A5012170714- RNA modifications and cancer
- Cancer-related gene regulation
- Genetics and Neurodevelopmental Disorders
- Cellular transport and secretion
- Neuroscience and Neuropharmacology Research
- Signaling Pathways in Disease
- Epigenetics and DNA Methylation
- Protein Kinase Regulation and GTPase Signaling
- RNA Research and Splicing
- Acute Myeloid Leukemia Research
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Biochemical and Molecular Research
- Adenosine and Purinergic Signaling
- Biotechnology and Related Fields
- DNA and Nucleic Acid Chemistry
- Fatty Acid Research and Health
- Genetic Associations and Epidemiology
- Genetics, Bioinformatics, and Biomedical Research
- RNA and protein synthesis mechanisms
- Parvovirus B19 Infection Studies
- Enzyme Structure and Function
- Antioxidant Activity and Oxidative Stress
- Chronic Myeloid Leukemia Treatments
- Genomics and Chromatin Dynamics
- Animal Genetics and Reproduction
Chengdu University of Traditional Chinese Medicine
2024
Millennium Engineering and Integration (United States)
2023
Boston Consulting Group (United States)
2023
Zhongshan Hospital of Xiamen University
2019
Cornell University
2011-2014
College of Staten Island
2012
City University of New York
2006-2012
New York State Office for People With Developmental Disabilities
2006-2012
University of Pittsburgh
2004
Duke University Hospital
1994-1997
Different domains of the serine/threonine kinase, raf-1, were expressed as fusion proteins with glutathione S-transferase (GST) in Escherichia coli and purified to near homogeneity by affinity chromatography. A cysteine-rich domain raf-1 was found contain 2 mol zinc (molar basis), similar analogous protein kinase C. GST-fusion proteins, containing bound liposomes a phosphatidylserine-dependent manner. In contrast C, translocation not dependent upon diacylglycerol, phorbol ester, or calcium,...
Cancer cells metabolize different energy sources to generate biomass rapidly. The purine biosynthetic pathway was recently identified as an important source of metabolic intermediates for these processes. However, very little known about the regulatory mechanisms metabolism in hepatocellular carcinoma (HCC). We explored role dual‐specificity tyrosine (Y) phosphorylation–regulated kinase 3 (Dyrk3) HCC metabolism. Dyrk3 significantly down‐regulated compared with normal controls. Its...
Minibrain kinase/dual-specificity tyrosine phosphorylation-regulated kinase (Mnb/Dyrk1A) is a proline-directed serine/threonine encoded in the Down syndrome critical region of human chromosome 21. This has been shown to phosphorylate dynamin 1 and synaptojanin 1. Here we report that amphiphysin I (Amph I) also Mnb/Dyrk1A substrate. phosphorylated native Amph rodent brains recombinant expressed Escherichia coli. Serine 293 (Ser-293) was identified as major site, whereas serine 295 threonine...
Lipid oxidation is commonly seen in the innate immune response, which reactive oxygen intermediates are generated to kill pathogenic microorganisms. Although products of phospholipids have generally been regarded play a role number chronic inflammatory processes, several studies shown that oxidized inhibit LPS-induced acute proinflammatory response cultured macrophages and endothelial cells. We report this study 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphorylcholine (PAPC), but not...
Dyrk1A phosphorylated multiple proteins in the clathrin-coated vesicle (CCV) preparations obtained from rat brains. Mass spectrometric analysis identified MAP1A, MAP2, AP180, and α- β-adaptins as CCVs. Each protein was subsequently confirmed by [(32)P]-labeling immunological methods. The Dyrk1A-mediated phosphorylation released majority of MAP1A MAP2 enhanced release AP180 adaptin subunits Furthermore, displaced adaptor physically CCVs a kinase-concentration dependent manner. clathrin heavy...
Stress granules contain a large number of post-translationally modified proteins, and studies have shown that these modifications serve as recruitment tags for specific proteins even control the assembly disassembly themselves. Work originating from our laboratory has focused on role protein methylation plays in stress granule composition function. We demonstrated both asymmetrically symmetrically dimethylated are core constituents granules, we endeavored to understand when how this occurs....
Objective Previous studies have shown that emotional disorders are negatively associated with heart rate variability (HRV), but the potential causal relationship between genetic susceptibility to and HRV remains unclear. We aimed perform a Mendelian randomization (MR) study investigate association HRV. Methods The data used for this were obtained from publicly available genome-wide datasets. Five models, including inverse variance weighted model (IVW), median estimation (WME), model-based...
Dynamin 1 is thought to mediate synaptic transmission through interactions with multiple endocytic accessory proteins in a phosphorylation-dependent manner. Previously, we have shown that DYRK1A, chromosome 21-encoded kinase implicated the mental retardation of Down syndrome, phosphorylates primarily serine 857 (S857) proline-rich domain, found only 1xa, one alternative C-terminal splicing isoforms dynamin 1. 1xa and 1xb are able assemble into heterologous complexes coregulated by DYRK1A...
Protein arginine methyltransferases (PRMTs) function in the alkaline milieu of nucleus and at neutral pH cytosol. Accordingly, several PRMTs are broadly active over a range pHs. We investigated effect altering had on protein methylation using variety defined substrates, recombinant cell extracts.We demonstrate that pH-induced alterations extent methyl-product formed depend both particular substrate assayed PRMT modifies it. also find transient intracellular alkalinization mouse embryonic P19...
Overexpressed autofluorescent-tagged versions of the Fragile mental retardation protein (FMRP) such as EGFP-FMRP have been used in protein-protein interaction studies and composition, formation localization neuronal granules. However, question whether these molecules truly recapitulate properties endogenous has not addressed. Here we demonstrate that overexpressed forms three distinct granule types based on colocalization with various marker proteins. The majority EGFP-FMRP-containing...
Changes in cytoplasmic pH are known to regulate diverse cellular processes and influence neuronal activities. In neurons, the intracellular alkalization is shown occur after stimulating several channels receptors. For example, it has previously demonstrated P19 neurons that a sustained alkalinization can be mediated by Na(+)/H(+) antiporter. addition, benzodiazepine binding subtypes of γ-amino butyric acid type A (GABAA) receptor mediate transient when they stimulated. Because activities...
To investigate function of glycine receptors (GlyRs) at the hippocampal CA1 pyramidal cells and to characterize pharmacological properties these early postnatal stage.We used whole cell patch clamp recording study current response in acutely prepared slices from day 11-13 rats induced by applied artificial cerebrospinal fluid.Application elicited strychnine sensitive chloride currents. EC50 for GlyRs respond was 123. 23 μmol/L Hill coefficient 1.24. Picrotoxin could partly blocked...