A5059259815- Pregnancy and preeclampsia studies
- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Adenosine and Purinergic Signaling
- Angiogenesis and VEGF in Cancer
- Reproductive System and Pregnancy
- Birth, Development, and Health
- Neurological Complications and Syndromes
- Neonatal and fetal brain pathology
- Cardiovascular Issues in Pregnancy
- Gestational Diabetes Research and Management
- Mesenchymal stem cell research
- Kruppel-like factors research
- Inflammatory Biomarkers in Disease Prognosis
- Reservoir Engineering and Simulation Methods
- COVID-19 Impact on Reproduction
- Maternal Mental Health During Pregnancy and Postpartum
- Ectopic Pregnancy Diagnosis and Management
- Barrier Structure and Function Studies
- Hydrology and Watershed Management Studies
- Inflammatory mediators and NSAID effects
- Travel-related health issues
- Minimally Invasive Surgical Techniques
- Hepatitis Viruses Studies and Epidemiology
- Pharmacological Receptor Mechanisms and Effects
University of Bío-Bío
2014-2025
Universidad de Los Andes, Chile
2025
Instituto de Neurociencia Biomédica
2025
Johns Hopkins University
2023
Finis Terrae University
2020
University of Chile
2020
University of Concepción
2020
Complejo Asistencial Sótero del Río
1994
The inflammatory responses from synovial fibroblasts and macrophages the mitochondrial dysfunction in chondrocytes lead to oxidative stress, disrupt extracellular matrix (ECM) homeostasis, accelerate deterioration process of articular cartilage osteoarthritis (OA). In recent years, it has been proposed that mesenchymal stromal cells (MSC) transfer their functional mitochondria damaged response cellular becoming one mechanisms underpinning therapeutic effects. Therefore, we hypothesize a...
Cerebrovascular complications, including cerebral edema and ischemic hemorrhagic stroke, constitute the leading cause of maternal mortality associated with preeclampsia. The underlying mechanisms these cerebrovascular complications remain unclear. However, they are linked to placental dysfunction blood-brain barrier (BBB) disruption. Nevertheless, connection between two distant organs is still being determined. Increasing evidence suggests that placenta releases signaling molecules,...
Placentas from gestational diabetes mellitus (GDM) are often hypervascularized; however, participation of vascular endothelial growth factor (VEGF) and its receptors in this placental adaptation is unclear. We aimed to test whether changes phosphorylation tyrosine 951 or 1175 (pY951 pY1175) the receptor 2 (KDR) associated with proangiogenic state observed placentas GDM. obtained samples women normal pregnancies (n = 24) GDM 18). measured relative expression markers for cell number (CD31,...
Preeclampsia, a pregnancy-related endothelial disorder, is associated with both cardiovascular and cerebrovascular complications. Preeclampsia requires the presence of placenta as part its pathophysiology, yet role this organ in complications remains unclear. Research has shown that circulating small extracellular vesicles (also known exosomes) present preeclampsia plasma can generate dysfunction, but it unclear whether impairment function brain cells at blood-brain barrier secondary to...
Abstract Ischaemic stroke is a leading cause of death and disability. Circulating extracellular vesicles (EVs) post‐stroke may help brain endothelial cells (BECs) counter ischaemic injury. However data on how EVs from patients, considering injury severity, affect these are limited. The aims were to characterize the inflammatory angiogenic components circulating in acute investigate whether differentially influence proangiogenic properties blood–brain barrier (BBB) integrity human BECs....
Background: Eclampsia is a severe complication of preeclampsia involving blood-brain barrier (BBB) disruption. While small extracellular vesicles (sEVs) contribute to endothelial dysfunction in preeclampsia, their role eclampsia remains unclear. Magnesium sulfate (MgSO4), the standard treatment, may mitigate BBB injury. We examined effects plasma and plasma-derived sEVs from women with on integrity potential modulatory MgSO4. Methods: Plasma plasma-sEVs were isolated normotensive pregnancies...
Cerebral complications in preeclampsia contribute substantially to maternal mortality and morbidity. There is a lack of reliable accessible predictors for preeclampsia-related cerebral complications. In this study, plasma from women with (n = 28), normal pregnancies 28) non-pregnant 16) was analyzed concentrations the biomarkers neurofilament light (NfL), tau, neuron-specific enolase (NSE) S100B. Then, an vitro blood–brain barrier (BBB) model, based on human microvascular endothelial cell...
