A5008386981- Lymphatic System and Diseases
- Cancer Cells and Metastasis
- Infectious Diseases and Mycology
- Fungal Infections and Studies
- Veterinary Oncology Research
- Skin and Cellular Biology Research
- Angiogenesis and VEGF in Cancer
- Immunotherapy and Immune Responses
- Wound Healing and Treatments
- Lung Cancer Diagnosis and Treatment
- Cell Adhesion Molecules Research
- Photodynamic Therapy Research Studies
- HER2/EGFR in Cancer Research
- Optical Coherence Tomography Applications
- Lung Cancer Treatments and Mutations
- Chemokine receptors and signaling
- Monoclonal and Polyclonal Antibodies Research
- Cancer, Stress, Anesthesia, and Immune Response
- Electromagnetic Fields and Biological Effects
- Lung Cancer Research Studies
- Prostate Cancer Treatment and Research
- Nanoplatforms for cancer theranostics
- Cancer Mechanisms and Therapy
- Cancer Immunotherapy and Biomarkers
- Cancer Research and Treatments
The University of Tokyo
2019-2025
Tokyo Medical University
2001-2012
Background Targeting regulatory T cell (Treg) infiltration is an emerging strategy for cancer immunotherapy. However, its efficacy in advanced prostate remains unclear. Here, we showed the therapeutic of anti-Treg treatment a canine model cancer. Methods We used dogs with naturally occurring to study molecular mechanism underlying Treg and effect treatment. Tumor-infiltrating Tregs was evaluated by immunohistochemistry, association prognosis examined spontaneous The explored RNA sequencing...
Human epidermal growth factor receptor 2 (HER2) is a known therapeutic target in canine osteosarcoma (OSA); however, the efficacy of anti-HER2 antibody drugs remains unclear. This study aimed to investigate effects including trastuzumab and emtansine (T-DM1) on OSA cell lines vitro vivo. Four (HMPOS, POS, OOS, HOS) were used. Western blotting revealed HER2 protein expression all lines. Although water-soluble tetrazolium salt assays showed inhibitory activity T-DM1 against vitro, was...
Canine urothelial carcinoma (cUC) is the most common tumor of lower urinary tract in dogs. Although chemotherapy and radical surgery have improved overall survival, dogs with cUC succumb to metastasis or recurrence. Therefore, development an effective systematic therapy warranted. In this study, a comprehensive drug screening test using cell line was performed anti-tumor effect histone deacetylase (HDAC) inhibitor evaluated. Comprehensive on cells. Based screening, anti-proliferation...
Although an association between prognosis and lobar location of lung cancer, particularly the left lower lobe (LLL), has been suggested, certainty such remains controversial. The purpose this study was to evaluate impact tumour on surgical outcomes as independent prognostic factor for survival in our non-small cell cancer (NSCLC) patient series. We retrospectively reviewed 978 NSCLC patients who underwent complete resection hospital 2000 2007. statistically analysed clinicopathological...
Podoplanin (PDPN), a small transmembrane mucin-like glycoprotein, is ectopically expressed on tumor cells. PDPN known to be linked with several aspects of malignancies in certain types human and canine tumors. Therefore, it considered novel therapeutic target. Monoclonal antibodies targeting cells showed obvious anti-tumor effects preclinical studies using mouse models. Previously, we generated cancer-specific mouse–dog chimeric anti-PDPN antibody, P38Bf, which specifically recognizes In...
Canine anal sac gland carcinoma (ASGC) frequently occurs in the apocrine glands of canine and shows aggressive biological behavior. The expression human epidermal growth factor receptor 2 (HER2) has been reported various tumors. HER2 is a promising therapeutic target these tumors, HER2-targeted drugs, such as trastuzumab lapatinib, have improved outcome patients. In this study, ASGC was evaluated to investigate its potential for ASGC. mRNA surgically resected tissues significantly higher...
Podoplanin (PDPN), a small transmembrane mucin-like glycoprotein, is ectopically expressed. It also known to be linked with several aspects of tumor malignancy in some types human tumors, including invasion, metastasis, and cancer stemness. However, there are few reports on the expression dog PDPN (dPDPN) canine association between dPDPN has not been elucidated. We identified that 11 out 18 tumors expressed dPDPN. Furthermore, 80% malignant melanoma (MM), squamous cell carcinoma, meningioma...
Human epidermal growth factor receptor 2 (HER2) overexpression has been reported in various human cancers. HER2-targeted therapies showed clinical responses humans with HER2-positive tumors. The incidence of canine primary lung cancer (cPLC) is increasing, but there are no effective systemic for dogs late-stage cPLC. therapy could be an option cPLC, HER2 expression cPLC remains unknown. We evaluated Immunohistochemical analysis revealed that 3 samples (19%) scored 3+; 8 (50%), 2+; 5 (31%);...
