SUMMARY Several studies suggest an association between venous thromboembolism and the use of antipsychotic drugs, especially clozapine, but biological mechanisms are unknown. It has been suggested that drugs enhance aggregation platelets thereby increase risk thrombosis. The purpose present study was to examine effects clozapine its main metabolite, N ‐desmethyl as well olanzapine, risperidone haloperidol, on platelet adhesion plasma coagulation in vitro . Blood collected from healthy...

ABSTRACT Biopharmaceuticals are medical compounds derived from biological sources and often manufactured by living cells, primarily Chinese hamster ovary (CHO) cells. CHO cells display variation among cell clones, leading to growth productivity differences that influence the product's quantity quality. The environmental factors behind these not fully understood. To identify metabolites with a consistent relationship or death over time, we analyzed extracellular metabolome of 11 clones...

To determine the distribution of urinary tract pathogens with focus on Staphylococcus saprophyticus and analyse seasonality, antibiotic susceptibility, gender age distributions in a large Swedish cohort. S. is considered an important causative agent infection ( UTI ) young women, some earlier studies have reported up to approximately 40% s this patient group being caused by . We hypothesized that may be true only very specific outpatient settings. During year 2010, 113 720 urine samples were...

Background: Tissue inhibitor of metalloproteinase-1 (TIMP-1) has been suggested as a marker for abnormal regulation tissue remodelling in type 1 diabetes. Metalloproteinase-9 (MMP-9) associated with matrix turnover, and Neutrophil gelatinase lipocalin (NGAL) is tubular injury diabetic nephropathy. The aim was to analyse these biomarkers unmask early complications. Methods: Thirty-three diabetes patients, aged 20–35 years, disease duration 20 ± 5.3 years were included. Along clinical...

Extract from seeds and bark of horse chestnut (Aesculus hippocastanum L) is used as an herbal medicine against chronic venous insufficiency. The effect mechanism action on veins, arteries, platelets are not fully understood. aim this study was to investigate the effects mechanisms contraction bovine mesenteric veins human platelet aggregation. Contraction studies showed that extract dose-dependently contracted both with being most sensitive. arteries were significantly inhibited by 5-HT(2A)...

Lysophosphatidic acid (LPA) and adrenaline are weak platelet activators considered important for thrombus formation, were previously shown to synergistically increase aggregation. Here we investigate synergistic activation by LPA when measuring adhesion. Platelet-rich plasma from healthy blood donors together with and/or added protein-coated microplates. Platelets allowed adhere the amount of adhesion detected enzymatically. The combination induced a normally non-adhesive albumin surface....

Platelet adhesion is a complex and important event for prevention of blood loss after vessel injury. This study investigated fundamental adhesive mechanisms occurring in an in-vitro assay developed the measurement static human platelets plasma. The aim was to gain methodological knowledge that could be used interpretations results from other studies using this specific assay. Involvement receptors by use various antibodies as well therapeutic drugs (abciximab, eptifibatide tirofiban)....

Despite the use of anti-platelet agents such as acetylsalicylic acid (ASA) and clopidogrel in coronary heart disease, some patients continue to suffer from atherothrombosis. This has stimulated development platelet function assays monitor treatment effects. However, it is still not recommended change based on results assays. study aimed evaluate capacity a static adhesion assay detect inhibiting effects ASA clopidogrel. The measures several aspects simultaneously, which increases probability...

Abstract Background Altered mean platelet volume (MPV) and plasma albumin has been reported in type 2 diabetes (T2D). MPV is suggested to predict cardiovascular risk but there a lack of evidence for associations between adhesion. Plasma magnesium are other factors influence thrombotic risk. The objectives this study were assess the association adhesion with potential role affect activation. Methods Blood was collected from 60 T2D patients healthy controls. Platelet different protein surfaces...

Adrenaline is a platelet activator having resting plasma concentration of <1 nmol/l that increases to few during stress. However, most in vitro assays only detect effects adrenaline micromolar concentrations. This makes it difficult estimate the relevance data for vivo situation. The aim this study was investigate experimental conditions could nanomolar Platelet adhesion albumin and collagen evaluated with static assay. Our results show 10 induced platelet-rich (PRP) prepared at 140 × g,...

Contrast media (CM) are reported to possess both prothrombotic and anticoagulant properties. The mechanisms not clearly understood, early reports contradictory. To study the effects of CM on haemostasis, we analysed ex vivo ioversol iodixanol platelet adhesion P-selectin expression, in vitro ioversol, ioxaglate adhesion, expression plasma coagulation. A novel enzymatic assay was used measure protein surfaces, an enzyme-linked immunosorbent surface expression. Prothrombin time (PT) activated...

Objective: Essential thrombocythemia (ET) is a chronic myeloproliferative disorder characterized by elevated platelet counts and increased risk of thrombosis.Ex vivo data suggest reactivity in agreement with the thrombosis risk, while vitro tests often detect decreased activity.The present study aimed to investigate adhesion ET-platelets vitro, which an aspect function that has been addressed only few studies on ET patients. Material Methods:The included 30 patients 14 healthy...