A5002316528- Signaling Pathways in Disease
- Ubiquitin and proteasome pathways
- Parasitic Infections and Diagnostics
- Endoplasmic Reticulum Stress and Disease
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Aquaculture disease management and microbiota
- CRISPR and Genetic Engineering
- Marine Sponges and Natural Products
- Microbial Natural Products and Biosynthesis
- Protist diversity and phylogeny
- Heat shock proteins research
- Plant Disease Resistance and Genetics
- Echinoderm biology and ecology
- Protein Structure and Dynamics
- RNA Interference and Gene Delivery
- RNA and protein synthesis mechanisms
- RNA regulation and disease
- Toxoplasma gondii Research Studies
- interferon and immune responses
Boston University
2019-2023
Herein, transcription factor NF-κB is characterized from two model jellyfish, Aurelia aurita (Aa) and Clytia hemisphaerica (Ch). Both jellyfish proteins consist primarily of the N-terminal DNA-binding/dimerization domain, they lack C-terminal ankyrin repeat inhibitory domain found in vertebrate proteins. can bind to a consensus mammalian binding site, their AlphaFold3-predicted structures on DNA are similar that mouse p50. Neither Aa- nor Ch-NF-κB activated an NF-κB-site reporter gene human...
The use of alternative promoters for the cell type-specific expression a given mRNA/protein is common strategy. NEMO scaffold protein required canonical NF-κB signaling. Transcription gene primarily controlled by two promoters: one (promoter B) drives transcription in most types and second D) largely responsible liver cells. Herein, we have used CRISPR/Cas9-based approach to disrupt core sequence element promoter B, this genetic editing essentially eliminates mRNA 293T human kidney By...
Scaffold proteins help mediate interactions between protein partners, often to optimize intracellular signaling. Herein, we use comparative, biochemical, biophysical, molecular, and cellular approaches investigate how the scaffold NEMO contributes signaling in NF-κB pathway. Comparison of related optineurin from a variety evolutionarily distant organisms revealed that central region NEMO, called Intervening Domain (IVD), is conserved optineurin. Previous studies have shown this core IVD...
Scaffold proteins help mediate interactions between protein partners, often to optimize intracellular signaling. Herein, we use comparative, biochemical, biophysical, molecular, and cellular approaches investigate how the scaffold NEMO contributes signaling in NF-κB pathway. Comparison of related optineurin from a variety evolutionarily distant organisms revealed that central region NEMO, called Intervening Domain (IVD), is conserved optineurin. Previous studies have shown this core IVD...
We provide a functional characterization of transcription factor NF-κB in protists and information about the evolution diversification this biologically important protein. characterized two using phylogenetic, cellular, biochemical techniques. holozoan Capsaspora owczarzaki (Co) has an N-terminal DNA-binding domain C-terminal Ankyrin repeat (ANK) domain, its specificity is more similar to metazoan proteins than Rel proteins. Removal ANK allows Co-NF-κB enter nucleus, bind DNA, activate...
Abstract In this report, we investigate the evolution of transcription factor NF-κB by examining its structure, activity, and regulation in two protists using phylogenetic, cellular, biochemical techniques. Capsaspora owczarzaki ( Co ), find that full-length has an N-terminal DNA-binding domain a C-terminal Ankyrin (ANK) repeat inhibitory domain, activity is more similar to metazoan rather than Rel proteins. As with mammalian proteins, removal ANK repeats required for -NF-κB enter nucleus,...