Human fibrinogen cDNA probes for the alpha-, beta-, and gamma-polypeptide chains have been used to isolate corresponding genes from human genomic libraries. There is a single copy of each gene. Restriction endonuclease analysis isolated clones DNA indicates that gamma-fibrinogen are closely linked in 50-kilobase region chromosome: alpha-gene middle flanked by beta-gene on one side gamma-gene other. The alpha- gamma-chain oriented tandem transcribed toward beta-chain gene opposite strand...

The c-myb protooncogene encodes proteins that are critical for hematopoietic cell proliferation and development. Disrupting function might, therefore, prove an effective therapeutic strategy controlling leukemic growth. Antisense oligodeoxynucleotides have been utilized this purpose in vitro, but their vivo efficacy has not reported. We therefore established human leukemia-scid mouse chimeras with K562 cells treated diseased animals phosphorothioate-modified antisense oligodeoxynucleotides....

The chromatin structure of the interleukin-2 (IL-2) gene was probed by DNase I treatment isolated nuclei. 5' region IL-2 contains three regions hypersensitivity to I. When peripheral blood T cells or Jurkat are stimulated with mitogens, message is induced, and promoter develops an additional hypersensitive site. This suggests that a DNA sequence close transcriptional start site involved in transduction extracellular signal. Such conclusion further supported transfection experiments. A short...

Genital infections due to herpes simplex virus type 2 (HSV-2) are characterized by frequent reactivation and shedding of the attendant risk transmission sexual partners. We investigated effects vaginal coinfections hormonal contraceptive use on genital tract HSV-2 in women.A total 330 HSV-2-seropositive women were followed every 4 months for a year. At each visit, one swab specimen was obtained detection polymerase chain reaction, second group B Streptococcus (GBS) organisms yeast culture,...

Recently, we have found that defibrination of rats with Malayan pit viper venom induces a 10-38-fold increase in the levels translatable fibrinogen mRNA liver.We used this response to obtain cDNA clones for three polypeptide chains rat fibrinogen.A large library was created pBR322 from induced liver polyadenylated RNA by poly(dG, dC)-tailing method.Part screened using colony hybridization [32P]cDNA prepared and noninduced RNA.Colonies consistently giving more intense signal were considered...

BALB/c mice were inoculated subcutaneously with 10(6) cells from either of two syngeneic sarcomas 1315 and 1425. 6--8 days later, the randomized into groups which left untreated or given 400 rads whole body irradiation. Irradiation significantly retarded growth both sarcomas, complete regressions seen approximately equal to 30% small, established tumors. The anti-tumor effect irradiation was abolished if irradiated a T-cell-enriched (but not T-cell deprived) suspension spleen cells,...

The y chain of human fibrinogen exists in 2 nonallelic forms, yA and yB, which differ only their carboxyl termini.We have found that one genomic locus for y-fibrinogen the yB chains arise by alternate mRNA splicing near 3' end this gene.In contrast to rat rB is ' abbreviations used are: bp, base pair(s); kb, kilobase pairs.12826

Using rat cDNA and genomic probes to screen a human liver library, we have isolated clone of 2,274, 855, 736 base pairs (bp) coding for the A alpha, B beta gamma chains fibrinogen. Sequence analysis reveals hitherto unrecognized extension 15 amino acids at carboxyl terminus alpha chain, terminal residue which is proline. This brings known length chain 625 acids. The 13-amino-acid repeated region in midportion clearly has arisen through an 8-fold duplication 39-bp genetic element, itself...

Cell-free translation and hybridization to cloned cDNA probes were used study variations in fibrinogen mRNA levels the livers of rats injected with Malayan pit viper venom glucocorticoids.Animals defibrinated showed a rapid substantial increase relative abundance hepatic mRNAs for Aa, BP, y chains fibrinogen.The onset, rate, maximal extent message accumulation virtually identical each three polypeptide chains.This response was detectable 1-2 h after injection and, by 12-16 h, species made up...

Cow's milk allergy is one of the most common food allergies in children and no treatment available. Dietary lipid composition may affect susceptibility to develop allergic disease.Assess whether dietary supplementation with long chain n-3 polyunsaturated fatty acids (n-3 LCPUFA) prevents establishment allergy.Mice were fed a control or fish oil diet before during oral sensitization whey. Acute skin response, serum immunoglobulins as well dendritic cell (DC) T subsets mesenteric lymph nodes...

Vascular access failure is not only a big challenge for nephrologists but also threat patients on haemodialysis. Due to various causes, hemodialysis land eventually at this dead end. Patients who have undergone multiple surgeries intrabdominal adhesions which makes peritoneal dialysis impossible. Such two options; translumbar and transhepatic access. We report case of 54-year-old woman had vascular failure. She was obese apprehensive about the approach tunneled catheter placement. The...

