A5000451784- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Collagen: Extraction and Characterization
- Cell Image Analysis Techniques
- Sports injuries and prevention
- Metabolism, Diabetes, and Cancer
- Advanced Fluorescence Microscopy Techniques
- Diet and metabolism studies
- Shoulder Injury and Treatment
- Pancreatic function and diabetes
- Tendon Structure and Treatment
- Single-cell and spatial transcriptomics
- Bone Tissue Engineering Materials
- MRI in cancer diagnosis
- Advanced Neuroimaging Techniques and Applications
- Barrier Structure and Function Studies
- Cancer Cells and Metastasis
- Muscle activation and electromyography studies
Universidad de Navarra
2014-2025
Navarre Institute of Health Research
2021-2025
Centro de Investigación Biomédica en Red de Cáncer
2017
Erasmus MC
2014
Heidelberg University
2014
University Hospital Heidelberg
2014
Abstract Motivation: Automatic tracking of cells in multidimensional time-lapse fluorescence microscopy is an important task many biomedical applications. A novel framework for objective evaluation cell algorithms has been established under the auspices IEEE International Symposium on Biomedical Imaging 2013 Cell Tracking Challenge. In this article, we present logistics, datasets, methods and results challenge lay down principles future uses benchmark. Results: The main contributions include...
Abstract The Cell Tracking Challenge is an ongoing benchmarking initiative that has become a reference in cell segmentation and tracking algorithm development. Here, we present significant number of improvements introduced the challenge since our 2017 report. These include creation new segmentation-only benchmark, enrichment dataset repository with datasets increase its diversity complexity, silver standard corpus based on most competitive results, which will be particular interest for...
Microfluidic devices are becoming mainstream tools to recapitulate in vitro the behavior of cells and tissues. In this study, we use microfluidic filled with hydrogels mixed collagen-Matrigel composition study migration lung cancer under different invasion microenvironments. We present design device, characterize morphologically mechanically quantitative image analysis measure H1299 adenocarcinoma experimental conditions. Our results show plasticity cell migration, which turns from...
The migration of cancer cells is highly regulated by the biomechanical properties their local microenvironment. Using 3D scaffolds simple composition, several aspects cell mechanosensing (signal transduction, EMC remodeling, traction forces) have been separately analyzed in context migration. However, a combined study these factors that more closely resemble complex microenvironment ECM still missing. Here, we present comprehensive, quantitative analysis role cell-ECM interactions within...
Astrocytes are considered an essential source of blood-borne glucose or its metabolites to neurons. Nonetheless, the necessity main astrocyte transporter, i.e., GLUT1, for brain metabolism has not been defined. Unexpectedly, we found that was paradoxically augmented in mice with astrocytic GLUT1 reduction (GLUT1 ΔGFAP mice). These also exhibited improved peripheral especially obesity, rendering them metabolically healthier. Mechanistically, observed GLUT1-deficient astrocytes increased...
Achilles tendon rupture is a frequent injury with an increasing incidence. After clinical surgical repair, aimed at suturing the stumps back into their original position, repaired often plastically deformed and mechanically less strong than pre-injured tissue, muscle fatty degeneration contributing to function loss. Despite outcomes, pre-clinical research has mainly focused on structural lack of knowledge regarding progression from its consequences degenerative/regenerative processes...
The geometry of 3D collagen networks is a key factor that influences the behavior live cells within extra-cellular matrices. This paper presents method for automatic quantification network with fiber resolution in confocal reflection microscopy images. proposed based on smoothing filter and binarization followed by reconstruction algorithm. validated gels various Matrigel concentrations. results reveal affects decreasing pore size while preserving length persistence length. influence...
The geometry of 3D collagen networks is a key factor that influences the behavior live cells within extracellular matrices. This paper presents hybrid, two-step method for fully automatic quantification network at fiber resolution in confocal reflection microscopy images. First, coarse binary mask entire obtained using steerable filtering and local Otsu thresholding. Second, individual fibers are reconstructed by tracing maximum ridges Euclidean distance map mask. proposed method, validated...
Abstract Astrocytes are considered an essential source of blood-borne glucose or its metabolites to neurons. Nonetheless, the necessity main astrocyte transporter, i.e. GLUT1, for brain metabolism has not been defined. Unexpectedly, we found that was paradoxically augmented in mice with astrocytic GLUT1 ablation (GLUT1 1′GFAP mice). These also exhibited improved peripheral especially obesity, rendering them metabolically healthier. Importantly, did present cognitive alterations....
Incorporating detailed muscle architecture aspects into computational models can enable researchers to gain deeper insights the complexity of function, movement, and performance. In this study, we employed histological, multiphoton image processing, finite element method techniques characterize mechanical dependency on architectural behavior supraspinatus infraspinatus mouse muscles. While tests revealed a stiffer passive in muscle, collagen content was found be two times higher...
The blood–brain barrier is a unique physiological structure acting as filter for every molecule reaching the brain through blood. For this reason, an effective pharmacologic treatment supplied to patient by systemic circulation should first be capable of crossing barrier. Standard cell cultures (or those based on microfluidic devices) and animal models have been used study human Unfortunately, these tools not yet reached state maturity because complexity process aggravated high heterogeneity...