A5101457875- Advanced biosensing and bioanalysis techniques
- HIV Research and Treatment
- RNA and protein synthesis mechanisms
- HIV/AIDS drug development and treatment
- RNA modifications and cancer
- RNA Interference and Gene Delivery
- Biosensors and Analytical Detection
- Immunotherapy and Immune Responses
- vaccines and immunoinformatics approaches
- Genetic Mapping and Diversity in Plants and Animals
- Extracellular vesicles in disease
- Cancer, Hypoxia, and Metabolism
- Computational Drug Discovery Methods
- Advanced Biosensing Techniques and Applications
- Epigenetics and DNA Methylation
- Peroxisome Proliferator-Activated Receptors
- Single-cell and spatial transcriptomics
- Cancer Research and Treatments
- Glioma Diagnosis and Treatment
- Nanoparticle-Based Drug Delivery
- RNA Research and Splicing
- Herpesvirus Infections and Treatments
- Click Chemistry and Applications
- Nanopore and Nanochannel Transport Studies
- Immune Cell Function and Interaction
Shanghai Center For Bioinformation Technology
2024-2025
Beijing Institute of Genomics
2022-2025
Chinese Academy of Sciences
2003-2025
University of Chinese Academy of Sciences
2022-2025
Cangzhou Central Hospital
2024
Tianjin Medical University Cancer Institute and Hospital
2024
Changchun Institute of Applied Chemistry
2022-2024
Zhejiang University
2024
Nanjing Medical University
2024
Harbin Medical University
2021-2023
The National Genomics Data Center (NGDC), part of the China for Bioinformation (CNCB), provides a family database resources to support global academic and industrial communities. With explosive accumulation multi-omics data generated at an unprecedented rate, CNCB-NGDC constantly expands updates core by big archive, integrative analysis value-added curation. In past year, efforts have been devoted integrating multiple omics data, synthesizing growing knowledge, developing new upgrading set...
ABSTRACT The development of a human immunodeficiency virus type 1 (HIV-1) vaccine that elicits potent cellular and humoral immune responses recognizing divergent strains HIV-1 will be critical for combating the global AIDS epidemic. present studies were initiated to examine magnitude breadth envelope (Env)-specific T-lymphocyte antibody generated by vaccines containing either single or multiple genetically distant Env immunogens. Rhesus monkeys immunized with DNA prime-recombinant adenovirus...
Because a strategy to elicit broadly neutralizing anti-human immunodeficiency virus type 1 (HIV-1) antibodies has not yet been found, the role of an Env immunogen in HIV-1 vaccine candidates remains undefined. We sought determine whether genetically disparate from challenge can contribute protective immunity. vaccinated Indian-origin rhesus monkeys with Gag-Pol-Nef immunogens, alone or combination immunogens that were either matched mismatched virus. These animals then challenged pathogenic...
Metal–organic frameworks (MOFs) receive more and interest in the field of analytical chemistry for their diverse structures multifunctionality. In this study, we have designed fabricated nanoscale MOF-based nanoprobes multicolor detection DNA targets with improved sensitivity. To do so, nanoprobes, constructed by using porous MOFs as a scaffold to load signal dyes hairpin structure capping shell, been prepared. Once target has introduced, competitive displacement reaction triggers release...
SF3B1 is the most frequently mutated spliceosomal gene in cancer. Several hotspot mutations are known to disrupt interaction of with another splicing factor, SUGP1, resulting RNA missplicing that characterizes mutant cancers. Properties especially presence a G-patch motif, structure function by activating DEAH-box helicases, suggest requirement such an enzyme SUGP1 splicing. However, identity this putative helicase has remained important unanswered question. Here, using variety...
Capture and analysis of circulating tumor cells (CTCs) from complex matrixes is pivotal for the prediction cancer metastasis personalized treatment cancer. Herein, we propose a strategy CTC capture by design fabrication polyvalent aptamer network on an electrode surface, which can be further used sensitive CTCs. In our design, network, constructed via rolling circle amplification reaction, significantly enhance cell-binding abilities. Meanwhile, tetrahedral DNA structures previously...
ABSTRACT Immunity to human immunodeficiency virus virion-like structures or a polyprotein has been examined after DNA immunization with Rev-independent expression vectors. A Gag-Pol fusion protein stimulated cytotoxic T lymphocyte and antibody responses Gag Pol, while pseudoparticle did not elicit substantial Pol responses. This may be useful for AIDS vaccines.
Immunization with recombinant serotype 5 adenoviral (rAd5) vectors or a combination of DNA plasmid priming and rAd5 boosting is known to elicit potent immune responses. However, little data exist regarding these immunization strategies the development anti-human immunodeficiency virus type 1 (HIV-1) neutralizing antibodies. We used plasmids encoding HIV-1 89.6P chimeric HxB2/BaL envelope glycoprotein immunize macaque monkeys. A single elicited anti-Env antibody responses, but there was...
Pituitary neuroendocrine tumors (PitNETs) are one of the most common intracranial with diverse clinical manifestations. Current pathological classification systems rely primarily on histological hormone staining and transcription factors (TFs) expression. While effective in identifying three major lineages, molecular characteristics based hormones TFs lack sufficient resolution to fully capture complex tumor heterogeneity. Transcriptional diversity by alternative splicing (AS) offered...
