A5101575277- Dermatology and Skin Diseases
- IL-33, ST2, and ILC Pathways
- T-cell and B-cell Immunology
- Pancreatic and Hepatic Oncology Research
- Asthma and respiratory diseases
- Biomedical Text Mining and Ontologies
- Gene expression and cancer classification
- Machine Learning in Bioinformatics
- Bladder and Urothelial Cancer Treatments
- Glycosylation and Glycoproteins Research
- Systemic Sclerosis and Related Diseases
- 3D Printing in Biomedical Research
- Cell Image Analysis Techniques
- Inflammatory Myopathies and Dermatomyositis
- Cancer Cells and Metastasis
- Connective Tissue Growth Factor Research
- Medicine and Dermatology Studies History
- Cancer Genomics and Diagnostics
- Tissue Engineering and Regenerative Medicine
Center for Systems Biology
2024
Harvard University
2024
Massachusetts General Hospital
2024
Brigham and Women's Hospital
2024
Center for Cancer Research
2024
Broad Institute
2024
Stanford University
2024
Johns Hopkins Medicine
2023-2024
Johns Hopkins University
2023-2024
Shanxi Medical University
2021
Abstract Prurigo nodularis (PN) is an intensely pruritic, chronic inflammatory skin disease that disproportionately affects black patients. However, the pathogenesis of PN poorly understood. We performed single-cell transcriptomic profiling, ligand receptor analysis and cell trajectory 28,695 lesional non-lesional cells to uncover disease-identifying compositions genetic characteristics. uncovered a dysregulated role for fibroblasts (FBs) myofibroblasts as key pathogenic element in PN, which...
Abstract Prurigo nodularis (PN) is a chronic inflammatory skin disease that associated with variability in peripheral blood eosinophil levels and response to T-helper 2 targeted therapies (Th2). Our objective was determine whether circulating immune profiles respect type inflammation differ by race count. Plasma from 56 PN patients 13 matched healthy controls assayed for 54 biomarkers. We compared biomarker between HCs, among based on absolute count, across racial groups PN. Eleven...
Abstract Perineural invasion (PNI), or the of cancer to space surrounding nerves, is associated with poor prognosis, but underlying molecular factors that drive its initiation and progression are poorly understood. To explore this systematically, we applied a 6000-plex spatial transcriptomic panel subcellular resolution curated microarrays from 5 pancreatic ductal adenocarcinoma (PDAC) patients spanning different stages tumor-nerve involvement. With preliminary cohort, explored malignant...
Abstract Understanding T-Cell receptor (TCR) and epitope interactions is critical for advancing our knowledge of the human immune system. Traditional approaches that use sequence similarity or structure data often struggle to scale generalize across diverse TCR/epitope interactions. To address these limitations, we introduce ImmuneCLIP, a contrastive fine-tuning method leverages pre-trained protein language models align TCR embeddings in shared latent space. ImmuneCLIP evaluated on ranking...
Abstract The aggressive nature of pancreatic ductal adenocarcinoma (PDAC) is driven by cell-intrinsic features and cell-extrinsic interactions between tumor cells the desmoplastic stroma, which infiltrated with heterogeneous populations cancer-associated-fibroblasts (CAFs) immune cells. These are able to drive emergent properties such as chemoresistance through diverse not yet fully elucidated mechanisms. Through single-nucleus RNA-seq whole-transcriptome digital spatial profiling PDAC...
Background: Prurigo nodularis (PN) is an intensely pruritic, inflammatory skin disease with a poorly understood pathogenesis. Methods: We performed single-cell transcriptomic profiling of 28,695 lesional and non-lesional PN cells. Results: Lesional has increased dysregulated fibroblasts (FBs) myofibroblasts. FBs in were shifted towards cancer-associated fibroblast (CAF)-like phenotype, POSTN+WNT5A+ CAFs PN, similarly so squamous cell carcinoma. A multi-center cohort study revealed risk SCC...
Background: Dermatomyositis (DM) is a chronic systemic autoimmune disease characterized by inflammatory infiltrates in the skin and muscle 1 . The genes pathways inflamed myopathies patients with DM are poorly understood 2 Objectives: To identify key associated further discover its pathogenesis. Methods: Muscle tissue gene expression profile (GSE143323) were acquired from GEO database, which included 39 samples 20 normal samples. differentially expressed (DEGs) screened adopting R software....