A5103074417- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Microfluidic and Bio-sensing Technologies
- Cancer Cells and Metastasis
- Cancer-related gene regulation
- Mass Spectrometry Techniques and Applications
- RNA and protein synthesis mechanisms
- Advanced biosensing and bioanalysis techniques
- Monoclonal and Polyclonal Antibodies Research
- Advanced Biosensing Techniques and Applications
- Orbital Angular Momentum in Optics
- Cancer Genomics and Diagnostics
- Cancer-related molecular mechanisms research
- Single-cell and spatial transcriptomics
- 3D Printing in Biomedical Research
- Lymphoma Diagnosis and Treatment
- Metabolomics and Mass Spectrometry Studies
- HER2/EGFR in Cancer Research
- Collagen: Extraction and Characterization
- Analytical Chemistry and Chromatography
- Molecular Communication and Nanonetworks
- Biosensors and Analytical Detection
- Innovative Microfluidic and Catalytic Techniques Innovation
- Carbon and Quantum Dots Applications
- Plant pathogens and resistance mechanisms
First Affiliated Hospital of Jiangxi Medical College
2022-2024
Jiangxi Provincial People's Hospital
2021-2024
Hubei Cancer Hospital
2011-2021
Nanchang University
2021
Huazhong University of Science and Technology
2018-2021
Wuhan University
2015-2020
State Council of the People's Republic of China
2020
Wuhan Institute of Bioengineering
2016-2018
Jiangsu Academy of Agricultural Sciences
2009
DNA methylation (5-methylcytosine, 5-mC) is the best characterized epigenetic mark that has regulatory roles in diverse biological processes. Recent investigation of RNA modifications also raises possible functions adenine and cytosine methylations on gene regulation form "RNA epigenetics." Previous studies demonstrated global hypomethylation tumor tissues compared to healthy controls. However, circulating cells (CTCs) are derived from tumors still a mystery due lack proper analytical...
Cytosine methylation (5-methylcytosine, 5-mC) in genomic DNA is an important epigenetic mark that has regulatory roles diverse biological processes. 5-mC can be oxidized stepwise by the ten–eleven translocation (TET) proteins to form 5-hydroxymethylcytosine (5-hmC), 5-formylcytosine (5-foC), and 5-carboxylcytosine (5-caC), which constitutes active demethylation pathway mammals. Owing extremely limited contents of endogenous oxidation products, no reported method directly determine all these...
Chemical labeling coupled with LC-MS enables the sensitive and simultaneous detection of oxidative products 5-methylcytosine. With this method, we can determine 5-methylcytosine, 5-hydroxymethylcytosine, 5-formylcytosine 5-carboxylcytosine in RNA mammals.
Some modified ribonucleosides in biological fluids have been evaluated as cancer-related metabolites. Detection of endogenous may serve a noninvasive cancers diagnostic method. However, determination is still challenging because their low abundance and serious matrix interferences fluids. Here, we developed novel strategy for comprehensive profiling ribose conjugates from using metal oxide-based dispersive solid-phase extraction (DSPE) followed with vitro stable isotope labeling double...
Abstract RNA molecules harbor diverse modifications that play important regulatory roles in a variety of biological processes. Over 150 have been identified molecules. N6-methyladenosine (m6A) and 1-methyladenosine (m1A) are prevalent occurring various species mammals. Apart from the single methylation adenosine (m6A m1A), dual modification nucleobase adenosine, such as N6,N6-dimethyladenosine (m6,6A), also has reported to be present Whether there other forms than m6,6A remains elusive....
RNA contains a large number of modified nucleosides. In the metabolic re-exchange RNA, nucleosides cannot be recycled and are thus excreted from cells into biological fluids. Determination endogenous in fluids may serve as non-invasive cancers diagnostic methods. Here we prepared boronate-affinity organic-silica hybrid capillary monolithic column (BOHCMC) that exhibited excellent selectivity toward cis-diol-containing compounds. We then used BOHCMC on-line solid-phase microextraction (SPME)...
Profiling the heterogeneous phenotypes of individual circulating tumor cells (CTCs) from patients is a very challenging task, but it paves new ways for cancer management, especially personalized anticancer therapy. Herein, we propose chip-assisted multifunctional-nanosphere system efficient and reliable biomarker phenotype analysis CTCs. Red fluorescent magnetic biotargeting multifunctional nanospheres green targeting to two kinds CTC biomarkers are used convenient dual-fluorescence labeling...
