A5101157524- Renal cell carcinoma treatment
- Renal and related cancers
- Ferroptosis and cancer prognosis
- Respiratory Support and Mechanisms
- Pancreatitis Pathology and Treatment
- Intensive Care Unit Cognitive Disorders
- Ovarian cancer diagnosis and treatment
- Petroleum Processing and Analysis
- COVID-19 Clinical Research Studies
- Hydrocarbon exploration and reservoir analysis
- Clinical Nutrition and Gastroenterology
- Abdominal Surgery and Complications
- Electrolyte and hormonal disorders
- Crystallization and Solubility Studies
- Sepsis Diagnosis and Treatment
- X-ray Diffraction in Crystallography
- Radiation Therapy and Dosimetry
- Long-Term Effects of COVID-19
- Child Nutrition and Feeding Issues
- Immune Response and Inflammation
- DNA Repair Mechanisms
- Lipoproteins and Cardiovascular Health
- Anesthesia and Sedative Agents
- Ultrasound in Clinical Applications
- Circular RNAs in diseases
West China Hospital of Sichuan University
2015-2025
Sichuan University
2015-2025
Chongqing University
2024
Central Hospital of Zibo
2018-2024
Zhejiang Chinese Medical University
2024
Nanjing Medical University
2022
Beijing University of Technology
2022
Southwest Jiaotong University
2022
First People's Hospital of Nanning
2022
Chinese Academy of Sciences
2007-2021
Experimental animal models of acute respiratory distress syndrome (ARDS) have shown that the updated airway pressure release ventilation (APRV) methodologies may significantly improve oxygenation, maximize lung recruitment, and attenuate injury, without circulatory depression. This led us to hypothesize early application APRV in patients with ARDS would allow pulmonary function recover faster reduce duration mechanical as compared low tidal volume protective (LTV).A total 138 who received...
To clarify the roles of organ failure and infection in outcome necrotizing pancreatitis.Results previous cohort studies that focused on acute pancreatitis are controversial.In this study, we collected medical records 447 patients with from January 2009 to June 2012. Data associated were analyzed.The overall mortality rate was 13% (58/447). Intervention performed 223 patients. Among these patients, 134 confirmed be infected necrosis by a positive culture. The 15% (13/89) sterile group 18%...
Objectives: This study aimed to evaluate the effectiveness of a pharmacist-led intervention in decreasing overuse stress ulcer prophylaxis (SUP) compared with usual care for adult patients Chinese ICUs. Design: Pragmatic, multicenter, stepped-wedge, cluster-randomized controlled trial. Setting: Twenty-six ICUs China from October 2022 March 2023. Patients: We enrolled 2199 18 years old or older who were newly admitted participating Interventions: Using Medical Research Council framework...
Declared as pandemic by WHO, the coronavirus disease 2019 (COVID-19) pneumonia has brought great damage to human health. The uncontrollable spread and poor progression of COVID-19 have attracted much attention from all over world. We designed this study develop a prognostic nomogram incorporating Prognostic nutritional index (PNI) in patients.Patients confirmed with treated Renmin Hospital Wuhan University January February 2020 were included study. used logistic regression analysis find risk...
The worldwide urbanization has resulted in a massive loss of natural habitats, which is threatening biodiversity and socio-ecological sustainability. Urban rewilding strategy aiming to restore the complexity, autonomy self-sustainability urban ecosystems by interlinking ecological processes while minimizing or gradually reducing human disturbances. benefits are widely acknowledged; however, there remains knowledge gap concerning identification suitable locations for methodologies assessing...
This study aimed to address whether hydroxyethyl starch (HES) is beneficial for intra-abdominal pressure (IAP) in severe acute pancreatitis (SAP) early stages.Forty-one patients with SAP were randomized HES group (n = 20) and the Ringer's lactate (RL) 21). The groups received 6% 130/0.4 8 days RL solution without colloid, respectively. primary end point was IAP. secondary points fluid balance, major organ complications, Acute Physiology Chronic Heath Evaluation II score, serum levels of...
Midazolam and propofol used alone for long-term sedation are associated with adverse effects. Sequential use may reduce the effects, lead to faster recovery, earlier extubation lower costs. This study evaluates safety, cost of midazolam, propofol, their sequential in critically ill mechanically ventilated patients.A total 135 patients who required mechanical ventilation >3 days were randomly assigned receive midazolam (group M), P), or both M-P). In group M-P, was switched until passed...
<div>Abstract<p>Fumarate hydratase (FH) deficiency causes hereditary leiomyomatosis and renal cell carcinoma (RCC). FH-deficient tumors lack effective therapeutic options. Here, we utilized an epigenetic-focused single-guide RNA library to elucidate potential drug targets in tumors. The screen identified chromodomain helicase DNA-binding protein 6 (CHD6) as essential regulator of the growth FH-mutated RCC. Mechanically, FH loss induced fumarate-mediated succinylation inactivation...
<p>Characteristics of patients whose tumors were used for PDOs or PDXs experiments, Related to Figure 4&7.</p>
<p>Supplementary Figure 2. FH deficiency accumulates CHD6 via inactivating Keap1, related to 2&3. A. WB and co-IP analysis indicating no interactions between VHL proteins in WCLs of ACHN cells. B. (left) RT-qPCR (right) indicated the protein or mRNA levels control VHL-depleted RCC C. Schematic illustration Keap1 deletion mutants. The binding capacity is with symbol. D. Western blots showing vitro ubiquitination assays conducted by incubating reconstituted Keap1–CUL3–RBX1 E3...
