Abstract Osteoarthritis (OA) is a heterogeneous disease that extremely hard to cure owing its complex regulation network of pathogenesis, especially cartilage degeneration. FBXO21 subunit ubiquitin E3 ligases degrades P‐glycoprotein and EID1 by ubiquitination activates the JNK p38 pathways; however, role in OA remains unknown. Here, main objective this study was evaluate potential effects mechanism degeneration, we revealed upregulated patients with OA, aging, monosodium iodoacetate‐induced...

Osteoarthritis (OA) is a common joint disease, and studies have reported that the endoplasmic reticulum stress (ERS) in chondrocytes caused by cartilage tissue damage could mediate activation of Nod-like receptor protein 3 (NLRP3) inflammasomes through inositol-requiring enzyme 1 alpha (IRE1α) thioredoxin interacting (TXNIP). Ginsenoside compound K (CK) has an inhibitory effect on IRE1α activation. However, role IRE1α-TXNIP its interaction with CK are still unclear. In this study, we...

Aim: Maresin-1 is a metabolite of docosahexaenoic acid (DHA) that has potential anti-inflammatory effects. To explore whether maresin-1 changes and therapeutic effect in osteoarthritis (OA) model rats undergoing treadmill exercise, we examined endogenous single-session experiment OA were treated with maresin-1, moreover, the effects on IL-1β induced rat fibroblast-like synoviocytes (FLSs) possible mechanisms.Methods: In experiment, 48 randomly divided into 3 groups performed three different...

Protectin DX (PDX) has been reported to have extensive anti-inflammatory effects. However, it is unknown whether PDX acts as an agent in the context of osteoarthritis (OA). This study aimed evaluate activity vitro and vivo a model OA. Primary rat chondrocytes were preincubated with 1 h prior IL-1β treatment for 24 h. We found that was nontoxic, pretreatment increased cell viability IL-1β-induced chondrocytes. Preincubation also efficiently inhibited degradation type II collagen...

To explore the effect of sub-minimal inhibitory concentration (sub-MIC) and concentrations within resistant mutation window (MSW) ciprofloxacin (CIP) on minimal (MIC), swimming motility biofilm formation Pseudomonas aeruginosa, also to investigate correlation between genes expression lasI, lasR, rhlI, rhlR pqsR. The collected strains were incubated under four different for 5 days. MIC mutant prevention (MPC) measured by agar dilution method. diameter turbid cycle was used signify motility....

To investigate the main molecular resistance mechanisms to fluoroquinolones (FQs) in Pseudomonas aeruginosa and also effect of time concentration on mutations genes. The clinical isolates P. which are sensitive ciprofloxacin (CIP) or levofloxacin (LEV) were collected. incubated with different concentrations CIP LEV for 5 days minimal inhibitory (MICs) CIP, ofloxacin (OFX) measured. MIC FQs was measured by agar dilution method. FQ determining regions gyrA, gyrB, parC parE amplified PCR, four...

The aim of this study was to examine exercise-related genes in articular cartilage identified through bioinformatics analysis dissect the potential signaling pathway involved mechanical stimulation osteoarthritis (OA). To end, we evaluated GSE74898 dataset from Gene Expression Omnibus database for differentially expressed miRNAs (DE-miRNAs) using R software package and predicted target these miRTarBase. Functional annotation enrichment were performed DE-miRNA targets. effects on tumor...

Diabetes and its complications are serious medical global burdens, often manifesting as postprandial hyperglycemia. In recent years, considerable research attention has focused on relationships between the gut microbiota circulating glucose (PPG). Different population studies have suggested that PPG is closely related to which may impact via short-chain fatty acids (SCFAs), bile (BAs) trimethylamine N-oxide (TMAO). Studies now show models can predict PPG, with individualized nutrition...

Osteoarthritis (OA) is a chronic progressive degenerative joint disease. Owing to its complex pathogenesis, OA treatment typically challenging. Necroptosis form of programmed cell death mainly mediated by the serine/threonine kinases, RIPK1 and RIPK3, mixed lineage kinase-like domain (MLKL). In this study, we found that multi-targeted kinase inhibitor DCC-2036 can inhibit TSZ (TNF-α, Smac mimetic, z-VAD-FMK)-induced necroptosis chondrocytes synovial fibroblast cells (SFs). addition, oral...

