometry Techniques and Applications
- Genetic Associations and Epidemiology
- Amino Acid Enzymes and Metabolism
- Biopolymer Synthesis and Applications
- Ecology and Conservation Studies
- Radiopharmaceutical Chemistry and Applications
Sungkyunkwan University
2014-2024
Samsung (South Korea)
2008-2023
Samsung Medical Center
2003-2018
Kyungpook National University
2011-2015
National Academy of Agricultural Science
2014
Pusan National University
2010
Biomedical Research Institute
2004-2006
Samsung (United Kingdom)
2006
Beth Israel Deaconess Medical Center
2001
Harvard University
2001
Abstract Background Silicon (Si) application has been known to enhance the tolerance of plants against abiotic stresses. However, protective mechanism Si under heavy metals contamination is poorly understood. The aim this study was assess role in counteracting toxicity due cadmium (Cd) and copper (Cu) rice ( Oryza sativa ). Results significantly improved growth biomass reduced toxic effects Cd/Cu after different stress periods. treatment ameliorated root function structure compared with...
Abstract Purpose: To identify epigenetic molecular makers in plasma for the early detection of colorectal cancer. Experimental Design: We retrospectively analyzed methylation status 10 genes fresh-frozen tissues and corresponding samples from 243 patients with stage I II sporadic cancer, 276 healthy individuals, 64 adenoma using methylation-specific PCR. The score (Mscore) was used to find markers high sensitivity specificity. Results: Of cancer tissues, detected 18% p14, 34% p16, 27% APC,...
Metastatic relapse of primary lung cancer leads to therapeutic resistance and unfavorable clinical prognosis; therefore, identification key molecules associated with metastatic conversion has significant implications. We previously identified a link between early brain metastasis adenocarcinoma amplification the α-smooth muscle actin (ACTA2) gene. The aim present study was investigate prognostic functional significance ACTA2 expression in cells for potential adenocarcinomas.ACTA2 analyzed...
Purpose The aim of this study was to identify tumor-specific methylation in bronchial lavage for the early detection non-small-cell lung cancer (NSCLC) by differentiating age-related from NSCLC. Patients and Methods Eighty-five NSCLC patients 127 cancer-free subjects participated study. Aberrant at promoters p16, Ras association domain family 1A (RASSF1A), fragile histidine triad (FHIT), H-cadherin, retinoic acid receptor β (RARβ) genes were evaluated resected tumor tissues samples...
Deamination of nucleotides causes C:G>T:A changes in formalin-fixed, paraffin-embedded (FFPE) tissue samples and produces false positives during next-generation sequencing (NGS). Uracil DNA glycosylase (UDG) helps eliminate this issue, but the effect UDG different preparation conditions has not been rigorously studied. To investigate whether can reduce false-positive single-nucleotide variant (SNV) calls, we used tumor normal tissues from gastric adenocarcinoma patients prepared using...
TNF receptor-associated factor 6 (TRAF6)-BECN1 signaling axis plays a pivotal role in autophagy induction through ubiquitination of BECN1, thereby inducing lung cancer migration and invasion response to toll-like receptor 4 (TLR4) stimulation. Herein, we provide novel molecular cellular mechanisms involved the negative effect ubiquitin-specific peptidase 15 (USP15) on progression. Clinical data TCGA primary non-small cell (NSCLC) patients (n = 41) revealed that expression USP15 was...
Abstract Background Free fatty acid receptors (FFARs) and toll-like (TLRs) recognize microbial metabolites conserved products, respectively, are functionally implicated in inflammation cancer. However, whether the crosstalk between FFARs TLRs affects lung cancer progression has never been addressed. Methods We analyzed association using The Cancer Genome Atlas (TCGA) data our cohort of non-small cell (NSCLC) patient (n = 42), gene set enrichment analysis (GSEA) was performed. For functional...
// Su Jin Hwang 1, * , Hye Won Lee 2, Ree Kim 1 Song 4 Dong Heon 3 Hong Chang Hoon Shin Je-Gun Joung 5 Duk-Hwan 4, 6 Kyeung Min Joo Hyeon Ho 7 Department of Health Sciences and Technology, Samsung Advanced Institute for Sungkyunkwan University, Seoul, Korea 2 Urology, Medical Center, University School Medicine, Neurosurgery, Refractory Cancer Research, Anatomy Cell Biology, Suwon, Translational Bioinformatics Laboratory, Genome Institute, Center Biomedical Research Future These authors have...
