A5053985242- Organ and Tissue Transplantation Research
- Transplantation: Methods and Outcomes
- Organ Donation and Transplantation
- Reconstructive Surgery and Microvascular Techniques
- Sarcoma Diagnosis and Treatment
- Bone Tumor Diagnosis and Treatments
- Head and Neck Cancer Studies
- Antibiotics Pharmacokinetics and Efficacy
- Scoliosis diagnosis and treatment
- Connective tissue disorders research
- Hepatitis Viruses Studies and Epidemiology
- Genetic Syndromes and Imprinting
- Heterotopic Ossification and Related Conditions
- Genetic and Kidney Cyst Diseases
- Ear and Head Tumors
- Lipoproteins and Cardiovascular Health
- Cardiac Ischemia and Reperfusion
- Myasthenia Gravis and Thymoma
- Renal and related cancers
- Coccidia and coccidiosis research
- Escherichia coli research studies
- Gallbladder and Bile Duct Disorders
- Cervical and Thoracic Myelopathy
- Renal Transplantation Outcomes and Treatments
- Head and Neck Surgical Oncology
Shiga University of Medical Science
2011-2025
National Defense Medical College
2016-2024
Kobe University Hospital
2009
Kobe University
2008-2009
Hokkaido University
1993
Abstract Autosomal dominant polycystic kidney disease (ADPKD) caused by PKD1 mutations is one of the most common hereditary disorders. However, key pathological processes underlying cyst development and exacerbation in pre-symptomatic stages remain unknown, because rodent models do not recapitulate critical phenotypes, including onset heterozygotes. Here, using CRISPR/Cas9, we generate ADPKD with cynomolgus monkeys. As humans mice, near-complete depletion induces severe formation mainly...
Abstract Non-human primates, such as cynomolgus monkeys, are invaluable experimental models for understanding human biology and disease. Their close genetic relationship to humans makes them essential studying fundamental developmental processes disease progression. Although lentiviral methods generating transgenic monkeys exist, several inherent technical difficulties limit their utility. To solve this problem, here we establish a non-viral method using the piggyBac transposon system. After...
The objective of this study was to examine the allowable warm ischemic time and pathological changes due ischemia reperfusion injury in uterus cynomolgus monkey as a model for transplantation.Six female monkeys were used study. resected from vaginal canal connected through bilateral ovarian uterine arteries veins only. One animal control. In other five animals, vessels clamped 0.5, 1, 2, 4 8 h, respectively. Biopsy smooth muscle tissue corpus uteri performed after each subsequent 3 h....
How long is the allowable warm ischemic time of uterus and what morphological biochemical changes are caused by uterine ischemia/reperfusion injury in cynomolgus macaques?Warm ischemia macaques tolerated for up to 4 h reperfusion after no further changes.Uterus transplantation a potential option women with factor infertility. The humans non-human primates unknown.This experimental study included 18 female periodic menstruation.Animals were divided into six groups three monkeys each: control...
Abstract No study has reported an animal model of uterus transplantation (UTx) using cynomolgus macaques. We aimed to establish a surgical technique allogeneic UTx assuming the recovery from deceased donor in Four UTxs were performed female Donor surgeries comprised en bloc organs with iliac vessels on both sides, and/or abdominal aorta/vena cava after sufficient perfusion one femoral artery or external artery. Before perfusion, 150 mL whole blood was obtained for subsequent transfusion...
Nodular hidradenoma (NH) is a benign adnexal tumor that arises from either eccrine or apocrine sweat glands. NH can originate any cutaneous site, but the most common sites are head and anterior surface of trunk, with very rare cases in extremities. Long-standing has been reported to undergo malignant transformation (MNH); however, its occurrence lower leg extremely only one other case date. In this report, we present MNH occurring which was resected intent make diagnosis. At final follow-up...
Uterus transplantation (UTx) is an option for women with uterine factor infertility to have a child, but still in the experimental stage. Therefore, allogeneic animal models of UTx are required resolution clinical issues. In this study, long-term outcomes were evaluated four recipients (cases 1-4) after cynomolgus macaques. Immunosuppression antithymocyte globulin induction and triple maintenance regimen was used. Postoperative ultrasonography biopsy transplanted uterus immunoserological...
Abstract Uterus transplantation (UTx) is a potential option for women with uterine factor infertility to have child. The clinical features indicating irreversible rejection of the uterus are unknown. In our experimental series allogeneic UTx in cynomolgus macaques, six female macaques were retrospectively examined, which unresponsive treatment immunosuppressants (i.e. rejection). Clinical including general condition, hematology, size, indocyanine green (ICG) fluorescence imaging by...
Delivery following uterus transplantation (UTx)—an approach for treating uterine factor infertility—has not been reported in nonhuman primate models. Here, six female major histocompatibility complex (MHC)-defined cynomolgus macaques that underwent allogeneic UTx were evaluated. Antithymocyte globulin and rituximab administered to induce immunosuppression a triple maintenance regimen was used. Menstruation resumed all animals with long-term survival, except one, which euthanized due infusion...
