A5034915652- Renal cell carcinoma treatment
- Ferroptosis and cancer prognosis
- Renal and related cancers
- Extracellular vesicles in disease
- Spinal Cord Injury Research
- Epigenetics and DNA Methylation
- Ovarian cancer diagnosis and treatment
- Prostate Cancer Treatment and Research
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Neuroinflammation and Neurodegeneration Mechanisms
- Autophagy in Disease and Therapy
- Cancer Immunotherapy and Biomarkers
- Bladder and Urothelial Cancer Treatments
- Cancer-related gene regulation
- Prostate Cancer Diagnosis and Treatment
- Spinal Dysraphism and Malformations
- Nerve injury and regeneration
- Protein Degradation and Inhibitors
- Sirtuins and Resveratrol in Medicine
- Cancer Genomics and Diagnostics
- RNA and protein synthesis mechanisms
- Peptidase Inhibition and Analysis
- Hydrogels: synthesis, properties, applications
- Viral Infections and Immunology Research
University of Science and Technology of China
2024-2025
Wuhan Children's Hospital
2020-2024
Huazhong University of Science and Technology
2020-2024
Jinzhou Medical University
2020-2024
Ruijin Hospital
2019-2024
Shanghai Jiao Tong University
2019-2024
Wuhan University
2008-2024
Jingzhou Maternal and Child Health Hospital
2024
Fudan University
2021-2024
China Astronaut Research and Training Center
2024
Whether tumor mutation burden (TMB) correlated with improved survival outcomes or promotion of immunotherapies remained controversy in various malignancies. We aimed to investigate the prognosis TMB and potential association immune infiltrates clear cell renal carcinoma (ccRCC).We downloaded somatic data 336 ccRCC patients from Cancer Genome Atlas (TCGA) database, analyzed profiles "maftools" package. was calculated we classified samples into high-TMB low-TMB group. Differential analysis...
Background: Methyltransferase-like 14 (METTL14) participates in tumorigenesis several malignancies, but how METTL14 mediates the metastasis of renal cell carcinoma (RCC) has never been reported. Methods: Western blotting, quantitative real-time PCR, and immunohistochemistry were used to determine mRNA protein levels relevant genes. Methylated RNA immunoprecipitation sequencing utilized screen potential targets METTL14. Chromatin assay for transposase-accessible chromatin performed...
Only a minority of rheumatoid arthritis (RA) patients achieve disease remission, so the exploration additional pathogenic factors and development new therapeutics are needed. Here, strong correlations among cell-free DNA (cfDNA) level inflammatory response in clinical synovial fluid samples RA activity discovered. The important role cfDNA collagen-induced (CIA) murine model is also demonstrated. Building on these findings, novel therapeutic based anti-inflammatory (M2) macrophage-derived...
Chimeric antigen receptor (CAR)–T cell therapies have revolutionized the landscape of cancer treatment, in particular context hematologic malignancies. However, for solid tumors that lack tumor-specific antigens, CAR-T cells can infiltrate and attack nonmalignant tissues expressing CAR target antigen, leading to on-target, off-tumor toxicity. Severe toxicities been observed clinical trials therapy tumors, highlighting need address this issue. Here, we demonstrated targeting adhesion...
The papillary renal cell carcinoma (pRCC) is a rare subtype of with limited investigation. Our study aimed to explore robust signature predict the prognosis pRCC from perspective mutation profiles.In this study, we downloaded simple nucleotide variation data 288 samples Cancer Genome Atlas (TCGA) database. "GenVisR" package was utilized visualize gene profiles in pRCC. PPI network conducted based on STRING database and modification performed via Cytoscape software (Version 3.7.1). Top 50...
Hypoxia-inducible factor 2α (HIF2α) antagonists are effective against clear cell renal carcinomas (ccRCCs) that highly express HIF2α. To identify potential drug targets in HIF2α low/− ccRCC, we constructed an epigenetic-focused single-guide RNA library and performed vivo CRISPR-Cas9 knockout screen BALB/c nude mice transplanted with 786-O (HIF2α high ) or Caki-2 cells. We found the m6A demethylase fat mass obesity-associated ( FTO gene was indispensable to growth of but not ccRCC. Activation...
Uncontrollable inflammation and nerve cell apoptosis are the most destructive pathological response after spinal cord injury (SCI). So, suppression combined with neuroprotection is one of promising strategies to treat SCI. Engineered extracellular vesicles anti-inflammatory neuroprotective properties candidates for implementing these treatment SCI.By combining growth factor (NGF) curcumin (Cur), we prepared stable engineered approximately 120 nm from primary M2 macrophages (Cur@EVs-cl-NGF)....
