A5100629490- Collagen: Extraction and Characterization
- CRISPR and Genetic Engineering
- Protein Hydrolysis and Bioactive Peptides
- MicroRNA in disease regulation
- Bacteriophages and microbial interactions
- Circular RNAs in diseases
- biodegradable polymer synthesis and properties
- Cancer-related molecular mechanisms research
- Bone Tissue Engineering Materials
- Silk-based biomaterials and applications
- RNA Interference and Gene Delivery
- RNA and protein synthesis mechanisms
- Glaucoma and retinal disorders
- Advanced biosensing and bioanalysis techniques
- Molecular Biology Techniques and Applications
- Electrospun Nanofibers in Biomedical Applications
- DNA and Nucleic Acid Chemistry
- Extracellular vesicles in disease
- Renal and related cancers
- Cellular Mechanics and Interactions
- Reproductive System and Pregnancy
- Retinal Diseases and Treatments
- Retinal and Optic Conditions
- Microbial Community Ecology and Physiology
- Nanocluster Synthesis and Applications
Guangdong Academy of Forestry
2025
Wuhan Polytechnic University
2016-2024
Henan Agricultural University
2020-2024
Ningbo University Affiliated Hospital
2024
Southern University of Science and Technology
2022-2024
Ningbo University
2021-2024
Affiliated Eye Hospital of Wenzhou Medical College
2024
Wenzhou Medical University
2021-2024
University of Nottingham Ningbo China
2024
First Teaching Hospital of Tianjin University of Traditional Chinese Medicine
2019-2024
Abstract Exosomes are nanosized membrane vesicles (30–100 nm) that can easily penetrate the blood–brain barrier, safely deliver therapeutic drugs, and be modified with target ligands. Embryonic stem cells (ESCs) provide abundant exosome sources for clinical application due to their almost unlimited self‐renewal. Previous studies show exosomes secreted by ESCs (ESC‐exos) have antitumor properties. However, it is not known whether ESC‐exos inhibit glioblastoma (GBM) growth. In this study,...
The myophage possesses a contractile tail that penetrates its host cell envelope. Except for investigations on the bacteriophage T4 with rather complicated structure, assembly pattern and contraction mechanism of remain largely unknown. Here, we present fine structure freshwater Myoviridae cyanophage Pam3, which has an icosahedral capsid ~680 Å in diameter, connected via three-section neck to 840-Å-long tail, ending three-module baseplate composed only six protein components. This simplified...
Abstract Silent information regulator 2 (Sir2) proteins typically catalyze NAD + -dependent protein deacetylation. The recently identified bacterial Sir2 domain-containing protein, defense-associated sirtuin (DSR2), recognizes the phage tail tube and depletes to abort propagation, which is counteracted by phage-encoded DSR anti-defense 1 (DSAD1), but their molecular mechanisms remain unclear. Here, we determine cryo-EM structures of inactive DSR2 in its apo form, DSR2–DSAD1 DSR2–DSAD1–NAD ,...
Abstract Background Postmenopausal osteoporosis (PMO) that results from estrogen withdrawal is the most common primary among older women. However, little known about mechanism of PMO, and effective treatment PMO limited. Methods We used real-time polymerase chain reaction (qPCR), Western blotting, RNA pull down to investigate relationship between miR-186 MOB Kinase Activator 1A (Mob1). Also, we investigated effect exosome in osteogenesis using alkaline phosphatase (ALP) staining. And...
Rationale: MicroRNAs (miRNAs) are key regulators of vascular development and diseases. The function underlying mechanism endothelial miRNAs have not been fully defined. Objective: To investigate the role miR-126 in zebrafish development. Methods Results: Two homologs miR-126, miR-126a (namely previous literature) miR-126b, with only 1 nucleotide difference their mature sequences, were identified genome. In vitro analysis showed that both precursors could sufficiently produce functional...
Green and effective synthesis of flavor esters is a long-term interest in food, cosmetic pharmaceutical industries. Here, an immobilized lipase microarray was prepared by immobilizing CSL on hydrophobically modified ordered mesoporous silicon ([email protected]8) for the enzymatic production cinnamic with different flavors. The results showed that [email protected]8, immobilization capacity 249.3 ± 11.1 mg/g, exhibited 1.6 times higher catalytic activity than free at optimum temperature 70...
Abstract Probiotics have been characterized as useful for maintaining the balance of host gut flora and conferring health effects, but few studies focused on their potential delaying aging in host. Here we show that Lacticaseibacillus rhamnosus Probio-M9 (Probio-M9), a healthy breast milk probiotic, enhances locomotor ability slows decline muscle function model organism Caenorhabditis elegans . Live significantly extends lifespan C. dietary restriction-independent manner. By screening...
Differences between CDs prepared by microwave reaction and conventional hydrothermal methods were systematically compared to guide the optimization of parameters biomass-derived CDs.
The uncontrollable migration of cancer cells is one the reasons for poor prognosis. interaction between collagen and cellular processes usually dominated by full triple‐helix structure collagen. However, it remains to be explored how configuration affects behaviors cells. Michigan foundation‐7 are chosen study this relationship. Meanwhile, grass carp representative fish species in Hubei virtually unaffected zoonotic diseases. Therefore, carp‐derived prepared used modulate behaviors. sample...
Stem cells serve as promising candidates for regenerative medicine. The migration capability of stem may determine the treatment efficacy. Currently, chemotaxis and thigmotaxis are two plausible approaches to regulate...
Chirality is one of the fundamental biochemical properties in a living system, and lot biological physiological processes are greatly influenced by chirality molecules. Inspired this phenomenon, we study covalent assembly DNA on chiral molecule modified surfaces further discuss hybridization with nucleic acids. Take methylene blue (MB) as model molecule, show that peak current L-NIBC (NIBC, N-isobutyryl-L(D)-cysteine) gold surface (L-surface) larger than D-surface because stronger...
The enzyme immobilization based on metal affinity has been applied in bioengineering and biochemical fields, but the effects of different ions ligand remain unexplored systematically. Here, were initially chelated with triamino ligands Alcalase-immobilized hollow mesoporous silica spheres (HMSS) through metal–protein interaction forces. results indicate coordinated enzymes increased increase ions, from which transition (Zn2+, Cu2+ Ni2+) easier to chelate than hard lewis acids (Fe3+, Ca2+)....
Xenogeneic collagen coassembly (XCCA) offers a new view for the design and performance regulation of novel collagen-based biomaterials. But there is still lack accurate sensitive method monitoring XCCA. In this study, simple efficient graphene-oxide (GO)-based fluorescence resonance energy transfer (FRET) platform has been developed to sense We first designed fluorescein isothiocyanate (FITC)-labeled porcine skin (PSC) that adsorbed on GO surface effectively quenched its fluorescence. Upon...
Abstract The RNA-targeting type III-E CRISPR-gRAMP effector interacts with a caspase-like protease TPR-CHAT to form the CRISPR-guided caspase complex (Craspase), but their functional mechanism is unknown. Here, we report cryo-EM structures of gRAMP crRNA and -TPR-CHAT complexes, before after either self or non-self RNA target binding, elucidate mechanisms underlying RNA-induced activation. associated adopted distinct conformation upon versus nucleotides at positions −1 −2 CRISPR-derived...