The results presented here provide evidence of the enhancing effect oral fluoropyrimidine derivative S-1 in concomitant chemoradiotherapy for head and neck cancer further insights into its biological mechanism.To investigate additive radiation human hypopharyngeal cancer.Nude mice bearing cells (H891) were used an vivo model. was administered at a volume 0.01 mg/g body weight per mouse 14 days, tumors irradiated with 2.0 Gy on days 1 8. Mice treated either or alone as controls. growth each...
A serological survey of hepatitis E virus (HEV) antibody was conducted using 202 adult captive cynomolgus monkeys, who did not show any clinical signs acute hepatitis. Out these, 44 monkeys were sero-positive for anti-HEV IgG and all negative IgM. All positive came from either Vietnam or China, but none the Philippines, Indonesia, our facility. Selected 12 out Vietnum, including 9 3 negative, revealed mostly within reference ranges alanine aminotransferase (ALT) asparatate (AST) by serum...
<h3>Objective</h3> To explore the potential clinical application of oncolytic activity cyclooxygenase 2 (COX-2) promoter–based, conditional, replication-selective adenovirus vector for hypopharyngeal squamous cell carcinoma. <h3>Design</h3> In vivo study and retrospective study. <h3>Setting</h3> Kobe University Hospital, Kobe, Japan. <h3>Subjects</h3> Expression COX-2 in cancers treated at Hospital was immunohistochemically investigated. addition, nude mice bearing human cancer cells (H891)...
Familial hypercholesterolemia (FH) is an inherited autosomal dominant disorder that associated with a high plasma level of low-density lipoprotein (LDL) cholesterol, leading to increased risk cardiovascular diseases. To develop basic and translational research on FH, we here generated FH model in non-human primate (cynomolgus monkeys) by deleting the LDL receptor (LDLR) gene using genome editing technique. Six LDLR knockout (KO) monkeys were produced, all which confirmed have mutations...
There are few effective antimicrobial agents against Balantidium coli infection. The effect of paromomycin sulfate (PS) B. was confirmed in this study 596 captive cynomolgus monkeys. In several trials, the minimum dose and duration oral administration PS were 25 mg/day for 5 + days, with a 2-day withdrawal interval. To facilitate daily administration, pumpkin cakes supplemented made, which not only resulted precise effects but also increased efficiency preparation by animal care staff. No...
Traumatic heterotopic ossification is a condition in which extraskeletal bone formation occurs soft tissues after injury. It most commonly patients who had major orthopedic surgery and those with severe extremity injuries. The lesion causes local pain can impair motor function of the affected limb, but there currently no established prophylaxis or treatment for this condition. In study, we show that immobilization at an early stage inflammatory response injury attenuate murine Achilles...
The LBX1 gene is located near a single nucleotide polymorphism that highly associated with susceptibility to adolescent idiopathic scoliosis and considered one of the strongest candidate genes involved in pathogenesis this condition. We have previously found loss from skeletal muscle results not only spinal deformity but also lean body mass, suggesting potential role for energy metabolism. purpose present study was test hypothesis by analyzing phenotype mice lacking focus on rendered more...
Abstract Heterotopic ossification is abnormal bone formation in soft tissues that occurs primarily after injury and major surgery. This condition often causes local pain limits joint motion the affected limb. Currently, there no effective treatment or prophylaxis for this other than surgical removal of lesion. Recent studies suggest retinoic acid receptor (RAR) agonists are suppressing heterotopic patients with Fibrodysplasia Ossificans Progressiva, a congenital disorder characterized by...
Over the past few decades, clinical outcomes of patients with cancer have significantly improved mostly owing to development effective chemotherapeutic treatments. However, chronic health conditions such as bone mass loss and risk fragility fractures caused by chemotherapy also emerged crucial issues in treated for cancer. In this study, we aimed understand effect eribulin mesylate (ERI), a microtubule-targeting agent currently used treat metastatic breast certain subtypes advanced sarcomas,...
Objects: The aim of this study was to evaluate the clinical features and salvage surgery after radiotherapy in patients with early glottic cancer. Patients Methods: From 2001 2006, 88 T1 T2 cancer were treated radiation alone (82 patients)and chemoradiation(6 patients). In general, cancers bulky tumors without deep invasion hyperfractionated (19 platinum-based concurrent chemoradiation. Local failure occurred 14 (rest 4 recurrence 10 Median follow-up 31 months. Results: control rates 90％,...
Abstract LBX1 is a gene located near single‐nucleotide polymorphism, rs11190870, which highly associated with susceptibility to adolescent idiopathic scoliosis. However, the potential involvement of in etiology this spinal deformity has not been elucidated. In study, we aimed determine whether lack skeletal muscle results deformities mice. We generated mutant mice Lbx1 allele was conditionally excised under control human actin promoter. Mice lacking from were fertile and available. The had...
Objective : To evaluate the treatment results of patients with early glottic cancer respect to laryngeal preservasion.Patients and Methods Between 2001 2005, 93 were treated at Kobe University Hospital. In general, T1 T2 cancers conventional hyperfractionated radiotherapy, respectively. Conservative surgery preservation was considered as salvage for radiation failure.Results The larynx preserved in 95.8% (45/47) 82.6% (19/23).Conclusion Although follow up periods not long enough, promising...