Abstract β‐arrestin 2 (ARRB2) is functionally implicated in cancer progression via various signaling pathways. However, its role lung remains unclear. To obtain clinical insight on function cancer, microarray data from tumor tissues (LTTs) and matched normal (mLNTs) of primary non-small cell (NSCLC) patients ( n = 37) were utilized. ARRB2 expression levels markedly decreased all 37 LTTs compared to those LNTs NSCLC patients. They significantly co-related enrichment gene sets associated with...
Abstract Despite advances in the detection and treatment of lung cancer, prognosis for patients with cancer is poor, partly as a result recurrences. We retrospectively analyzed relationship between recurrence survival non–small cell cancers (NSCLC), promoter methylation p16, GSTP1, FHIT, H-cadherin, RARβ2 genes to identify prognostic molecular marker associated NSCLC. Methylation status from 335 paraffin blocks was determined by methylation-specific PCR. Of NSCLC samples, detected 35% 39%...
This study was aimed at investigating the functional significance of heparan sulfate (glucosamine) 3-O-sulfotransferase 2 (HS3ST2) hypermethylation in non-small cell lung cancer (NSCLC).HS3ST2 characterized six lines, and its clinical analyzed using 298 formalin-fixed paraffin-embedded tissues 26 fresh-frozen from 324 NSCLC patients. MS-HRM (methylation-specific high-resolution melting) EpiTYPER(TM) assays showed substantial CpG island promoter region HS3ST2 lines. The silenced gene...
Abstract Brain metastasis is the most common type of intracranial cancer and main cause cancer-associated mortality. mainly originates from lung cancer. Using a previously established in vitro brain metastatic model, we found that PC14PE6/LvBr4 cells exhibited higher expression β-catenin increased migratory activity than parental PC14PE6 cells. Knockdown dramatically suppressed motility invasiveness cells, indicating involved controlling potential. Since protein was without significant...
// Dong Hao Jin 1 , Yujin Kim Bo Bin Lee Joungho Han 2 Hong Kwan 3 Young Mog Shim and Duk-Hwan Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School Medicine, Suwon, 440-746, Korea Pathology, Medical Center, Seoul, 135-710, Thoracic Cardiovascular Surgery, Correspondence to: Kim, email: dukhwan.kim@samsung.com Keywords: metformin, lung cancer, cell cycle, E2F8, p21 Received: June 29, 2017 Accepted: September...
In the era of targeted therapy, mutation profiling cancer is a crucial aspect making therapeutic decisions. To characterize at molecular level, use formalin-fixed paraffin-embedded tissue important. We tested Ion AmpliSeq Cancer Hotspot Panel v2 and nCounter Copy Number Variation Assay in 89 gastric samples to determine whether they are applicable archival clinical for personalized therapies. validated results with Sanger sequencing, real-time quantitative PCR, fluorescence situ...
// Dong-Hao Jin 1 , Jeeyun Lee 2 Kyoung Mee Kim 3 Sung 4 Duk-Hwan and Joobae Park Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School Medicine, Suwon, Korea Internal Medical Center, Seoul, Pathology Translational Genomics, Surgery, Correspondence to: Kim, email: Park, Keywords : gastric cancer; copy number alteration; MAPK15; c-Jun; stability Received December 01, 2014 Accepted May 02, 2015 Published 19, Abstract This study was aimed at...
Abstract Background To understand the molecular mechanisms involved in regulation of DNA methyltransferases (DNMTs) by metformin non-small cell lung cancer (NSCLC) cells. Methods Expression levels DNMTs response to were analyzed NSCLC MicroRNAs regulating expression at post-transcriptional level searched using miRNA-target databases (miRDB and miRTarBase), TCGA RNASeqV2 data, miRNA-seq. Results Metformin dose-dependently downregulated DNMT1 DNMT3a DNMT3b transcriptional A549 Activity was...
This study was aimed at understanding the functional significance of HOXA11 hypermethylation in non-small cell lung cancer (NSCLC). characterized six lines, and its clinical analyzed using formalin-fixed paraffin-embedded tissues from 317 NSCLC patients, Ki-67 expression immunohistochemistry. The promoter region highly methylated but not normal bronchial epithelial cells. loss restored by treatment cells with a demethylating agent, 5-aza-2'-deoxycytidine (5-Aza-dC). Transient transfection...