A5101496162- Traditional Chinese Medicine Studies
- Neuroscience and Neuropharmacology Research
- Visual perception and processing mechanisms
- Ear Surgery and Otitis Media
- Receptor Mechanisms and Signaling
- Otitis Media and Relapsing Polychondritis
- Diabetes and associated disorders
- Ion channel regulation and function
- Lipid metabolism and disorders
- Monoclonal and Polyclonal Antibodies Research
- Facial Nerve Paralysis Treatment and Research
- Ophthalmology and Visual Impairment Studies
- Glycosylation and Glycoproteins Research
- Nanofabrication and Lithography Techniques
- Anesthesia and Neurotoxicity Research
- Corneal Surgery and Treatments
- Hydrogels: synthesis, properties, applications
- Traditional Chinese Medicine Analysis
- Spinal Fractures and Fixation Techniques
- Ecology and Conservation Studies
- Advanced Electron Microscopy Techniques and Applications
- Photoreceptor and optogenetics research
- Tracheal and airway disorders
- Vestibular and auditory disorders
- Single-cell and spatial transcriptomics
Guangzhou Regenerative Medicine and Health Guangdong Laboratory
2020-2025
Second Affiliated Hospital of Nanchang University
2024
Nanchang University
2024
Handan College
2023
Beijing Huajia University
2023
Sun Yat-sen University
2021
Huazhong University of Science and Technology
2020
Zhongshan Ophthalmic Center, Sun Yat-sen University
2019
Zhongshan Hospital
2019
University of California, San Diego
2003
Some structured RNAs, such as riboswitches and aptamers, can bind their cognate ligands have been used in biosensors gene expression control elements. However, current methods for detecting ligand binding to RNAs are either severely limited or inconvenient. In this study, we design a multi-base pair bridge integrate hammerhead ribozyme into detect events. The experimental results demonstrate that the length of has significant effect on cleavage ribozyme; optimal be achieved with three six...
Purpose: To explore whether subtle changes in visual quality can be detected using different measures of function against the quick contrast sensitivity test (quick CSF). Methods: Sixty participants, aged 17 to 34 years, were enrolled. Participants' vision was degraded by 0.25 D undercorrection (0.25 D), 60% neutral density filter brightness reduction (60% ND), and 0.8 Bangerter foil optical diffusion (0.8BAN). Visual tests including acuity (CSV-1000E CSF) measured with participant's...
G protein-coupled receptors (GPCRs) are a large family of cell membrane proteins that important targets for drug discovery. Nanobodies, also known as VHH (variable domains heavy chain-only antibodies, HcAbs) small antibody fragments derived from camelids have gained significant attention potential therapeutics targeting GPCRs due to their advantages over conventional antibodies. However, there challenges in developing nanobodies GPCRs, among which epitope accessibility is the most because...
Arnebiae Radix (dried root of Arnebia euchroma (Royle) Johnst.) is a traditional Chinese medicine (TCM) used to treat macular eruptions, measles, sore throat, carbuncles, burns, skin ulcers, and inflammations. The extract can exert anti-breast cancer effects through various mechanisms action. This study aimed rapidly screen potential estrogen receptor (estrogen α β) ligands from the extract. In this study, an analytical method based on affinity ultrafiltration coupled with UHPLC-Q-Exactive...
Polo-like kinase 4 (PLK4) involves in tumor progression via regulating centriole duplication. This study aimed to investigate correlations of PLK4 with characteristics and survival cutaneous melanoma patients undergoing surgical resection. Tumor specimens 43 were retrieved for determination by immunohistochemistry (IHC). The IHC score was a multiplication staining intensity percentage staining-positive cells. found the median mean 0.0 (interquartile range: 0.0-6.0) 3.5 ± 3.2 (mean SD),...
Cell surface trafficking of many G protein-coupled receptors is tightly regulated. Among them, the mandatory heterodimer GABAB receptor for main inhibitory neurotransmitter, γ-aminobutyric acid (GABA), a model. In mammals, its cell highly controlled by an endoplasmic reticulum retention signal in C-terminal intracellular region GB1 subunit that masked through coiled-coil interaction with GB2 subunit. Here, we investigate molecular basis export homolog Drosophila melanogaster regulates...
Contrast sensitivity (CS) is important when assessing functional vision. However, current techniques for CS are not suitable young children or non-verbal individuals because they require reliable, subjective perceptual reports. This study explored the feasibility of applying eye tracking technology to quantify as a first step toward developing testing paradigm that will rely on observers’ behavioral language abilities. Using within-subject design, 27 healthy adults completed measures three...
Human pepsinogens (mainly pepsinogen I and II) are the major inactive precursor forms of digestive enzyme pepsin which play a crucial role in protein digestion. The levels ratios human have demonstrated potential as diagnostic biomarkers for gastrointestinal diseases, particularly gastric cancer. Nanobodies promising tools treatment diagnosis owing to their unique recognition properties. In this study, recombinant proteins were expressed purified immunized antigens. We constructed VHH phage...
Abstract Metabotropic GABA B G protein-coupled receptor functions as a mandatory heterodimer of GB1 and GB2 subunits mediates inhibitory neurotransmission in the central nervous system. Each subunit is composed extracellular Venus flytrap (VFT) domain transmembrane (TM) domain. Here we present cryo-EM structures human full-length heterodimeric antagonist-bound inactive state active complexed with agonist positive allosteric modulator presence i1 protein at resolution range 2.8-3.0 Å. Cryo-EM...
As an increasingly prevalent mental disorder around the globe, depression influences more than 300 million people and attract attention of researchers to find out a clear reliable mechanism. One largest influencers in human body, gastrointestinal microbiota has raised serve as clue explain onset depression. The evidence from previous studies is summarized support that difference gut does correlate significantly with development Supported by result variety including animal study prospective...
Journal Article Informatics Resources for Electron Tomography Get access Maryann E Martone, Martone National Center Microscopy and Imaging Research, Research in Biological Structure Dept. of Neurosciences, University California, San Diego, La Jolla, CA 92093-0608San Diego Supercomputer Center, 92093-0505, USA Search other works by this author on: Oxford Academic Google Scholar Shenglan Zhang, Zhang Steven Peltier, Peltier 92093-0608 Abel W Lin, Lin David Lee, Lee Tomas Molina, Molina...