BACKGROUND: Children from pregnancies affected by preeclampsia have an increased risk of cognitive and behavioral alterations via unknown pathophysiology. We tested the hypothesis that generated reduced brain cortex angiogenesis in offspring. METHODS: The preeclampsia-like syndrome (PELS) mouse model was administering nitric oxide inhibitor NG-nitroarginine methyl ester hydrochloride. Confirmatory experiments were done using 2 additional PELS models. While vitro analysis used mice human...
We aimed to compare the cardiorespiratory, metabolic, and perceptual responses high- moderate-intensity eccentric cycling versus concentric in chronic obstructive pulmonary disease (COPD) patients. Ten patients with moderate COPD (forced expiratory volume 1 s (FEV ) = 68.6% ± 20.4% of predicted; 68.3 9.1 years) performed 30 min (CONC-M: 50% maximum workload; W max ), (ECC-M: high-intensity (ECC-H: 100% cycling. Average power output, oxygen consumption (V̇O 2 minute ventilation (VE),...
To investigate the functionality of<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mrow><mml:msub><mml:mrow><mml:mtext>A</mml:mtext></mml:mrow><mml:mrow><mml:mn>2</mml:mn><mml:mtext>B</mml:mtext></mml:mrow></mml:msub></mml:mrow></mml:math>adenosine receptor (<mml:math id="M2"><mml:mrow><mml:msub><mml:mrow><mml:mtext>A</mml:mtext></mml:mrow><mml:mrow><mml:mn>2</mml:mn><mml:mtext>B</mml:mtext></mml:mrow></mml:msub></mml:mrow></mml:math>AR) and nitric oxide (NO) vascular...
Estrogenic steroids and adenosine A2A receptors promote the wound healing angiogenesis processes. However, so far, it is unclear whether estrogen may regulate expression pro-angiogenic activity of receptors. Using in vivo analyses, we showed that female wild type (WT) mice have a more rapid process than or male A2A-deficient (A2AKO) mice. We also found pulmonary endothelial cells (mPEC) isolated from WT higher receptor mPEC were sensitive to A2A-mediated response, suggesting an ER crosstalk,...
BACKGROUND: The physiopathology of life-threatening cerebrovascular complications in preeclampsia is unknown. We investigated whether disruption the blood-brain barrier, generated using circulating small extracellular vesicles (sEVs) from women with or placentae cultured under hypoxic conditions, impairs expression tight junction proteins, such as CLDN5 (claudin-5), mediated by VEGF (vascular endothelial growth factor), and activation KDR (VEGFR2 [VEGF receptor 2]). METHODS: perform a...
The efficacy of an Oral Whole Cell ETEC Vaccine (OEV) against Travelers' Diarrhea (TD) was reexamined using novel outcome and immunologic measures. More specifically, a recently developed disease severity score alternative clinical endpoints were evaluated as part initial validation effort to access the vaccine intervention for first time in travelers endemic area. A randomized, double-blind, placebo-controlled trial followed Guatemala or Mexico up 28 days after arrival country following...
Abstract Background Physiopathology of life-treating cerebrovascular complications in preeclampsia are yet unknown. We investigated whether disruption the blood-brain barrier (BBB), generated using circulating small extracellular vesicles (sEVs) from women with or placentae cultured under hypoxic conditions, impairs expression tight junction proteins, such as claudin 5 (CLDN5), mediated by VEGF and activation receptor 2 (KDR). Methods sEVs were isolated plasma (normal pregnancy, sEVs-NP,...
ARTÍCULO DE REVISIÓN ResumenLa crisis sanitaria por la pandemia de COVID-19, enfermedad generada infección con el SARS-CoV-2, ha llevado a pérdida más 25,000 vidas en Chile, 370,000 mujeres entre 15 y 44 años confirmada.Se reportado una mayor vulnerabilidad las gestantes cuanto desarrollar cuadros COVID-19 graves o críticos, un aumento incidencia resultados obstétricos perinatales adversos.Es relevante considerar que alto porcentaje infectadas SARS-CoV-2 son asintomáticas, lo cual nos pone...