A5110503836- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Viral Infections and Immunology Research
- Infectious Diseases and Tuberculosis
- Neural Networks and Applications
- RNA and protein synthesis mechanisms
- Essential Oils and Antimicrobial Activity
- Animal Disease Management and Epidemiology
- Viral gastroenteritis research and epidemiology
- Text and Document Classification Technologies
- Topic Modeling
- Bacteriophages and microbial interactions
- Cytomegalovirus and herpesvirus research
- Machine Learning in Healthcare
- Bacterial Identification and Susceptibility Testing
- Privacy-Preserving Technologies in Data
- Whipple's Disease and Interleukins
- Machine Learning and ELM
- Fuzzy Logic and Control Systems
- Cell Image Analysis Techniques
- Artificial Intelligence in Healthcare
- Streptococcal Infections and Treatments
- Biomedical Text Mining and Ontologies
- Mobile Agent-Based Network Management
- Advanced Sensor and Control Systems
Yale University
2024
Kaohsiung Veterans General Hospital
2010-2023
Institute of Biomedical Sciences, Academia Sinica
2017
Fooyin University
2007-2014
National Yang Ming Chiao Tung University
2008-2014
National Taipei University
2012
National Yang Ming University Hospital
2008
National Chung Hsing University
1994
Diseases caused by infectious and inflammatory microorganisms are among the most common severe nosocomial diseases worldwide. Therefore, developing effective agents for treating these illnesses is critical. In this study, essential oils from two tea tree species, kanuka (Kunzea ericoides) manuka (Leptospermum scoparium), were evaluated use in inflammation microorganism infection.Isolates of clinically bacteria fungi obtained American Type Culture Collection Kaohsiung Veterans General...
The Mycobacterium tuberculosis (MTB) Beijing strain is highly virulent, drug resistant, and endemic over Asia. To explore the genetic diversity of this family in several different regions eastern Asia, 338 strains collected Taiwan (Republic China) were analyzed by mycobacterial interspersed repetitive unit-variable number tandem repeat (MIRU-VNTR) typing compared with published MIRU-VNTR profiles Hunter-Gaston index (HGDI) from Japan South Korea. results revealed that VNTR2163b (HGDI>0.6)...
This current work was to investigate the biological effects of acidic cosmetic water (ACW) on various assays. ACW isolated from seawater and demonstrated several bio-functions at concentration ranges. showed a satisfactory effect against Staphylococcus aureus, which reduced 90% bacterial growth after 5-second exposure. We used cultured human peripheral blood mononuclear cells (PBMCs) test properties in inflammatory cytokine release, it did not induce release un-stimulated, normal PBMCs....
We present the first comprehensive analysis of Mycobacterium tuberculosis isolates circulating in Kaohsiung region southern Taiwan. The major spoligotypes found 224 studied were Beijing lineages (n = 97; 43.3%), EAI 72; 32.1%) and Haarlem 18; 8.0%). By 24 MIRU-VNTR typing, 174 patterns identified, including clusters 74 150 unique patterns. combination spoligotyping 12-MIRU-VNTR revealed that 129 (57.6%) clustered 18 genotypes. Moreover, 63.6% (7/11) infected persons younger than 30 years had...
The infectious activity of coxsackievirus B1 (CV-B1) in Taiwan was high from 2008 to 2010, following an alarming increase severe neonate disease the United States (US). To examine relationship between CV-B1 strains isolated and those other parts world, we performed a phylodynamic study using VP1 partial 3Dpol (414 nt) sequences 22 (1989–2010) compared them worldwide. Phylogenetic trees were constructed by neighbor-joining, maximum likelihood, Bayesian Monte Carlo Markov Chain methods. Four...
Background We present the first comprehensive analysis of Mycobacterium tuberculosis (MTB) isolates circulating in southern Taiwan. In this 9-year population-based study, TB situation Kaohsiung region was characterized by genotypic 421 MTB isolates. Methods All were analyzed spoligotyping and MIRU-VNTR typing. Drug-resistance patterns also analyzed. Results The percentage EAI (East African-Indian) strains increased across sampling years (2000–2008) Taiwan, whereas proportion Beijing lineages...
This study addresses the challenge of accurately identifying filamentous fungi in medical laboratories using transfer learning with convolutional neural networks (CNNs). The uses microscopic images from touch-tape slides lactophenol cotton blue staining, most common method clinical settings, to classify fungal genera and identify Aspergillus species. training test data sets included 4,108 representative morphology for each genus, a soft attention mechanism was incorporated enhance...