Abstract Comprehensive phenotypic profiling of heterogeneous circulating tumor cells (CTCs) at single‐cell resolution has great importance for cancer management. Herein, a novel spectrally combined encoding (SCE) strategy was proposed multiplex biomarker single CTCs using multifunctional nanosphere‐mediated microfluidic platform. Different cellular biomarkers uniquely labeled by nanosphere barcodes, possessing identical magnetic tags and distinct optical signatures, enabled isolation with...
The uncontrollable migration of cancer cells is one the reasons for poor prognosis. interaction between collagen and cellular processes usually dominated by full triple‐helix structure collagen. However, it remains to be explored how configuration affects behaviors cells. Michigan foundation‐7 are chosen study this relationship. Meanwhile, grass carp representative fish species in Hubei virtually unaffected zoonotic diseases. Therefore, carp‐derived prepared used modulate behaviors. sample...
Stem cells serve as promising candidates for regenerative medicine. The migration capability of stem may determine the treatment efficacy. Currently, chemotaxis and thigmotaxis are two plausible approaches to regulate...
5-Methylcytosine (5-mC) is an important epigenetic mark that plays critical roles in a variety of cellular processes. To properly exert physiological functions, the distribution 5-mC needs to be tightly controlled both DNA and RNA. In addition methyltransferase-mediated RNA methylation, premethylated nucleotides can potentially incorporated into during replication transcription. exclude premodified RNA, endogenous 5-methyl-2'-deoxycytidine monophosphate (5-Me-dCMP) generated from nucleic...
As an emerging fascinating fluorescent nanomaterial, carbon nanodots (CDs) have attracted much attention owing of their unique properties such as small size, antiphotobleaching, and biocompatibility. However, its use in biomedical analysis is limited because low quantum yield. Herein, we constructed a dual amplification fluorescence sensor by incorporating immunohybridization chain reaction (immuno-HCR) metal-enhanced (MEF) CDs for the detection alpha fetal protein (AFP). The immunoplasmonic...
RNA molecules harbor diverse chemical modifications that play important regulatory roles in a variety of biological processes. Up to date, more than 150 have been identified various species. Most these occurring nucleic acids are the methylation acids. It has demonstrated many reversible and undergo dynamic demethylation. Previous studies established demethylation two most prevalent 5-methylcytidine (m5C) N6-methyladenosine (m6A) is through hydroxylation m5C m6A, forming...
Establishment of a stable analytical methodology with high-quality results is an urgent need for screening cancer biomarkers in early diagnosis cancer. In this study, we incorporate holographic optical tweezers upconversion luminescence encoding to design imageable suspension array and apply it conduct the detection two liver related biomarkers, carcinoembryonic antigen alpha fetal protein. This bead-based assay actualized by forming bead synchronously exciting corresponding trapped complex...
The discovery of dynamic and reversible modifications in RNA expands their functional repertoires. Now, have been viewed as new regulators involved a variety biological processes. Among these modifications, thiolation is one kind special modification RNA. Several thiouridines identified to be present RNA, they are essential the natural growth metabolism cells. However, detection generally challenging, few studies could offer quantitative levels uridine which limits in-depth elucidation...
Background: The epidermal growth factor receptor (EGFR) is a promising therapeutic target in cancer, but its clinical value breast cancer remains controversial. Our previous studies have found that quantitative analysis of biomarkers with quantum dot-based nanotechnology had better detection performance than conventional immunohistochemistry. present study was undertaken to investigate the prognostic EGFR using spectral analysis. Methods: expression 65 specimens detected by...
Ribonucleotide analogues and their related phosphorylated metabolites play critical roles in tumor metabolism. However, determination of the endogenous ribonucleotides from complex biological matrix is still a challenge due to high structural similarity polarity that will lead low retention detection sensitivities by liquid chromatogram mass spectrometry analysis. In this study, we developed diazo reagent labeling strategy with analysis for sensitive living organism. A pair light heavy...
Anaplastic diffuse large B-cell lymphoma (A-DLBCL) is a rare morphologic variant characterized by the presence of polygonal, bizarre-shaped tumor cells. However, clinicopathologic and genetic features this are largely unknown. In study, we investigated 35 cases A-DLBCL with regard to their clinical, pathologic, characteristics. The age patients ranged from 23 89 years (median age, 62 y) male female ratio 23:12. Twenty-two 26 (85%) had Ann Arbor stage III or IV disease, 17/26 (65%)...