A5053689344- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Viral Infections and Immunology Research
- interferon and immune responses
- SARS-CoV-2 and COVID-19 Research
- RNA regulation and disease
- RNA Research and Splicing
- Plant Virus Research Studies
- RNA Interference and Gene Delivery
- HIV Research and Treatment
- Protein Degradation and Inhibitors
- Endoplasmic Reticulum Stress and Disease
- Selenium in Biological Systems
- Histone Deacetylase Inhibitors Research
- Cancer Research and Treatments
- Mitochondrial Function and Pathology
- Digestive system and related health
- Liver Disease Diagnosis and Treatment
- Heat shock proteins research
- Cancer, Hypoxia, and Metabolism
- Extracellular vesicles in disease
- Nanocluster Synthesis and Applications
- Cancer Cells and Metastasis
- Cytomegalovirus and herpesvirus research
Shandong Agricultural University
2024-2025
Center of Hubei Cooperative Innovation for Emissions Trading System
2016-2024
Huazhong Agricultural University
2016-2024
Nanjing Agricultural University
2014
ABSTRACT Porcine deltacoronavirus (PDCoV) has recently emerged as an enteric pathogen that can cause serious vomiting and diarrhea in suckling piglets. The first outbreak of PDCoV occurred the United States 2014 was followed by reports South Korea, China, Thailand, Lao People's Democratic Republic, Vietnam, leading to economic losses for pig farms posing a considerable threat swine industry worldwide. Our previous studies have shown encodes three accessory proteins, NS6, NS7, NS7a, but...
Porcine reproductive and respiratory syndrome virus (PRRSV) is a typical immunosuppressive devastating the global swine industry. DEAD-box helicases (DDXs) are family of ATP-dependent RNA that predominantly implicated in modulating cellular metabolism. Meanwhile, growing number studies have suggested some DDXs associated with innate immunity infection, so they considered potential antiviral targets. Herein, we screened 40 found ectopic expression DDX10 exhibited significant anti-PRRSV...
Porcine deltacoronavirus (PDCoV) is an emerging swine enteric coronavirus. Bioinformatics predicts that PDCoV encodes two accessory proteins (NS6 and NS7), the species-specific for In this study, four mAbs against predicted NS7 were prepared by using purified recombinant protein. Indirect immunofluorescence assay demonstrated all recognized cells transfected with expression construct or infected PDCoV. Western blot showed NS7-specific additional protein band of about 12 kDa from...
HDAC6, a structurally and functionally unique member of the histone deacetylase (HDAC) family, is an important host factor that restricts viral infection. The broad-spectrum antiviral activity HDAC6 makes it potent agent. Previously, we found functions to antagonize porcine deltacoronavirus (PDCoV), emerging enteropathogenic coronavirus with zoonotic potential. However, final outcome typically productive infection materializes as cells succumb infection, indicating virus has evolved...
G-quadruplex (G4) is a unique secondary structure formed by guanine-rich nucleic acid sequences. Growing studies reported that the genomes of some viruses harbor G4 structures associated with viral replication, opening up new field to dissect infection. Porcine reproductive and respiratory syndrome virus (PRRSV), representative member Arteriviridae, an economically significant pathogen has devastated swine industry worldwide for over 30 years. In this study, we identified highly conserved...
Porcine epidemic diarrhea virus (PEDV) is a reemerging enteropathogenic coronavirus that causes high mortality in piglets and has catastrophic effects on the global pig industry. PEDV-encoded nonstructural protein 7 (nsp7) an important component of viral replication transcription complex, previous study reported it inhibits poly(I:C)-induced type I interferon (IFN) production, but mechanism by which this occurs remains unclear. Here, we demonstrated ectopic expression PEDV nsp7 antagonized...