The ability to elicit an immune response a spectrum of human immunodeficiency virus type 1 (HIV-1) gene products from divergent strains is desirable feature AIDS vaccine. In this study, we examined combinations plasmids expressing multiple HIV-1 genes different clades for their humoral and cellular responses in mice. Immunization with modified Env, gp145DeltaCFI, combination Gag-Pol-Nef fusion protein plasmid elicited similar CD4(+) CD8(+) immunization either vector alone. Further, when mice...
Human immunodeficiency virus (HIV) particles produced in COS-7 cells transfected with HIV type 1 (HIV-1) proviral DNA contain 8 molecules of tRNA(3Lys) per 2 genomic RNA and 12 tRNA1,2Lys RNA. When are a plasmid containing both HIV-1 human tRNA3Lys gene, there is large increase the amount cytoplasmic microgram total cellular RNA, content increases from to 17 However, number tRNALys remains constant at 20; i.e., viral decreases 3 All detectable aminoacylated cytoplasm infected deacylated...
A well-designed DNA tetrahedron based biosensor (DTB) has been employed for imaging and detection of argonaute2 (Ago2), a key RNA interference (RNAi) protein. This DTB mainly contains two segments: as frame photoinduced electron-transfer (PET) pair fluorescence transducer. The tetrahedral nanostructure is assembled with four nucleic acid strands. On one edge, strand forms hairpin structure (RNA sequence) which implanted in the complementary double-strand DNAs, PET pairs, DNA/silver...
Alternative splicing (AS) is a fundamental process that governs almost all aspects of cellular functions, and dysregulation in this has been implicated tumor initiation, progression treatment resistance. With accumulating studies carcinogenic mis-splicing cancers, there an urgent demand to integrate cancer-associated changes better understand their internal cross-talks functional consequences from global view. However, resource key AS events human cancers still lacking. To fill the gap, we...
Abstract A prophylactic vaccine for HIV-1 will probably require the induction and maintenance of both humoral cellular immunity. One current strategy to achieve such long term immune responses is a prime-boost vaccination approach using DNA priming inoculation, followed by recombinant viral boost. In this report we use novel in which injections consist Gag protein mixed with cytosine phosphate guanosine oligodeoxynucleotide (CpG ODN), adenoviral boost expressing Gag. Analysis indicates that...
Multispecific antibodies (MsAbs) maintain the specificity of versatile while simultaneously addressing different epitopes for a cumulative, collaborative effect. They could be an alternative treatment to chimeric antigen receptor-T cell therapy by helping redirect T cells tumors in vivo. However, one major limitation their development is relatively complex production process, which involves performance massive screen with low yield, inconsistent quality, and nonnegligible impurities. Here,...
In human immunodeficiency virus type 1, tRNALys3 is placed upon the primer binding site, where it serves as for reverse transcription. We show herein that genomic placement occurs independently of precursor protein processing and exists in two major forms: unextended or a form extended by first DNA bases incorporated, C T. The two-base absent protease-negative mutant, indicating requirement mature viral proteins to initiate transcription within virus.
ABSTRACT During human immunodeficiency virus type 1 (HIV-1) assembly, the primer tRNA for reverse transcriptase-catalyzed synthesis of minus-strand strong-stop cDNA, 3 Lys , is selectively packaged into and annealed onto binding site on RNA genome. Annealing in HIV-1 independent polyprotein processing facilitated vitro by p7 nucleocapsid (NCp7). We have previously shown that mutations clusters basic amino acids flanking first Cys-His box NC sequence inhibit annealing vivo 70 to 80%. In this...
During HIV-1 viral assembly, both Pr160gag-pol and primer tRNALys3 are packaged into the virus. tRNALys packaging (both tRNALys1,2) is dependent upon presence of RT sequences within Pr160gag-pol. In this work, we have monitored effect mutations incorporation produced from COS-7 cells transfected with mutant proviral DNAs. Mutations include carboxy deletions small amino acid insertions replacements various functional domains reverse transcriptase (RT). was by 2D PAGE RNA, hybridization...
COS-7 cells were transfected with DNAs containing mutations in the NCp7 sequences of human immunodeficiency virus. Selective incorporation into virus tRNA(Lys) was measured by two-dimensional polyacrylamide gel electrophoresis, and Pr160(gag-pol) detected Western blots viral protein. Mutations tested included cysteine histidine either Cys-His boxes, as well N- C-terminal flanking regions linker region between two boxes. Of 10 tested, only 2 inhibited incorporation: a P31L mutation deletion...
tRNA(3Lys) is a primer for reverse transcription in human immunodeficiency virus type 1 (HIV-1), and the anticodon of has been implicated playing role both its placement onto HIV-1 genome interaction with transcriptase (RT). In this work, gene was changed from UUU to CUA (tRNA(3Lys)Su+) or, addition, G-73 altered A (tRNA(3Lys)Su+G73A). COS-7 cells were transfected either wild-type or mutant genes, produced purified by using immobilized tRNA-specific hybridization probes. Each tested ability...