A5070859698- Pancreatic function and diabetes
- Lung Cancer Treatments and Mutations
- Diabetes and associated disorders
- Xenotransplantation and immune response
- HER2/EGFR in Cancer Research
- Colorectal Cancer Treatments and Studies
- Diabetes Management and Research
- Cancer Genomics and Diagnostics
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Chronic Myeloid Leukemia Treatments
- Tissue Engineering and Regenerative Medicine
- Pancreatic and Hepatic Oncology Research
- Pancreatitis Pathology and Treatment
- Nephrotoxicity and Medicinal Plants
- Medical Imaging Techniques and Applications
- Bone Tissue Engineering Materials
- Drug-Induced Hepatotoxicity and Protection
- Cannabis and Cannabinoid Research
- Head and Neck Cancer Studies
- Renal Transplantation Outcomes and Treatments
- Renal Diseases and Glomerulopathies
- Organ Transplantation Techniques and Outcomes
- Radiopharmaceutical Chemistry and Applications
- Reconstructive Surgery and Microvascular Techniques
- Renal and Vascular Pathologies
UCLouvain
2004-2023
Cliniques Universitaires Saint-Luc
1998-2019
KU Leuven
1987-2008
Decreases in both beta-cell function and number can contribute to insulin deficiency type 2 diabetes. Here, we quantified the mass pancreas obtained at autopsy of 57 diabetic (T2D) 52 non-diabetic subjects European origin. Sections from body tail were immunostained for insulin. The was calculated volume density beta-cells (measured by point-counting methods) weight pancreas. pancreatic concentration measured some subjects. increased only slightly with index (BMI). After matching BMI, 41%...
This study assessed the capacity of alginate-encapsulated islets to reverse diabetes in a pig-to-primate model.Adult pig were encapsulated microcapsules implanted under kidney capsule (n=4) or subcutaneous macrodevice (n=5) diabetic primates. Fasting blood glucose (FBG), insulin, porcine C-peptide, glycosylated hemoglobin (HbA1C), and cellular humoral responses followed.Nonencapsulated rejected within 7 days. A transient decrease FBG was observed only during 2 weeks after microencapsulated...
Pig islets xenotransplantation remains associated with a strong humoral and cellular xenogeneic immune responses. The aim of this study was to assess the long-term biocompatibility alginate encapsulated pig after transplantation in primates.Adult under optimal conditions (n=7) or not (n=5) were transplanted kidney capsule nondiabetic Cynomolgus maccacus. Additional primates received empty capsules (n=1) nonencapsulated (n=2) as controls. Capsule integrity, overgrowth, islet survival, porcine...
Background. To induce irreversible diabetes in large animals, the efficiency of streptozotocin (STZ) was evaluated pigs, primates and compared to gold standard model rats. Methods. Low (50 mg/kg) high (150 doses STZ were tested. Hepatic/renal function, glucose metabolism (intravenous tolerance tests, fasting blood glucose) histomorphometry prior to, 1, 4 weeks after treatment. Results. In rats primates, expressing a level GLUT2 expression on β cells, dose 50 mg/kg induced (due 97%...
Islet encapsulation requires several properties including (1) biocompatibility, (2) immunoprotection, and (3) oxygen diffusion for islet survival diabetes correction. New chemical alginates were tested in vivo compared with traditional high-mannuronate -guluronate alginates. coupled peptide sequence (sterile lyophilized high mannuronate [SLM]-RGD3% sterile guluronate [SLG]-RGD3%), to improve encapsulated cell adherence the matrix, a very low viscosity (VLDM7% VLDG7%), reduce implant size by...
Dufrane D, D'hoore W, Goebbels R‐M, Saliez A, Guiot Y, Gianello P. Parameters favouring successful adult pig islet isolations for xenotransplantation in pig‐to‐primate models. Xenotransplantation 2006; 13: 204–214. © Blackwell Munksgaard, 2006 Abstract: Background: In the near future, porcine islets of Langerhans appear as an unlimited source insulin‐producing cells which could play a major role treating diabetes mellitus. There is, however, obvious lack pre‐clinical results and data model....
To study the impact of porcine islet size on structural properties and cellular engraftment.The endocrine structure collagen/vascular localization in pig islets were studied before after enzymatic isolation pancreas from 6 young adult Landrace pigs. Isolated both types transplanted under kidney capsula diabetic nude rats to assess engraftment.In comparison with pancreata, a significantly greater number small beta cells (<100 microm) observed (82% vs. 32%, respectively, P < 0.005) pancreata....
Isolation of large numbers intact and functional human islets remains difficult expensive. We describe a novel method using polymethylpenten chamber (PMPC) compare its efficacy to the classic stainless steel (SSC).Five pancreases obtained from cadaveric donors were processed with SSC method, purified Cobe cell separator. The next 15 (similar donor characteristics) distended Liberase HI, minced, digested in PMPC whose thermic properties did not require continuous heating maintain temperature...
Optimal head and neck squamous cell carcinoma (HNSCC) patient selection for anti-EGFR-based therapy remains an unmet need since only a minority of patients derive long-term benefit from cetuximab treatment. We assessed the ability state-of-the-art noninvasive in vivo metabolic imaging to probe shift cetuximab-sensitive -resistant HNSCC patient-derived tumor xenografts (PDTXs).Three models selected based on their known sensitivity (cetuximab-sensitive or acquired-resistant HNC007 PDTXs,...
Abstract Purpose: The EORTC-90111–24111 phase II window study evaluated afatinib versus no preoperative treatment in patients with primary squamous cell carcinoma of the head and neck (HNSCC). We investigated afatinib-induced tumor microenvironment modifications by comparing pre- posttreatment biopsies. Patients Methods: Thirty treatment-naïve HNSCC were randomized. Twenty-five received for 14 days before surgery (40 mg 1×/day) 5 attributed to control arm. Biopsies taken at work-up during...
Abstract: Background: Metabolic compatibility between donor and recipient species is an important matter for pig islet xenotransplantation. Glucagon a key hormone the function of islets as well control hypoglycemia in recipients islets. Because discrepancy exists composition glucagon cells human/primate islets, present study was designed to determine role native treatment diabetes by transplantation “pig‐to‐primate” model. Methods: Streptozotocin‐treated (50 mg/kg) monkeys (n = 12, follow‐up...
We developed a composite scoring system to accurately assess pig islet function in pre-clinical primate studies.Two methods that have been clinically validated human allotransplantation were tested six non-diabetic and nine streptozotocin (STZ)-induced diabetic primates: (i) SUITO index=[1500 × fasting C-peptide (ng/ml)]/[fasting blood glucose (FBG, mg/dl) - 63] (ii) CP/G ratio =[fasting (ng/ml) 100]/FBG (mg/dl). Both scores analysed as of the β-cell mass native pancreas. Next, proposed β5...
We investigated changes on 2'-deoxy-2'-[18F]fluoro-D-glucose positron emission tomography (18FDG-PET), diffusion-weighted magnetic resonance imaging (DW-MRI), and choline spectroscopy as early markers of cetuximab activity in squamous cell carcinoma the head neck (SCCHN). SCCHN patient-derived tumor xenografts models were selected based their sensitivity. Three resistant to two sensitive (one was highly other one moderately sensitive). Cetuximab infused day 0 7. Maximal standardized uptake...