A5006897953- Extracellular vesicles in disease
- Milk Quality and Mastitis in Dairy Cows
- MicroRNA in disease regulation
- Tissue Engineering and Regenerative Medicine
- COVID-19 Clinical Research Studies
- Immune Response and Inflammation
- Viral Infections and Vectors
- Cell Adhesion Molecules Research
- interferon and immune responses
- RNA Interference and Gene Delivery
- Air Quality and Health Impacts
- SARS-CoV-2 and COVID-19 Research
- Immunotherapy and Immune Responses
- Infant Nutrition and Health
- COVID-19 Impact on Reproduction
- Bacterial Infections and Vaccines
- vaccines and immunoinformatics approaches
ETH Zurich
2021-2025
International Centre for Genetic Engineering and Biotechnology
2020
Subclinical mastitis, the inflammation of mammary gland lacking clinical symptoms, is one most prevalent and costly diseases in dairy farming worldwide. Milk microRNAs (miRNAs) encapsulated extracellular vesicles (EVs) have been proposed as potential biomarkers different conditions, including subclinical mastitis. However, little known about robustness EVs analysis regarding sampling time-point natural infections. To estimate reliability measurements raw bovine milk, we first evaluated...
Abstract Staphylococcus aureus ( S. ) is one of the most common causative agents mammary gland infection and mastitis, but specific role -derived extracellular vesicles (SaEVs) in mastitis has been poorly studied to date. Here, we aimed investigate response bovine monocyte-derived macrophages (boMdM) SaEVs genotype B (GTB) mastitis-related strain M5512B. Specifically, evaluated effects on actin cytoskeleton, gene expression, SaEV proteomic cargo. Furthermore, assessed what extent cellular...
Extracellular vesicles (EVs) and their microRNA (miRNA) cargo have been proposed as possible mammary gland health biomarkers in cattle. However, throughout the day, biologically active milk components, such miRNAs, may change due to dynamic nature of milk. The current study aimed evaluate circadian fluctuation EVs miRNA assess feasibility future for management. Milk from four healthy dairy cows was collected consecutive days two daily milking sessions morning evening. isolated were...
Milk extracellular vesicles (EVs) have gained extensive attention as promising diagnostic and therapeutic tools. Pre-analytical raw milk storage at low temperatures is an ordinary usually necessary step after sample collection. It known that direct freezing of unprocessed whole contaminates the native pool EVs with other cell structures. However, less evidence available regarding prolonged cooling 4°C. The current study assessed whether pre-analytical bovine for several days affected EV...
In dairy cows, Staphylococcus aureus (S. aureus) is among the most prevalent microorganisms worldwide, causing mastitis, an inflammation of mammary gland. Production extracellular vesicles (EVs) a common feature S. strains, which contributes to its pathogenesis by delivering bacterial effector molecules host cells. current study, we evaluated differences between five mastitis isolates regarding their EV production. We found that different mastitis-related strains differ in behaviour shedding...
Dysregulated sensing of self-nucleic acid is a leading cause autoimmunity in multifactorial and monogenic diseases. Mutations Wiskott-Aldrich syndrome protein (WASp), key regulator cytoskeletal dynamics immune cells, autoimmune manifestations increased production type I IFNs by innate cells. Here we show that complexes self-DNA autoantibodies (DNA-ICs) contribute to elevated IFN levels via activation the cGAS/STING pathway cytosolic sensing. Mechanistically, lack endosomal F-actin nucleation...
Extracellular Vesicles and Circulating Nucleic Acids is an open access journal, focusing on extracellular vesicles circulating nucleic acids including DNA, RNA, miRNA their therapeutic use.
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) induces B and T cell responses, contributing to virus neutralization. In a cohort of 2,911 young adults, we identified 65 individuals who had an asymptomatic or mildly symptomatic SARS-CoV-2 infection characterized their humoral responses the Spike (S), Nucleocapsid (N) Membrane (M) proteins. We found that previous induced CD4 cells vigorously responded pools peptides derived from S N By using statistical machine learning...
Abstract Background: Subclinical mastitis, the inflammation of mammary gland lacking clinical symptoms, is one most prevalent and costly diseases in dairy farming worldwide. Milk microRNAs (miRNAs) encapsulated extracellular vesicles (EVs) have been proposed as potential biomarkers different conditions, including subclinical mastitis. However, little known about robustness EVs analysis regarding sampling time-point or natural infections. To estimate reliability measurements raw bovine milk,...
Abstract Aim Mammary gland extracellular vesicles (EVs) are found in both human and livestock milk. Our knowledge of the role EVs mammary development, breast cancer mastitis derives mainly from vitro cell culture models. However, a commonly shared limitation is use foetal bovine serum (FBS) as supplement, which naturally contains EVs. For this reason, purpose study to establish novel tool investigate an FBS-free system. Methods Primary epithelial cells (pbMECs) alveolar line (MAC-T) were...