A5103857771- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- Viral Infectious Diseases and Gene Expression in Insects
- RNA regulation and disease
- Plant Gene Expression Analysis
- Pluripotent Stem Cells Research
- Advanced biosensing and bioanalysis techniques
- Plant tissue culture and regeneration
- RNA and protein synthesis mechanisms
- Horticultural and Viticultural Research
- Plant Physiology and Cultivation Studies
- Plant Reproductive Biology
- Mathematical and Theoretical Epidemiology and Ecology Models
- Plant Stress Responses and Tolerance
- Reproductive Biology and Fertility
- Inflammasome and immune disorders
- Protein Degradation and Inhibitors
- Genomics, phytochemicals, and oxidative stress
- Opinion Dynamics and Social Influence
- Insect symbiosis and bacterial influences
- Human Pose and Action Recognition
- Animal Virus Infections Studies
- Muscle Physiology and Disorders
Guizhou University
2009-2025
Guangzhou Institutes of Biomedicine and Health
2016-2024
Guangzhou Medical University
2017-2024
Hangzhou Dianzi University
2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2022-2023
Sanya University
2022-2023
Chinese Academy of Sciences
2016-2022
University of Chinese Academy of Sciences
2017-2021
China Agricultural University
2021
Anhui Agricultural University
2019
Abstract Cytosine base editors (CBEs) enable programmable C-to-T conversion without DNA double-stranded breaks and homology-directed repair in a variety of organisms, which exhibit great potential for agricultural biomedical applications. However, all reported cases only involved substitution at single targeted genomic site. Whether is effective multiple sites/loci has not been verified large animals. Here, by using pigs, an important animal agriculture biomedicine, as the subjective animal,...
Despite being time-consuming and costly, generating genome-edited pigs holds great promise for agricultural, biomedical, pharmaceutical applications. To further facilitate genome editing in pigs, we report here establishment of a pig line with Cre-inducible Cas9 expression that allows variety ex vivo fibroblast cells including single- multigene modifications, chromosome rearrangements, efficient genetic modifications. As proof principle, were able to simultaneously inactivate five tumor...
Abstract Background Many favorable traits of crops and livestock human genetic diseases arise from multiple single nucleotide polymorphisms or point mutations with heterogeneous base substitutions at the same locus. Current cytosine adenine editors can only accomplish C-to-T (G-to-A) A-to-G (T-to-C) in windows target genomic sites organisms; therefore, there is a need to develop that simultaneously achieve targeting site. Results In this study, novel fusion editor (ACBE) was generated by...
Pig cloning by somatic cell nuclear transfer (SCNT) remains extremely inefficient, and many cloned embryos undergo abnormal development. Here, profiling transcriptome expression, we observed dysregulated chromosome-wide gene expression in every chromosome identified a considerable number of genes that are aberrantly expressed the embryos. In particular, XIST, long non-coding RNA gene, showed high ectopic We also proved nullification XIST donor cells can normalize aberrant enhance long-term...
The Bone Morphogenetic Protein 15 (BMP15) gene is known to have multiple single-nucleotide polymorphism sites associated with sheep fecundity. This study used sequence analysis and mutation detection assays for BMP15 by using 205 blood samples of ewes lambing records. Sequence showed that B1 missed the CTT base in exon 1 at positions 28–30, leading a leucine deletion protein. Litter size differed significantly between BB B+ genotypes (p < 0.05); however, differences wild genotype (++)...
Abstract The CRISPR/Cas9 system has shown great potential for treating human genetic diseases through gene therapy. However, there are concerns about the safety of this system, specifically related to use guide-free Cas9. Previous studies have that Cas9 can induce genomic instability in vitro. vivo risks associated with not been evaluated, which is necessary development therapy clinical settings. In study, we used doxycycline-inducible Cas9-expressing pigs evaluate vivo. Our findings...