This study aims to investigate the potential therapeutic effect of exosomes derived from macrophages loaded with curcumin (Exos-cur) on healing diabetic wounds. As a new type biomaterial, Exos-cur has better stability, anti-inflammation, and antioxidation biological activity. In in vitro experiments, can promote proliferation, migration, angiogenesis HUVECs (human umbilical vein endothelial cells) while reducing ROS (reactive oxygen species) produced by induced high glucose, regulating...
KIAA0101 overexpression was detected in numerous malignant solid tumors and involved tumor progression; however, the correlation between expression level human hepatocellular carcinoma (HCC) controversial. Our data revealed abnormal of transcript variant 1 (KIAA0101 tv1) at both messenger RNA protein levels HCC tissues cell lines assessed by semiquantitative reverse-transcription polymerase chain reaction (RT-PCR), virtual northern blot, western immunohistochemical analysis, especially stage...
Prostate cancer stemness (PCS) cells have been reported to drive tumor progression, recurrence and drug resistance. However, there is lacking systematical assessment of stemlike indices associations with immunological properties in prostate adenocarcinoma (PRAD). We thus collected 7 PRAD cohorts 1465 men calculated the for each sample using one-class logistic regression machine learning algorithm. selected mRNAsi quantify that correlated significantly prognosis accordingly identified 21...
Abstract Ferroptosis inducers have shown therapeutic potential in prostate cancer (PCa), but tumor heterogeneity poses a barrier to their efficacy. Distinguishing the regulators orchestrating metabolic crosstalk between cells could shed light on strategies more robustly activate ferroptosis. Here, we found that aberrant accumulation of jumonji domain containing 6 (JMJD6) proteins correlated with poorer prognosis PCa patients. Mechanistically, PCa-associated speckle type BTB/POZ protein...
<div>Abstract<p>Fumarate hydratase (FH) deficiency causes hereditary leiomyomatosis and renal cell carcinoma (RCC). FH-deficient tumors lack effective therapeutic options. Here, we utilized an epigenetic-focused single-guide RNA library to elucidate potential drug targets in tumors. The screen identified chromodomain helicase DNA-binding protein 6 (CHD6) as essential regulator of the growth FH-mutated RCC. Mechanically, FH loss induced fumarate-mediated succinylation inactivation...
<p>Characteristics of patients whose tumors were used for PDOs or PDXs experiments, Related to Figure 4&7.</p>
<p>Supplementary Figure 2. FH deficiency accumulates CHD6 via inactivating Keap1, related to 2&3. A. WB and co-IP analysis indicating no interactions between VHL proteins in WCLs of ACHN cells. B. (left) RT-qPCR (right) indicated the protein or mRNA levels control VHL-depleted RCC C. Schematic illustration Keap1 deletion mutants. The binding capacity is with symbol. D. Western blots showing vitro ubiquitination assays conducted by incubating reconstituted Keap1–CUL3–RBX1 E3...
<p>Summary of the epigenetic regulators showing decreased sgRNA abundance in UOK-262 and ACHN cells, respectively.</p>
<p>Supplementary Figure 5. CHD6/SMARCA2/4-coordinated SEs-promoter looping boosts the transcription of NF-κB-related targets, related to 6. A. 3C assay indicated genes in WT, CHD6-KO Caki-2 cells with or without DMF stimulation. “Pro” means input-pro, while “3C” primers targeting enhancer region. B. ChIP-qPCR H3K27ac markers promoters enhancers CCL2, ICAM1, BCL3 gene UOK-262 (n = 5). C. Immunoblotting analysis p65 immunoprecipitates CHD6 depletion. D. SMARCA2/4 SEs 5) restoration. E....
<p>Identification of super-enhancer-associated genes in DMF-treated ACHN cells, related to Figure 6.</p>
<p>Oligonucleotides, related to Methods.</p>
<p>Supplementary Figure 1. Library and cells used for in vivo epigenetic CRISPR screen, related to A. Schematic diagram of FH mutations distributions the indicated protein domains. B. Intracellular fumarate levels were measured a panel RCC cell lines. C. Lorenz curve showing distribution sgRNAs epigenetic-focused library. D. Workflow generation UOK- or ACHN-clones without Cas9 evaluating guides that persist upon tumour formation from initiating (TICs), with further screens. E. Western...