A5045846042- Pancreatic function and diabetes
- MicroRNA in disease regulation
- Diabetes and associated disorders
- Metabolism, Diabetes, and Cancer
- RNA Research and Splicing
- Epigenetics and DNA Methylation
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Circular RNAs in diseases
- Immune Cell Function and Interaction
- Genetic diversity and population structure
- Marine and fisheries research
- Marine and coastal plant biology
- Neuroendocrine Tumor Research Advances
- Renin-Angiotensin System Studies
- Diet, Metabolism, and Disease
- Diabetes Treatment and Management
- COVID-19 Clinical Research Studies
- Genetic Neurodegenerative Diseases
- Nitric Oxide and Endothelin Effects
- Genomics and Chromatin Dynamics
- SARS-CoV-2 and COVID-19 Research
- Species Distribution and Climate Change
- Coral and Marine Ecosystems Studies
- Diabetes Management and Research
Inspire
2022-2024
ETH Zurich
2015-2023
Board of the Swiss Federal Institutes of Technology
2015
National Institutes of Health
2013-2014
Eunice Kennedy Shriver National Institute of Child Health and Human Development
2013-2014
University of Lausanne
2013
University of California, Berkeley
2010
Expression changes of competing endogenous RNAs (ceRNAs) have been proposed to influence microRNA (miRNA) activity and thereby regulate other transcripts containing miRNA-binding sites. Here, we find that although miRNA levels define the extent repression, they little effect on magnitude ceRNA expression change required observe derepression. Canonical 6-nt sites, which typically mediate modest can nonetheless compete for binding, with potency ∼20% observed canonical 8-nt In aggregate,...
MicroRNAs (miRNAs) are important regulators of cell-autonomous gene expression that influence many biological processes. They also released from cells and present in virtually all body fluids, including blood, urine, saliva, sweat, milk. The functional role nutritionally obtained extracellular miRNAs is controversial, irrefutable demonstration exogenous miRNA uptake by canonical function still lacking. Here we show at high levels the milk lactating mice. To investigate intestinal newborn...
The epithelial-to-mesenchymal transition (EMT) is an important mechanism for cancer progression and metastasis. Numerous in vitro tumor-profiling studies point to the miR-200-Zeb1 axis as crucial regulating this process, yet vivo involving its regulation within a physiological context are lacking. Here, we show that miR-200 ablation Rip-Tag2 insulinoma mouse model induces beta-cell dedifferentiation, initiates EMT expression program, promotes tumor invasion. Strikingly, disrupting sites of...
A case-control study revealed association between hypertension and rs3918226 in the endothelial nitric oxide synthase (eNOS) gene promoter (minor/major allele, T/C allele). We aimed at substantiating these preliminary findings by target sequencing, cell experiments, a population study. sequenced 140-kb genomic area encompassing eNOS gene. In HeLa HEK293T cells transfected with carrying either T or C we quantified transcription luciferase assay. 2722 randomly recruited Europeans (53.0% women;...
Charcot–Marie–Tooth disease (CMT) comprises a clinically and genetically heterogeneous group of peripheral neuropathies characterized by progressive distal muscle weakness atrophy, foot deformities sensory loss. Following the analysis two consanguineous families affected medium to late-onset recessive form intermediate CMT, we identified overlapping regions homozygosity on chromosome 1p36 with combined maximum LOD score 5.4. Molecular investigation genes from this region allowed...
The miR-200–Zeb1 axis regulates the epithelial-to-mesenchymal transition (EMT), differentiation, and resistance to apoptosis. A better understanding of these processes in diabetes is highly relevant, as β-cell dedifferentiation apoptosis contribute loss functional mass progression. Furthermore, EMT promotes identity vitro expansion human islets. Though miR-200 family has previously been identified a regulator vivo, studies focusing on Zeb1 are lacking. aim this study was thus investigate...
MicroRNAs (miRNAs) are small non-coding RNAs that regulate the expression of their target genes at post-transcriptional level. In cancer cells, miRNAs, depending on biological functions genes, may have a tumor-promoting or -suppressing effect. Treatment cells with inhibitors DNA methylation and/or histone deacetylation modulates level which provides evidence for epigenetic regulation miRNA expression. The consequences inhibition methyltransferase expression, however, not been thoroughly...
Western burrowing owls (Athene cunicularia hypugaea) are ground-dwelling distributed throughout western North America. Because of population declines, this species is considered endangered in Canada, and listed as a conservation concern states the USA. Korfanta et al. (2002) previously presented primers for seven microsatellite loci owls. Parentage relatedness studies require larger number markers accuracy precision. Here, we developed characterized 18 additional DNA loci, tested these 23...
Abstract Aims/hypothesis In type 1 diabetes, the counterregulatory glucagon response to low plasma glucose is impaired. The resulting increased risk of hypoglycaemia necessitates novel strategies ameliorate alpha-cell impairment. Here, we aimed establish an in vitro model impairment diabetes using human islet microtissues (MTs) exposed proinflammatory cytokines. Additionally, investigated therapeutic potential incretin receptor agonists improving responses glucose. Methods Human MTs were...
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) induces B and T cell responses, contributing to virus neutralization. In a cohort of 2,911 young adults, we identified 65 individuals who had an asymptomatic or mildly symptomatic SARS-CoV-2 infection characterized their humoral responses the Spike (S), Nucleocapsid (N) Membrane (M) proteins. We found that previous induced CD4 cells vigorously responded pools peptides derived from S N By using statistical machine learning...
Abstract The pro-inflammatory cytokines IFNα, IFNγ, IL-1β and TNFα may contribute to innate adaptive immune responses during islet inflammation (insulitis) in type 1 diabetes (T1D). We used deep RNA-sequencing analysis characterize the response of human pancreatic beta cells each cytokine individually compared signatures obtained with those present islets individuals affected by T1D. IFNα IFNγ had a much greater impact on cell transcriptome when TNFα. IFN-induced gene have strong correlation...
The RNA‐binding protein Lin28a is considered to be a “stemness” marker. expressed predominantly in pluripotent cells and cancer cells. However, the regulation of its expression has not been thoroughly studied. We found that promoter DNA was transcriptionally active non ‐expressing cells, which made us hypothesize regulated epigenetically. By bisulfite‐sequencing analysis, we did observe correlation between absence gene methylation. Consistently, activated non‐ expressing by...