A5088236070- Cancer, Hypoxia, and Metabolism
- Single-cell and spatial transcriptomics
- RNA modifications and cancer
- Endometriosis Research and Treatment
- Drug-Induced Hepatotoxicity and Protection
- Ovarian cancer diagnosis and treatment
- Advanced Proteomics Techniques and Applications
- Olfactory and Sensory Function Studies
- Nanoparticle-Based Drug Delivery
- Bioactive Compounds and Antitumor Agents
- Chemical Reactions and Isotopes
- Cancer Research and Treatments
- Drug Transport and Resistance Mechanisms
- Pharmacogenetics and Drug Metabolism
- interferon and immune responses
- Cancer Genomics and Diagnostics
- Neuroinflammation and Neurodegeneration Mechanisms
- Reproductive System and Pregnancy
- Microbial Natural Products and Biosynthesis
- 3D Printing in Biomedical Research
- MicroRNA in disease regulation
- Synthesis and Biological Evaluation
- Graphene and Nanomaterials Applications
- Cancer Cells and Metastasis
- Epigenetics and DNA Methylation
Dr. Margarete Fischer-Bosch-Institute of Clinical Pharmacology
2023-2025
University of Tübingen
2023-2025
Novartis Institutes for BioMedical Research
2018
Novartis (Switzerland)
2018
Ludwig-Maximilians-Universität München
2007
Leipzig University
1987
Background Pancreatic ductal adenocarcinoma (PDAC) is a highly lethal cancer with 5-year survival rate of 12%. It has two major molecular subtypes: classical and basal, regulated by the master transcription factors (MTFs) GATA6 ΔNp63, respectively. Objective This study sought to uncover transcriptional regulatory mechanisms controlling PDAC subtype identity. Design We integrated primary tumour single-cell RNA-seq, patient-derived xenograft RNA-seq multispectral imaging identify...
ABSTRACT Type III interferons (IFNs) primarily act on epithelial cells and protect against virus infection of the mucosa, whereas type I IFNs more systemically. To date, it has been unknown which subtypes in upper airways, primary site for initial most respiratory viruses, rely IFN or antiviral protection. address this question, we performed a single-cell transcriptomics analysis IFN-mediated response focusing airways mice. This work identified nine distinct cell types derived from olfactory...
Abstract Immunotherapies have emerged as a promising pillar for cancer therapy. However, due to their reliance on the individual, complex and dynamic tumor microenvironment, treatment success remains limited subset of patients. A deeper understanding response individual tumors heterogeneous microenvironment is essential improve patient stratification develop novel approaches. This requires suitable model systems which allow tracking microenvironment‘s (TME) immunotherapy. In this study, we...
Abstract Introduction: Breast cancer is one of the most common and deadly cancers among women, highlighting need for reliable in vitro models to study tumor biology assess therapeutic strategies. In this project, we established characterized three breast tumoroid lines (two triple negative HER2+), derived from patient-derived xenograft as a robust system research: MAXFHER 2500, MAXFT 583 MAXFTN 2988. The tumoroids were maintained under various conditions, including long-term culture,...
Abstract Gastrointestinal stromal tumors (GIST) are the most prevalent mesenchymal tumor entity of gastrointestinal tract and serve as a key example for targeted therapies with tyrosine kinase inhibitors (TKI) such imatinib, in frequent mutations PDGFRA or c-Kit. Although TKIs have significantly improved overall survival rates GIST patients, there currently no treatment options imatinib-resistant without identifiable driver mutations. So far, current sarcoma research mostly relies on...
Abstract Lung cancer remained the most frequently diagnosed in 2022 and leading cause of cancer-related deaths worldwide. Oncology drug development attrition is highest compared with other therapeutic areas, success rates clinical range from 3.4-5%. Tumoroids as sophisticated three-dimensional vitro cultures serve an advanced near-physiological model but lung tumoroid remains challenging. The treatment strategy for patients currently determined based on histology, stage, biomarker status,...
Abstract High-grade serous ovarian cancer (HGSOC) represents the most common and lethal subtype of cancer. Despite initial response to platinum-based standard therapy, patients commonly suffer from relapse that likely originates drug-tolerant persister (DTP) cells. We generated isogenic clones treatment-naïve cisplatin-tolerant HGSOC In addition, single-cell RNA sequencing barcoded cells was performed in a xenograft model with cell lines after therapy. Published RNA-sequencing data...
Precision-cut tumor slices (PCTS) maintain tissue heterogeneity concerning different cell types and preserve the microenvironment (TME). Typically, PCTS are cultured statically on a filter support at an air–liquid interface, which gives rise to intra-slice gradients during culture. To overcome this problem, we developed perfusion air culture (PAC) system that can provide continuous controlled oxygen medium, drug supply. This makes it adaptable ex vivo for evaluating responses in...
Abstract Ovarian cancer is a complex and heterogeneous disease the major cause of death among women with gynecological cancers. While patients usually respond well to platinum-based first-line chemotherapy, often becomes increasingly resistant treatment. The tumor microenvironment (TME) plays an important role in development drug resistance. Characterizing TME ovarian after treatment essential identify molecular mechanisms that allow residual cells survive chemotherapy cancer. We have...
Low-grade endometrial stromal sarcoma (LG-ESS) is a rare uterine malignancy characterized by its complex tumor microenvironment (TME) and high recurrence rates, posing challenges to accurate prognosis effective treatment. Identifying prognostic biomarkers essential for improving patient stratification guiding therapeutic strategies.
Abstract Niphathesine C and related pyridine alkaloids are well known natural products with interesting antimicrobial activities, characterized by a ring lipophilic side chain terminal nitrogen‐containing functional group. This paper describes the synthesis of analogues these alkylpyridine variation heterocyclic Key steps syntheses Sonogashira reaction appropriate aryl iodides undec‐10‐ynol or undec‐10‐ynoic acid derivatives. The resulting compounds were tested in an agar diffusion assay...
Abstract The complex and dynamic microenvironment of tumors influences their development, progression, response to therapy. In addition cancer cells, solid consist a tumor (TME) containing fibroblasts, immune blood lymphatic vessels, the extracellular matrix. A wide variety secreted proteins maintain heterotypic interactions between cell types in TME. However, it is not well understood how TME responds treatment at proteome level. Here, we developed unique nascent proteomic approach for...
1. The utility of 1-aminobenzotriazole (ABT), incorporated in food, has been investigated as an approach for longer term inhibition cytochrome P450 (P450) enzymes mice.2. In rats, ABT inhibits gastric emptying, to investigate this potential limitation mice we examined the effect administration on oral absorption NVS-CRF38. Two hour prior treatment with 100 mg/kg inhibited NVS-CRF38, Tmax was 4 hours ABT-treated compared 0.5 control group.3. A marked hepatic activity observed fed containing...