Regulatory T cells may serve as targets in cancer immunotherapy. A previous study showed that the chemokine CCL17 and receptor CCR4 play roles regulatory cell recruitment canine urothelial carcinoma. In this article, we show BRAF V595E mutation is associated with tumor-produced infiltration dogs comparison healthy dogs, carcinoma increased mRNA expression bladder elevated protein concentration urine. Immunohistochemistry levels of Foxp3 + tumor tissues The density was positively correlated...
Podoplanin (PDPN) is a prognostic factor and involved in several mechanisms of tumor progression human squamous cell carcinoma (SCC). Canine non-tonsillar SCC (NTSCC) common oral dogs has highly invasive characteristic. In this study, we investigated the function PDPN canine NTSCC. NTSCC clinical samples, overexpression was observed 80% with NTSCC, expression related to ki67 expression. knocked-out cells, proliferation, cancer stemness, migration were suppressed. As mechanism PDPN-mediated...
The human epidermal growth factor receptor 2 (HER2) is expressed in various cancers including thyroid (TC) and used as a diagnostic marker therapeutic target. Canine TC (cTC), the most common endocrine malignancy dogs, shows high metastasis rate, HER2-targeted therapy could be candidate for treatment. Here, we immunohistochemically evaluated HER2 expression 21 paraffin-embedded cTC tissues scored degree of based on intensity positivity (score: 0-3+). Four samples (19%) 3+; 6 (29%), 2+; 7...
K-ras mutation in sputum was examined using mutant-allele-specific amplification method among 100 primary lung cancer and 15 non-oncological patients. detected 11 out of 59 adenocarcinoma cases (18.6%), 5 32 squamous cell carcinoma (15.6%), 2 4 large (50.0%) 3 disease (20.0%). In the 18 both resected specimen lesion. without Therefore, these findings suggested that may not be directly related to
Podoplanin is expressed in various human tumors where it promotes tumor progression, epithelial-mesenchymal transition, and distant metastasis. also cancer-associated fibroblasts induces malignancy. The objective of this study was to evaluate podoplanin expression types feline tissues. Immunohistochemical analysis revealed that cells 13/15 (87%) squamous cell carcinomas 5/19 (26%) fibrosarcomas. Moreover, most types, including 18/21 (86%) mammary adenocarcinoma Our findings demonstrate...
Abstract Urothelial carcinoma (UC) is the most common malignancy of urinary tract in dogs and has aggressive behaviour. Although human epidermal growth factor receptor 2 (HER2) a known therapeutic target with evidence canine UC, efficacy anti‐HER2 antibody drugs remains unknown. This study aimed to investigate effects including trastuzumab emtansine (T‐DM1) on UC cell lines vitro vivo. Four (Nene, TCCUB, Love, Sora) were used. In western blotting, HER2 protein expression was observed all...
Canine urothelial carcinoma (cUC) is one of the most malignant tumors affecting dogs; however, its proliferative mechanism yet to be fully elucidated. The ubiquitin-proteasome system (UPS) an important metabolic pathway regulating protein degradation, and dysfunction leads apoptosis. We investigated antitumor effect proteasome inhibitor bortezomib, which blocks UPS. Bortezomib inhibited cell growth in cUC lines by inducing apoptosis vitro. These findings suggest potential bortezomib as a...
A comprehensive understanding of the tumour immune microenvironment (TIME) is essential for advancing precision medicine and identifying potential therapeutic targets. This study focused on canine urothelial carcinoma (cUC) recognised its high sensitivity to cyclooxygenase (COX) inhibitors. Using immunohistochemical techniques, we quantified infiltration seven cell populations within cUC tissue identify clinicopathological features that characterise TIME in cUC. Our results revealed several...
<p>Supplementary Materials and Methods</p>
<p>PDPN promotes distant colonization.</p>
<p>ROCK inhibition suppresses cell proliferation, survival, and migration.</p>
<p>PDPN regulates canine mucosal melanoma proliferation, survival, migration, and invasion.</p>
<p>Canine mucosal melanomas with PDPN high expression demonstrate enrichment of gene signatures associated amoeboid invasion.</p>
<p>Canine mucosal melanomas with PDPN high expression demonstrate enrichment of gene signatures associated amoeboid invasion.</p>
<p>PDPN promotes tumor growth and amoeboid invasion in vivo.</p>
<p>Supplementary Figure Legends 1-12</p>