The prevalence of gallbladder stones is higher in Chronic Kidney Disease (CKD) patients and it has been shown to increase with the advancement disease stage, from 7.7% stage 1% 21.3% 5. Gallstone ileus a rare complication which presents just 0.3% - 0.5% cholelithiasis. A 61-year-old female patient, known case CKD on maintenance hemodialysis, (thrice week) primary hypertensive diabetic nephropathy; presented multiple episodes loose stool, vomiting, diffuse abdominal pain for 2 days. Abdomen...

We investigated the therapeutic potential of employing antisense oligodeoxynucleotides to target disruption MYB, a gene which has been postulated play pathogenetic role in cutaneous melanoma. found that MYB was expressed at low levels several human melanoma cell lines. Also, growth representative lines vitro inhibited dose- and sequence-dependent manner by targeting with unmodified or phosphorothioate-modified oligodeoxynucleotides. Inhibition correlated specific decrease mRNA. In SCID mice...

ABSTRACT Knowledge of polyomavirus BK (BKV) genomic diversity has greatly expanded. The implications BKV DNA sequence variation for the performance molecular diagnostic assays is not well studied. We analyzed 184 publically available VP-1 sequences encompassing region targeted by an in-house quantitative hydrolysis probe-based PCR assay. A perfect match with primers and probe was seen in 81 sequences. One Dun 13 variant prototype oligonucleotides were synthesized as artificial targets to...

Non-Hodgkin lymphoma is a common feature of AIDS. Approximately 30-40% these tumors exhibit clinical features suggestive endemic Burkitt lymphoma: they are aggressive malignancies that occur in association with Epstein-Barr virus infection, arise the setting immunosuppression, and carry t(8;14) translocations without detectable rearrangement MYC oncogene. To understand molecular basis parallels, we analyzed case Epstein-Barr-positive AIDS-associated undifferentiated lymphoma. Southern blots...

We have examined regulatory domains of the human IL-2 gene promoter by transfection and transient expression rDNA constructs in which chloramphenicol acetyl transferase shows T cell-specific inducible cyclosporin A-mediated inhibition when placed downstream 587 bp 5'-flanking region. A series 5'-deletion transfected into Jurkat lymphoid line demonstrates that a region encompassing 370 5' transcription start site is sufficient for acetyl-transferase expression. Further dissection this with...

Prostate cancer (PCa) is a highly prevalent type with heterogeneous prognosis. An accurate assessment of tumor aggressiveness can pave the way for tailored treatment strategies, potentially leading to better outcomes. While typically assessed based on invasive methods (e.g., biopsy), radiogenomics, combining diagnostic imaging genomic information help uncover aggressive (imaging) phenotypes, which in turn provide non-invasive advice individualized regimens. In this study, we carried out...

The congenital form of myotonic dystrophy is reported to be almost exclusively, if not maternally transmitted. We present a case which was inherited from mildly affected father. This family illustrates that the can occur without intrauterine or other maternal factors related disease. possibility paternal transmission could considered when counselling patients and their families.

CD28 is a glycoprotein expressed as homodimer on the surface of major subset human T cells. Previous studies have shown that proliferation peripheral blood cells involving pathway associated with cyclosporine A (CsA) resistant IL-2 gene expression. This was to specifically regulate stability mRNA for several lymphokines including IL-2. We investigated expression in Jurkat cell line, J32 clone, induced by stimulation. Cross-linked anti-CD28 mAb alone sufficient induce release small amounts...

We have utilized cDNA probes for the a, p, and y chains of rat fibrinogen to isolate corresponding genes from two genomic libraries constructed in bacteriophage Charon 4A.There is a single copy each gene.Mapping greater than 92 kilobase pairs DNA has shown that chain are directly linked 5'-3' direction vivo.Genes number mammalian multigenic families been be clustered small regions chromosome.The best studied examples include / 3 globin polypeptides (1) constant immunoglobulin light (2-4)...

Abstract Data on polyomavirus genomic diversity has greatly expanded in the past few years. The implications of viral DNA sequence variation performance molecular diagnostic assays have not been systematically examined. 716 BK, 1626 JC, and 73 SV40 virus sequences available GenBank were aligned using Clustal‐X. Five different published BKV PCR currently use at major medical centers evaluated for primer probe mismatches with sequences. Coverage naturally occurring strains varied amongst assay...

Recent studies have shown that antibodies to certain epitopes on the 50-kd molecule associated with sheep erythrocyte receptors human T cells can suppress cell proliferation and interleukin 2 (IL 2) elaboration. We used a IL cDNA clone investigate effect of antibody 9.6 cyclosporin A (CsA) regulation mRNA levels in cloned leukemic line Jurkat, J32. Maximal were reached 6 hr after induction Jurkat combination mitogen phytohemagglutinin (PHA) phorbol ester (TPA). Antibody 9.6, added during...