A5100441827- Urinary Bladder and Prostate Research
- Glutathione Transferases and Polymorphisms
- Receptor Mechanisms and Signaling
- Chemical Synthesis and Analysis
- Pelvic floor disorders treatments
- Vector-borne infectious diseases
- Periodontal Regeneration and Treatments
- HIV Research and Treatment
- Phosphodiesterase function and regulation
- Urological Disorders and Treatments
- Genomics, phytochemicals, and oxidative stress
- Ion channel regulation and function
- Pharmacogenetics and Drug Metabolism
- Neuroscience and Neuropharmacology Research
- Vector-Borne Animal Diseases
- Enzyme Catalysis and Immobilization
- Viral Infections and Vectors
- Plant tissue culture and regeneration
- Prostate Cancer Diagnosis and Treatment
- Chromosomal and Genetic Variations
- Biopolymer Synthesis and Applications
- Cancer therapeutics and mechanisms
- Biochemical and Molecular Research
- Carbohydrate Chemistry and Synthesis
- Monoclonal and Polyclonal Antibodies Research
Longhua Hospital Shanghai University of Traditional Chinese Medicine
2025
Shanghai University of Traditional Chinese Medicine
2025
Zhangqiu City People's Hospital
2025
Affiliated Hospital of Nantong University
2023-2024
Second Affiliated Hospital of Nantong University
2023-2024
Nantong University
2023-2024
Jiyang College of Zhejiang A&F University
2024
Xiamen Medical College
2023
Chinese Academy of Medical Sciences & Peking Union Medical College
2023
Shanghai Jiao Tong University
2023
Beyond sample curation and basic pathologic characterization, the digitized H&E-stained images of TCGA samples remain underutilized. To highlight this resource, we present mappings tumor-infiltrating lymphocytes (TILs) based on H&E from 13 tumor types. These TIL maps are derived through computational staining using a convolutional neural network trained to classify patches images. Affinity propagation revealed local spatial structure in patterns correlation with overall survival. map...
Abstract Background Dysbacteriosis of intestinal tract may cause systemic inflammation, making distant anatomical locations more susceptible to illness. Recent research has demonstrated that the microbiome can affect both prostatitis and inflammation prostate is linked cancer. It still unclear, though, whether this relationship indicates causation. We conducted a Mendelian randomization investigation on two samples fully uncover gut microbiota’s potential genetic causal role in prostatitis....
Anaplastic lymphoma kinase (ALK) is a receptor tyrosine (RTK) first discovered as the constitutively active nucleophosmin-ALK oncoprotein in anaplastic large cell lymphomas (ALCL). Full-length ALK has critical role normal development and differentiation. Activated full-length also found different malignant cancers. Nevertheless, ligand to activate remained unknown until recently, when was proposed be physiological of cytokine pleiotrophin (PTN, Ptn). However, earlier studies had demonstrated...
Functional expansion of specific tRNA synthetases in higher organisms is well documented. These additional functions may explain why dominant mutations glycyl-tRNA synthetase (GlyRS) and tyrosyl-tRNA cause Charcot-Marie-Tooth (CMT) disease, the most common heritable disease peripheral nervous system. At least 10 disease-causing mutant alleles GlyRS have been annotated. scatter broadly across primary sequence no apparent unifying connection. Here we report structure wild type a CMT-causing...
Abstract Objective: To evaluate the synergistic effect of bone morphogenetic protein 2 (BMP‐2) and vascular endothelial growth factor (VEGF) on repair defects around dental implants. Material methods: Five groups scaffold were fabricated by a freeze‐drying method, including pure chitosan/collagen scaffold; loaded with adenoviruses expressing BMP‐2, VEGF, both BMP‐2 VEGF adenovirus BMP‐2. In vitro studies examined whether marrow stromal cells responsive to these scaffolds over time. Bone...
Men who have sex with men (MSM) recently accounted for an alarmingly increasing proportion of HIV-1 transmission in China. In order to investigate the immune status as a result CRF01_AE infection and CXCR4 co-receptor usage young Shanghai-based HIV-1-infected MSM population Shanghai, 364 average age 22.7 years old, newly diagnosed between Jan 2009 Jul 2013 were analyzed CD4+T cell count, subtyping using phylogenetic analysis, viral tropism Geno2pheno webPSSM combination. A total 276...
This study is to explore the osteogenesis potential of human periodontal ligament stem cells (hPDLSCs) induced by naringin in vitro and vitro. The results confirmed that 1 μ M performs best effect a collection bone-related genes ( RUNX2 , COL1A2 OPN, OCN) had significantly higher expression levels compared control group. Furthermore, typical trabecular structure was observed vivo, surrounded large amount osteoblasts. These demonstrated naringin, at concentration M, can efficiently promote...
Abstract Engineering approaches for growth factor delivery have been considerably advanced tissue regeneration, yet most of them fail to provide a complex combination signals emulating natural healing cascade, which substantially limits their clinical successes. Herein, we aimed emulate the bone cascades by coupling processes angiogenesis and osteogenesis with hybrid dual system achieve vascularized formation. Basic fibroblast (bFGF) was loaded into methacrylate gelatin (GelMA) mimic...
The role of conformational ensembles in enzymatic reactions remains unclear. Discussion concerning "induced fit" versus "conformational selection" has, however, ignored detoxication enzymes, which exhibit catalytic promiscuity. These enzymes dominate drug metabolism and determine drug-drug interactions. enzyme glutathione transferase A1-1 (GSTA1-1), exploits a molten globule-like active site to achieve remarkable promiscuity wherein the substrate-free ensemble is broad with barrierless...
Conjugation to glutathione (GSH) by transferase A4-4 (GSTA4-4) is a major route of elimination for the lipid peroxidation product 4-hydroxynonenal (HNE), toxic compound that contributes numerous diseases. Both enantiomers HNE are presumed be toxic, and GSTA4-4 has negligible stereoselectivity toward them, despite its high catalytic chemospecificity alkenals. In contrast highly flexible, substrate promiscuous, GSTA1-1 isoform poor efficiency with HNE, been postulated rigid template...
<b><i>Objectives:</i></b> To investigate the microorganisms in expressed prostatic secretions (EPS) of both chronic prostatitis patients and normal young male adults to determine which are associated with degree intraprostatic inflammation. <b><i>Methods:</i></b> Specific polymerase chain reaction (PCR) technology was performed confirm <i>Neisseria gonorrhoeae</i>, <i>Mycobacterium tuberculosis</i>,...
This contribution describes the development of a ketoreductase enzyme over four rounds directed evolution and associated process that enabled practical, scalable to (S)-licarbazepine (eslicarbazepine).
Reversible clusters of nanobiocatalysts are developed via non-covalent interaction among enzyme-bound iron oxide magnetic nanoparticles. Dissociation the by shaking during biotransformation enables high catalytic performance, and re-clustering stopping after reaction allows for easy separation. The novel concept is demonstrated with alcohol dehydrogenase RDR enantioselective reduction 7-methoxy-2-tetralone.
The conventional analysis of enzyme evolution is to regard one single salient feature as a measure fitness, expressed in milieu exposing the possible selective advantage at given time and location. Given that protein may serve more than function, fitness should be assessed several dimensions. In present study we have explored individual mutational steps leading triple-point-mutated human GST (glutathione transferase) A2-2 displaying enhanced activity with azathioprine. A total eight...