A5079206152- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- RNA modifications and cancer
- Histone Deacetylase Inhibitors Research
- Cancer, Hypoxia, and Metabolism
- Moyamoya disease diagnosis and treatment
- Metastasis and carcinoma case studies
- Sirtuins and Resveratrol in Medicine
- Adenosine and Purinergic Signaling
- Cancer-related Molecular Pathways
- Pancreatic and Hepatic Oncology Research
- Cancer Mechanisms and Therapy
- Mechanisms of cancer metastasis
- MicroRNA in disease regulation
- Ferrocene Chemistry and Applications
- Macrophage Migration Inhibitory Factor
- Aortic Disease and Treatment Approaches
- Ubiquitin and proteasome pathways
- Calcium signaling and nucleotide metabolism
- Mitochondrial Function and Pathology
- Autophagy in Disease and Therapy
- Cancer Research and Treatments
- Adipose Tissue and Metabolism
- Cancer-related molecular mechanisms research
Jinan Central Hospital
2024
Shandong First Medical University
2024
Tianjin Medical University
2018-2021
Lovelace Respiratory Research Institute
2014
Histone post–translational modifications regulate chromatin structure and function largely through interactions with effector proteins that often contain multiple histone-binding domains. PHF1 [plant homeodomain (PHD) finger protein 1], which contains two kinds of histone reader modules, a Tudor domain PHD fingers, is an essential factor for epigenetic regulation genome maintenance. While significant progress has been made in characterizing the domain, roles fingers are poorly defined. Here,...
Lysine-specific demethylase 1 (LSD1) was the first histone identified as catalysing removal of mono- and di-methylation marks on H3-K4. Despite potential broad action LSD1 in transcription regulation, recent studies indicate that may coordinate with multiple epigenetic regulatory complexes including CoREST/HDAC complex, NuRD SIRT1, PRC2, implying complicated mechanistic actions this seemingly simple enzyme. Here, we report is also an integral component SIN3A/HDAC complex. Transcriptional...
The histone methyl transferase enhancer of zeste homolog 2 (EZH2) is a master transcriptional regulator involved in H3 lysine 27 trimethylation. We aimed to elucidate the precise post-translational regulations EZH2 and their role cancer pathogenesis. Here, we show that SET MYND domain containing (SMYD2) directly methylates at 307 (K307) enhances its stability, which can be relieved by H3K4 demethylase lysine-specific 1 (LSD1). SMYD2 critical for function repressing cohort genes governing...
Abstract The biological function of PRMT5 remains poorly understood in cervical cancer metastasis. Here, we report that physically associates with the transcription factor Snail and NuRD(MTA1) complex to form a transcriptional-repressive catalyzes symmetrical histone dimethylation deacetylation. This study shows Snail/PRMT5/NuRD(MTA1) targets genes, such as TET1 E-cadherin , which are critical for epithelial-mesenchymal transition (EMT). also affects conversion 5mC 5hmC. demonstrates...
Pancreatic cancer is a common malignant tumor with poor prognosis. Recently, stem cells (CSCs) were identified in several solid tumors, including pancreatic cancer. Although accumulating evidence indicates that sirtuin 1 (SIRT1) exerts biological functions various cancers, how it contributes to tumorigenesis and metastasis of cancer, as well its role CSCs, still poorly defined. Here we show SIRT1 interacts the Cullin 4B (CUL4B)-Ring E3 ligase (CRL4B) complex, which responsible for H2AK119...
Abstract GATA3 has emerged as a prominent transcription factor required for maintaining mammary-gland homeostasis. loss is associated with aggressive breast cancer development, but the mechanism by which affected of function remains unclear. Here, we report that expression positively correlated UTX, histone H3K27 demethylase contained in MLL4 methyltransferase complex, and recruits chromatin-remodeling complex interacts directly ASH2L, RBBP5. Using RNA sequencing chromatin...
Cullin4B (CUL4B) is a scaffold protein of the CUL4B-Ring E3 ligase (CRL4B) complex. However, role CUL4B in development breast cancer remains poorly understood. Here it shown that CRL4B interacts with multiple histone deacetylase (HDAC)-containing corepressor complexes, including MTA1/NuRD, SIN3A, CoREST, and NcoR/SMRT complexes. It demonstrated CRL4B/NuRD(MTA1) complexes cooccupy E-cadherin AXIN2 promoters, could be recruited by transcription factors Snail ZEB2 to promote cell invasion...
The homeodomain transcription factor SIX3 was recently reported to be a negative regulator of the Wnt pathway and has an emerging role in cancer. However, how contributes tumorigenesis metastasis is poorly understood.We employed affinity purification mass spectrometry (MS) identify proteins physically associated with SIX3. Genome-wide analysis SIX3/LSD1/NuRD(MTA3) complex using chromatin immunoprecipitation-on-chip approach identified cohort target genes including WNT1 FOXC2, which are...
Aortic aneurysm/dissection (AAD) is a serious cardiovascular condition characterized by rapid onset and high mortality rates. Currently, no effective drug treatment options are known for AAD. AAD pathogenesis associated with the phenotypic transformation abnormal proliferation of vascular smooth muscle cells (VSMCs). However, endogenous factors that contribute to progression remain unclear. We aimed investigate role histone deacetylase 9 (HDAC9) in pathogenesis. HDAC9 expression was...
BACKGROUND: The occurrence of thoracic aortic dissection (TAD) is closely related to the transformation vascular smooth muscle cells (VSMCs) from a contractile synthetic phenotype. role SGK1 (serum- and glucocorticoid-regulated kinase 1) in VSMC phenotypic TAD unclear. METHODS: Four-week-old male Sgk1 F/F ( floxed) ;Tagln Cre (smooth cell–specific knockout) mice were administered β-aminopropionitrile monofumarate for 4 weeks model TAD. inhibitor GSK650394 was daily via intraperitoneal...
Objective: To investigate the role of CUL4B-RING E3 ubiquitin ligase (CRL4B) complex in pancreatic tumorigenesis and molecular mechanism. Methods: Pancreatic cells were divided into control group (transfected with negative lentivirus), shCUL4B CUL4B shDDB1 [transfected DNA damage binding protein 1 (DDB1) lentivirus], shCUL4B+ siSFRP1 lentivirus SFRP1-siRNA). RNA-seq was performed cancer cell lines DDB1 knocked down respectively, to identify target genes regulated by CRL4B complex. Real-time...