Porcine deltacoronavirus (PDCoV), an emerging enteropathogenic coronavirus, not only causes diarrhea in piglets but also possesses the potential to infect humans. To better understand host-virus genetic dependencies and find therapeutic targets for PDCoV, we used a porcine single-guide RNA (sgRNA) lentivirus library screen host factors related PDCoV infection LLC-PK1 cells. The solute carrier family 35 member A1 (SLC35A1), key molecule sialic acid (SA) synthesis pathway, was identified as...
Phenylalanine ammonia lyase (PAL) plays an important role in the biosynthesis of secondary metabolites regulating plant growth response. To date, evolutionary history PAL family Rosaceae plants remains unclear. In this study, we identified 16 homologous genes five ( Pyrus bretschneideri , Fragaria vesca Prunus mume persica and Malus × domestica ). We classified these s into three categories based on phylogenetic analysis, all were distributed 13 chromosomes. tracked gene duplication events...
Abstract Cas12a can process multiple sgRNAs from a single transcript of CRISPR array, conferring advantages in multiplexed base editing when incorporated into editor systems, which is extremely helpful given that phenotypes commonly involve genes or single-nucleotide variants. However, through Cas12a-derived editors has been barely reported, mainly due to the compromised efficiencies and restricted protospacer-adjacent motif (PAM) TTTV for wild-type Cas12a. Here, we develop Cas12a-mediated...
CRISPR-based toolkits have dramatically increased the ease of genome and epigenome editing. SpCas9 is most widely used nuclease. However, difficulty delivering inability to modulate its expression in vivo hinder widespread adoption large animals.
Patients with hereditary tyrosinemia type I (HT1) present acute and irreversible liver kidney damage during infancy. CRISPR-Cas9-mediated gene correction infancy may provide a promising approach to treat patients HT1. However, all previous studies were performed on adult HT1 rodent models, which cannot authentically recapitulate some symptoms of human patients. The efficacy safety should be verified in large animals translate precise therapy clinical practice. Here, we delivered CRISPR-Cas9...
Abstract Background Prime editing enables precise base substitutions, insertions, and deletions at targeted sites without the involvement of double-strand DNA breaks or exogenous donor templates. However, large size prime editors (PEs) hampers their delivery in vivo via adeno-associated virus (AAV) due to viral packaging limit. Previously reported split PE versions provide a reduction, but they require intricate engineering potentially compromise efficiency. Results Herein, we present...
Abstract Background Mutations in the DMD gene encoding dystrophin—a critical structural element muscle cells—cause Duchenne muscular dystrophy (DMD), which is most common fatal genetic disease. Clustered regularly interspaced short palindromic repeat (CRISPR)-mediated editing a promising strategy for permanently curing DMD. Methods In this study, we developed novel reframing mutations via CRISPR-mediated large-scale excision of exons 46–54. We compared approach with other rescue strategies...
Many efforts have been made to improve the precision of Cas9-mediated gene editing through increasing knock-in efficiency and decreasing byproducts, which proved be challenging.
The NLRP3 inflammasome is associated with a variety of human diseases, including cryopyrin-associated periodic syndrome (CAPS). CAPS dominantly inherited disease missense mutations. Currently, most studies on the NLRP3-inflammasome have been performed mice, but activation patterns and signaling pathways mouse are not always identical those in humans. pigs similar to that Therefore, precise NLRP3-point mutations may model more accurately. In this study, an gain-of-function pig carrying...
Abstract Conditional gene regulation systems can control expression in predefined tissues or organs at a desired time. Site‐specific recombinase and chemically induced are the two most widely used approaches for creating genetically modified (GM) animals with conditional of expression. Generation GM pigs controllable elements, usually involving multiple editing, to be major challenge due lack germ line‐competent pluripotent stem cells. With emergence artificial endonuclease‐mediated editors,...
To probe into the internal mechanism of multi-agent formation, game theory is used to model interaction between agents and Win-Stay-Lose-Shift strategy instruct agents' action. Equations are introduced formulate how update their positions. The along with equations depicts dynamics formation. And simulations designed performed observe development results simulation show feasibility idea in this paper.