Osteoarthritis (OA), a degenerative joint disease, involves synovial inflammation, subchondral bone erosion, and cartilage degeneration. Ferroptosis, regulated non-apoptotic programmed cell death, is associated with various diseases. This study investigates ferroptosis-related molecular subtypes in OA to comprehend underlying mechanisms. The Gene Expression Omnibus datasets GSE206848, GSE55457, GSE55235, GSE77298 GSE82107 were used utilized. Unsupervised clustering identified the gene (FRG)...

Introduction Osteoarthritis (OA) is a prevalent chronic degenerative disease, marked by complex interplay of mechanical stress, inflammation, and metabolic imbalances. Recent studies have highlighted the potential spermidine (SPD), naturally occurring polyamine known for its anti-inflammatory antioxidant properties, as promising therapeutic agent OA. This study delves into efficacy mechanistic pathways SPD in mitigating OA symptoms. Methods Forty Sprague-Dawley rats were randomly assigned to...

Abstract The coronavirus disease 2019 (COVID-19) pandemic has brought severe challenges to global public health. Many studies have shown that obesity plays a vital role in the occurrence and development of COVID-19. Obesity exacerbates COVID-19, leading increased intensive care unit hospitalization rate, high demand for invasive mechanical ventilation, mortality. mechanisms interaction between COVID-19 involve inflammation, immune response, changes pulmonary dynamics, disruptions receptor...

Aim: This study investigated the association between voriconazole-induced liver injury and gene polymorphisms of CYP2C19 UGT1A4. Materials & methods: Thirty-eight adult patients who received voriconazole therapy were included in study. Genotype was detected using chip hybrid analysis. The UGT1A4 142T>G genotyped PCR-RFLP Results: Ten (26.3%) had considered as case group There no significant difference two groups genotype allele frequencies CYP2C19*2 (p > 0.05), however, GA frequency *3...

Abstract Purpose To design a new type of screw for minimally invasive atlantoaxial anterior transarticular (AATS) fixation with diameter that is significantly thicker than traditional screws, threaded structures at both ends, and porous metal structure in the middle. The use can effectively promote bone fusion compensate disadvantages AATSs terms insufficient strength difficulty fusion. biomechanical stability this was verified through finite element analysis. This instrument may provide...

The surface conditions in the northern slope area of Zhahaquan Qaidam Basin are complex, and underground is affected by tectonic extrusion movements, resulting development faults fractures. existing six blocks 3D seismic data this have a significant time span acquisition varying quality. single-block processing results indicate low signal-to-noise ratio connection area, along with substantial differences infrequency, phase, energy. fracture imaging poor, making it challenging to accurately...

Static correction is a core of data processing in complex rugged topography areas and decisive to result. There are many disadvantages conventional near surface modeling method, which cannot give high precision velocity model weathering layer especially areas, such as mountains, deserts, etc. So new mode proposed inverse short refraction first break time based on tomographic theory, then the information within array length got. It overcomes shortcomings interpretation increases precision....

Abstract Purpose: To design a new type of screw for minimally invasive atlantoaxial anterior transarticular (AATS) fixation with diameter that is significantly thicker than traditional screws, threaded structures at both ends, and porous metal structure in the middle. The use can effectively promote bone fusion compensate disadvantages AATSs terms insufficient strength difficulty fusion. biomechanical stability this was verified through finite element analysis. This instrument may provide...

Abstract Purpose : To design a new type of screw for minimally invasive atlantoaxial anterior transarticular (AATS) fixation with diameter that is significantly thicker than traditional screws, threaded structures at both ends, and porous metal structure in the middle. The use can effectively promote bone fusion compensate disadvantages AATSs terms insufficient strength difficulty fusion. biomechanical stability this was verified through finite element analysis. This instrument may provide...

Abstract Purpose: To design a new type of screw for minimally invasive atlantoaxial anterior transarticular (AATS) fixation with diameter that is significantly thicker than traditional screws, threaded structures at both ends, and porous metal structure in the middle. The use can effectively promote bone fusion compensate disadvantages AATSs terms insufficient strength difficulty fusion. biomechanical stability this was verified through finite element analysis. This instrument may provide...

Background: Osteoarthritis (OA) is a chronic progressive degenerative joint disease. Owing to the complex pathogenesis, treatment of OA typically challenging. Necroptosis form programmed cell death mediated by serine/threonine kinase, RIPK1 and RIPK3, MLKL, which could be potential option.Methods: The effects Bcr-Abl inhibitor DCC-2036 on rat articular chondrocyte cells were examined in vitro vivo. We investigated molecular mechanism drug identified target DCC-2036.Findings: In this study,...