Although polymerase chain reaction (PCR) is a highly sensitive procedure for the diagnosis of enteroviruses, it has never been systemically applied to treatment enteroviral encephalitis using intravenous immunoglobulin (IVIg). We conducted 2-year randomized, controlled comparison reverse transcription (RT)-PCR cerebrospinal fluid (CSF) with traditional viral isolation guide IVIg treatment. Seventy-five patients were enrolled and classified into three groups: one group clinical manifestations...
Studies regarding coxsackievirus (CV) tend to focus on epidemic outbreaks, an imbalanced topology is considered be indication of acute infection with partial cross-immunity. In enteroviruses, a clear understanding the characteristics tree topology, transmission, and its demographic dynamics in viral succession circulation are essential for identifying prevalence trends endemic pathogens such as B2 (CV-B2). This study applied novel Bayesian evolutionary approach elucidate phylodynamic CV-B2....
Supercritical fluid carbon dioxide extraction technology was developed to gain the active components from a Taiwan native plant, Zingiber officinale (ginger). We studied biological effects of ginger extracts via multiple assays and demonstrated biofunctions in each platform. Investigations indicated antioxidative properties dose‐dependant manners on radical scavenging activities, reducing powers metal chelating powers. found that processed moderate values, middle levels, slight ferric The...
To understand human parechovirus (HPeV) infections in Taiwanese children, we analyzed data for 112 children (age≤10 years) with HPeV infection diagnosed between July 2007 and June 2016 a medical center Kaohsiung, southern Taiwan. The patients were infected HPeV1 (n=94), HPeV3 (n=3), HPeV4 HPeV6 (n=1) non-typeable (n=11). We compared the clinical implications younger than 3 months (n=56) older (n=31), excluding 25 concomitant infections. Fever was noted almost half of but more frequent (83.9%...
Coxsackievirus (CV)-B5 is a common human enterovirus reported worldwide; swine vesicular disease virus (SVDV) porcine variant of CV-B5. To clarify the transmission dynamics and molecular basis host switching between CV-B5 SVDV, we analysed compared VP1 partial 3Dpol gene regions these two viruses. Spatiotemporal viral were estimated using Bayesian statistical inference framework. The detected selection events used to analyse key molecules associated with switching. Analyses sequences...
For tiling of the SARS-CoV-2 genome, ARTIC Network provided a V4 protocol using 99 pairs primers for amplicon production and is currently widely used amplicon-based approach. However, this technique has regions low sequence coverage labour-, time-, cost-intensive. Moreover, it requires 14 in two separate PCRs to obtain spike gene sequences. To overcome these disadvantages, we proposed single PCR efficiently detect mutations. We bioinformatic that can process FASTQ reads into consensus...
A point-of-use 0.2-microm filter was evaluated for elimination of nontuberculosis mycobacteria in laboratory water to reduce false-positive acid-fast bacillus staining results. Use the can significantly rate 1.2% compared samples treated with tap (10.7%) and deionized (8.7%).
<b><i>Objectives:</i></b> We aimed to utilize a simple molecular assay simultaneously detect both group B <i>Streptococcus</i> (GBS) and virulent ST-17 rectovaginal colonization. also attempted estimate the prevalence of maternal GBS carriers evaluate their seasonal association. <b><i>Subjects Methods:</i></b> used an optimized multiplex PCR method employing scp-B primers analyze DNA extracted from swabs 3,064 cases collected over 3...
Tuberculosis remains a major infectious disease in Taiwan. Here we present the draft genome sequence of Mycobacterium tuberculosis C2 strain, belonging to Latin American-Mediterranean lineage. The comprises 4,453,307 bp with G+C content 65.6%, revealing 4,390 coding genes and 45 tRNA genes.
The recurrence rate of Mycobacterium tuberculosis in Taiwan is 3%. Here, we present the draft genome sequences M. strains A2 and A4 from a relapse patient. comprise 4,443,031 bp 4,487,096 bp, revealing 4,220 4,143 coding for A4, respectively, as well 49 tRNA genes both isolates.
The authors propose an online Chinese signature verification method based on one-bit quantized pressure patterns which are the time domain information. performance analysis was a set of ninety signatures from nine persons over two-month period and ten traced forgeries obtained forgers. Three experiments were analyzed in terms type I II error probabilities. results suggest that timing information presented can be used for first stage screening incoming by using ART1 neural network with...
Aichi virus (AiV) belongs to the genus Kobuvirus of family Picornaviridae; it is a single-stranded positive-sense RNA without an envelope. AiV causes acute gastroenteritis, abdominal pain, nausea, vomiting, and fever. Low incidence high seroprevalence infections have been reported in several regions world; however, little was known on prevalence Taiwan. This study described first two cases infection analyzed A total 700 sera were collected from single hospital southern The neutralization...