A5044991129- Reproductive Biology and Fertility
- Genetics, Aging, and Longevity in Model Organisms
- MicroRNA in disease regulation
- Birth, Development, and Health
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Growth Hormone and Insulin-like Growth Factors
- Telomeres, Telomerase, and Senescence
- RNA modifications and cancer
- RNA Research and Splicing
- Molecular Biology Techniques and Applications
- COVID-19 Clinical Research Studies
- Liver physiology and pathology
- Healthcare Regulation
- Endometriosis Research and Treatment
- Extracellular vesicles in disease
- Autophagy in Disease and Therapy
- Reproductive System and Pregnancy
- Liver Disease Diagnosis and Treatment
- Macrophage Migration Inhibitory Factor
- Nutrition and Health in Aging
- Mast cells and histamine
- Childhood Cancer Survivors' Quality of Life
- Physical Education and Gymnastics
- Phagocytosis and Immune Regulation
University of Regina
2025
Universidade Estadual de Campinas (UNICAMP)
2021-2024
Oklahoma Medical Research Foundation
2022-2023
University of Central Florida
2019-2022
Universidade Federal de Pelotas
2016-2022
Cellular senescence contributes to age-related disorders including physical dysfunction, disabilities, and mortality caused by tissue inflammation damage. Senescent cells accumulate in multiple tissues with aging at etiological sites of chronic disorders. The senolytic drug combination, Dasatinib plus Quercetin (D+Q), is known reduce senescent cell abundance aged mice. However, the effects long-term D+Q treatment on intestinal inflammatory burden microbiome composition mice remain unknown....
Abstract Visceral adiposity is a risk factor for severe COVID-19, and link between adipose tissue infection disease progression has been proposed. Here we demonstrate that SARS-CoV-2 infects human undergoes productive in fat cells. However, susceptibility to the cellular response depends on anatomical origin of cells viral lineage. express more ACE2 are susceptible than their subcutaneous counterparts. leads inhibition lipolysis cells, while visceral it results higher expression...
Knowledge of protein-metabolite interactions can enhance mechanistic understanding and chemical probing biochemical processes, but the discovery endogenous ligands remains challenging. Here, we combined rapid affinity purification with precision mass spectrometry high-resolution molecular docking to precisely map physical associations 296 chemically diverse small-molecule metabolite 69 distinct essential enzymes 45 transcription factors in gram-negative bacterium Escherichia coli. We then...
The uterine fibrosis contributes to gestational outcomes. Collagen deposition in the uterus is related aging. Senolytic therapies are an option for reducing health complications We investigated effects of aging and senolytic drug combination dasatinib plus quercetin (D+Q) on fibrosis. Forty mice, 20 young females (03-months) old (18-months), were analyzed. Young (Y) (O) animals divided into groups 10 with one treatment (T) group (YT OT) another control © (YC OC). Comparative analysis...
Head and Neck Squamous Cell Carcinoma (HNSCC) is the sixth most common cancer worldwide. These tumors originate from epithelial cells of upper aerodigestive tract. HNSCC in different regions can have significantly molecular characteristics. While many microRNAs (miRNAs) been found to be involved regulation carcinogenesis pathogenesis HNSCC, new related miRNAs are still being discovered. The aim this study was explore potential miRNA biomarkers that used diagnose prognose survival patients....
Oral squamous cell carcinoma (OSCC) is the most common type of head and neck cancer and, as indicated by The Cancer Foundation, kills at an alarming rate roughly one person per hour. With this study, we aimed better understanding disease mechanisms identifying minimally invasive biomarkers profiling novel small noncoding RNAs (specifically, tRNA halves YRNA fragments) in both serum tumor tissue from humans. Small RNA-Sequencing identified multiple 5' fragments that displayed significant...
Reduced inflammation, increased insulin sensitivity, and protection against cancer are shared between humans mice with GH/IGF1 deficiency. Beyond hormone levels, miRNAs important regulators of metabolic changes associated healthy aging. We hypothesized that GH deficiency in alters the abundance circulating a subset those may overlap found GH-deficient mice. In this study, subjects untreated congenital isolated (IGHD; n = 23) control matched by age sex (n were recruited serum was collected...
Abstract The COVID-19 pandemic was initiated by the rapid spread of a SARS-CoV-2 strain. Though mainly classified as respiratory disease, infects multiple tissues throughout human body, leading to wide range symptoms in patients. To better understand how affects proteome from cells with different ontologies, this work generated an infectome atlas 9 cell models, including brain, blood, digestive system, and adipocyte tissue. Our data shows that infection trigger dysregulations on proteins...
Abstract The mammalian female is born with a limited ovarian reserve of primordial follicles. These follicles are slowly activated throughout the reproductive lifecycle, thereby determining lifecycle length. Once exhausted, women undergo menopause, which associated several metabolic perturbations and higher mortality risk. Long before exhaustion reserve, females experience severe declines in fertility health. As such, significant efforts have been made to unravel mechanisms that promote...
Crohn’s disease (CD) and rheumatoid arthritis (RA) are immune mediated inflammatory diseases. Several studies indicate a role for microRNAs (miRNAs) in the pathogenesis of variety autoimmune diseases, including CD RA. Our study’s goal was to investigate circulating miRNAs RA patients identify potential new biomarkers early detection personalized therapeutic approaches For this study, subjects with (n = 7), 8) healthy controls 7) were recruited, plasma collected miRNA sequencing. Comparison...
Estrogen signaling is protective against chronic liver diseases, although men and a subset of women are contraindicated for treatment with 17β-estradiol (17β-E2) or combination hormone replacement therapies. We sought to determine if 17α-estradiol (17α-E2), naturally occurring diastereomer 17β-E2, could attenuate fibrosis. evaluated the effects 17α-E2 on collagen synthesis degradation rates using tracer-based labeling approaches in male mice subjected carbon tetrachloride (CCl
Green juice is popularly known for introducing antioxidants, improving intestinal function and reducing weight gain.In the present study we determine antioxidant effect of green comparing it with orange juice.Rats were divided into three experimental groups submitted to supplementation 15 days: (GJ) group received juice, (OJ) control water. We evaluated activity total phenolic content juices, as well rat gain. also investigated some oxidative stress parameters, namely thiobarbituric...
Assessing cell-type-specific epigenomic and transcriptomic changes are key to understanding ovarian aging. To this end, the optimization of translating ribosome affinity purification (TRAP) method isolation nuclei tagged in specific cell types (INTACT) was performed for subsequent paired interrogation cell-specific transcriptome epigenome using a novel transgenic NuTRAP mouse model. The expression allele is under control floxed STOP cassette can be targeted promoter-specific Cre lines. Since...
The aim of this study was to investigate the effect calorie restriction (CR) during pregnancy in mice on metabolism and ovarian function offspring. Pregnant female were divided into two groups, a control group CR (n=7 each). Mice fed 50% amount consumed by females from Day 10 gestation until delivery. After weaning, offspring received diet ad libitum 3 months age, when ovaries collected. Ovaries serially cut every sixth section used for follicle counting. Female dams tended have increased...
Abstract The Ames dwarf (df/df) mouse is a well-established model for delayed aging. MicroRNAs (miRNAs), the most studied small noncoding RNAs (sncRNAs), may regulate ovarian aging to maintain younger phenotype in df/df mice. In this study, we profile other types of sncRNAs, PIWI-interacting (piRNAs) and piRNA-like (piLRNAs), young aged normal Half piRNAs derive from transfer RNA fragments (tRF-piRNAs). Aging dwarfism alter expression these novel sncRNAs. Specific tRF-piRNAs that increased...