<p>Summary of the epigenetic regulators showing decreased sgRNA abundance in UOK-262 and ACHN cells, respectively.</p>
<p>Supplementary Figure 5. CHD6/SMARCA2/4-coordinated SEs-promoter looping boosts the transcription of NF-κB-related targets, related to 6. A. 3C assay indicated genes in WT, CHD6-KO Caki-2 cells with or without DMF stimulation. “Pro” means input-pro, while “3C” primers targeting enhancer region. B. ChIP-qPCR H3K27ac markers promoters enhancers CCL2, ICAM1, BCL3 gene UOK-262 (n = 5). C. Immunoblotting analysis p65 immunoprecipitates CHD6 depletion. D. SMARCA2/4 SEs 5) restoration. E....
<p>Identification of super-enhancer-associated genes in DMF-treated ACHN cells, related to Figure 6.</p>
<p>Oligonucleotides, related to Methods.</p>
<p>Supplementary Figure 1. Library and cells used for in vivo epigenetic CRISPR screen, related to A. Schematic diagram of FH mutations distributions the indicated protein domains. B. Intracellular fumarate levels were measured a panel RCC cell lines. C. Lorenz curve showing distribution sgRNAs epigenetic-focused library. D. Workflow generation UOK- or ACHN-clones without Cas9 evaluating guides that persist upon tumour formation from initiating (TICs), with further screens. E. Western...
<p>Supplementary Figure 4. CHD6 activates NF-κB signaling to potentiate FH-deficient RCC malignancy, related 5. A. Volcano plot showing differentially expressed genes in UOK-262 cells upon knockdown. B. Unsupervised cluster analysis of control and knockdown cells. C. ATAC-seq signals the profiles OCRs across indicated peaks UOK262 with versus without D. Heatmap exhibiting significance transcription factor motifs enriched accessible loci derived from CHD6-KD motif is dominantly...
<p>Supplementary Figure 6. In vitro and in vivo assessment of AU-15330 efficacy against FH-deficient RCC multiple preclinical models, related to 7. A. Dose-response curves IC50 FH-intact or cells treated with AU-15330. B. UOK-262 AU-15330, AU-15139, AU-16235. C-D. Mouse weight changes measurements (C) (n = 5) complete blood counts (D) performed on vehicle control mice as Fig. 7A. E. Representative HE graphs showing the morphology critical organs an increased amount F. FH-mutated...
<p>Supplementary Figure 3. CHD6 is essential for FH-deficient RCC cells, related to 4. A-B. MTT analysis of FH-WT PRCC (A) or canonical ccRCC (B) cells with without CHD6-KD. C. Competition-based assay measure the effect CHD4 shRNA on growth UOK-262 (n = 3 per time point). CHD4-KD were identified by coexpression green fluorescent protein (GFP) (LMN vector). The percentage GFP+ was thus tracked over 12 days and normalized GFP day 2. D. Quantified BIL signals orthotopic (FH-intact Caki-2)...
<p>Supplementary Figure 1. Library and cells used for in vivo epigenetic CRISPR screen, related to A. Schematic diagram of FH mutations distributions the indicated protein domains. B. Intracellular fumarate levels were measured a panel RCC cell lines. C. Lorenz curve showing distribution sgRNAs epigenetic-focused library. D. Workflow generation UOK- or ACHN-clones without Cas9 evaluating guides that persist upon tumour formation from initiating (TICs), with further screens. E. Western...
<p>Supplementary Figure 4. CHD6 activates NF-κB signaling to potentiate FH-deficient RCC malignancy, related 5. A. Volcano plot showing differentially expressed genes in UOK-262 cells upon knockdown. B. Unsupervised cluster analysis of control and knockdown cells. C. ATAC-seq signals the profiles OCRs across indicated peaks UOK262 with versus without D. Heatmap exhibiting significance transcription factor motifs enriched accessible loci derived from CHD6-KD motif is dominantly...
<p>Supplementary Figure 6. In vitro and in vivo assessment of AU-15330 efficacy against FH-deficient RCC multiple preclinical models, related to 7. A. Dose-response curves IC50 FH-intact or cells treated with AU-15330. B. UOK-262 AU-15330, AU-15139, AU-16235. C-D. Mouse weight changes measurements (C) (n = 5) complete blood counts (D) performed on vehicle control mice as Fig. 7A. E. Representative HE graphs showing the morphology critical organs an increased amount F. FH-mutated...
<p>Characteristics of patients whose tumors were used for PDOs or PDXs experiments, Related to Figure 4&7.</p>
<p>Supplementary Figure 5. CHD6/SMARCA2/4-coordinated SEs-promoter looping boosts the transcription of NF-κB-related targets, related to 6. A. 3C assay indicated genes in WT, CHD6-KO Caki-2 cells with or without DMF stimulation. “Pro” means input-pro, while “3C” primers targeting enhancer region. B. ChIP-qPCR H3K27ac markers promoters enhancers CCL2, ICAM1, BCL3 gene UOK-262 (n = 5). C. Immunoblotting analysis p65 immunoprecipitates CHD6 depletion. D. SMARCA2/4 SEs 5) restoration. E....