ABSTRACT The microtubule (MT) is a highly dynamic polymer that functions in various cellular processes through MT hyperacetylation. Thus, many viruses have evolved mechanisms to hijack the network of cytoskeleton allow intracellular replication viral genomic material. Coronavirus non-structural protein 8 (nsp8), component transcriptional complex, essential for survival. Here, we found nsp8 porcine deltacoronavirus (PDCoV), an emerging enteropathogenic coronavirus with zoonotic potential,...
Porcine deltacoronavirus (PDCoV), an emerging enteropathogenic coronavirus, not only causes diarrhea in piglets but also possesses the potential to infect humans. To better understand host-virus genetic dependencies and find therapeutic targets for PDCoV, we used a porcine single-guide RNA (sgRNA) lentivirus library screen host factors related PDCoV infection LLC-PK1 cells. The solute carrier family 35 member A1 (SLC35A1), key molecule sialic acid (SA) synthesis pathway, was identified as...
Porcine deltacoronavirus (PDCoV) is an emerging swine enteropathogenic coronavirus that causes watery diarrhea, vomiting and mortality in newborn piglets. Previous studies have suggested PDCoV infection antagonizes RIG-I-like receptor (RLR)-mediated IFN-β production to evade host innate immune defense, PDCoV-encoded nonstructural protein nsp5 accessory NS6 are associated with this process. However, whether the structural protein(s) of also antagonize remains unclear. In study, we found...
As an emerging swine enteropathogenic coronavirus, porcine deltacoronavirus (PDCoV) not only causes serious diarrhea in suckling piglets, but also possesses the potential for cross-species transmission, which has sparked growing interest studying this virus. We previously identified a novel accessory protein NS7a encoded by PDCoV; however, function of was resolved. In study, we demonstrated that PDCoV is interferon antagonist. Overexpression notably inhibited Sendai virus (SeV)-induced...
Porcine deltacoronavirus (PDCoV) is an emerging swine enteropathogenic coronavirus that has the potential to infect humans. Histone deacetylase 6 (HDAC6) a unique type IIb cytoplasmic with both activity and ubiquitin E3 ligase activity, which mediates variety of cellular processes by deacetylating histone nonhistone substrates. In this study, we found ectopic expression HDAC6 significantly inhibited PDCoV replication, while reverse effects could be observed after treatment HDAC6-specific...
The Warburg effect, also referred as aerobic glycolysis, is a common metabolic program during viral infection. Through targeted metabolomics combined with biochemical experiments and various cell models, we investigated the central carbon metabolism (CCM) profiles of cells infected porcine deltacoronavirus (PDCoV), an emerging enteropathogenic coronavirus zoonotic potential. We found that PDCoV infection required glycolysis but decreased glycolytic flux, exhibiting non-Warburg effect...
The porcine reproductive and respiratory syndrome virus (PRRSV) remains a persistent hazard in the global pig industry. DEAD (Glu-Asp-Ala-Glu) box helicase 21 (DDX21) is member of DDX family. In addition to its function regulating cellular RNA metabolism, DDX21 also regulates innate immunity involved replication cycle some viruses. However, relationship between PRRSV has not yet been explored. Here, we found that overexpression promoted replication, whereas knockdown reduced proliferation....
Porcine epidemic diarrhea virus (PEDV) is a re-emerging enteropathogenic coronavirus that causes high mortality in neonatal piglets. The addition of trypsin plays crucial role the propagation PEDV, but also increases complexity vaccine production and its cost. Previous studies have suggested S2' site Y976/977 PEDV spike (S) protein might be determinants independence. In this study, to achieve recombinant trypsin-independent strain, we used trypsin-dependent genotype 2 (G2) variant AJ1102...
ABSTRACTPorcine deltacoronavirus (PDCoV) is an emerging enteric coronavirus that has been reported to infect a variety of animals and even humans. Cell-cell fusion identified as alternative pathway for the cell-to-cell transmission certain viruses, but ability PDCoV exploit this model, relevant mechanisms, have not fully elucidated. Herein, we provide evidence main mechanism supporting spread in cell culture efficient model mediated by spike glycoprotein-driven cell-cell fusion. We found...