Marios Nektarios Markakis

ORCID: 0000-0001-5328-9694
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About
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Research Areas
  • Plant Molecular Biology Research
  • melanin and skin pigmentation
  • Polysaccharides and Plant Cell Walls
  • Plant Reproductive Biology
  • Plant Stress Responses and Tolerance
  • Biochemical Analysis and Sensing Techniques
  • Plant nutrient uptake and metabolism
  • Plant responses to elevated CO2
  • RNA regulation and disease
  • Gut microbiota and health
  • Microbial Community Ecology and Physiology
  • Plant Genetic and Mutation Studies
  • Atmospheric chemistry and aerosols
  • Climate variability and models
  • Chromosomal and Genetic Variations
  • Bioeconomy and Sustainability Development
  • Natural product bioactivities and synthesis
  • Animal Genetics and Reproduction
  • 3D Printing in Biomedical Research
  • Genetic Mapping and Diversity in Plants and Animals
  • Plant-Microbe Interactions and Immunity
  • Insect and Arachnid Ecology and Behavior
  • Skin Protection and Aging
  • Microbial Metabolism and Applications
  • Transgenic Plants and Applications

University of Antwerp
2010-2022

Directorate-General for Research and Innovation
2019-2021

European Commission
2019-2021

University of Copenhagen
2016-2017

St Petersburg University
2014

Ultraviolet-B (UV-B) radiation affects leaf growth in a wide range of species. In this work, we demonstrate that UV-B levels present solar inhibit maize (Zea mays) without causing any other visible stress symptoms, including the accumulation DNA damage. We conducted kinematic analyses cell division and expansion to understand impact on these cellular processes. Our results decrease UV-B-irradiated leaves is consequence reduction production shortened zone (GZ). To determine molecular pathways...

10.1104/pp.17.00365 article EN PLANT PHYSIOLOGY 2017-04-11

The root of Arabidopsis thaliana is used as a model system to unravel the molecular nature cell elongation and its arrest. From micro-array performed on roots that were treated with aminocyclopropane-1-carboxylic acid (ACC), precursor ethylene, Small auxin-up RNA (SAUR)-like gene was found be up regulated. As it appeared 76th in family, named SAUR76. Root leaf growth overexpression lines ectopically expressing SAUR76 indicated possible involvement division process. Using promoter::GUS GFP...

10.1371/journal.pone.0082596 article EN cc-by PLoS ONE 2013-11-27

Background and Aims Plant stature shape are largely determined by cell elongation, a process that is strongly controlled at the level of wall. This associated with presence many wall proteins implicated in elongation process. Several enzyme families have been suggested to be involved weakening wall, these include xyloglucan endotransglucosylases/hydrolases (XTHs), yieldins, lipid transfer expansins. Although expansins subject much research, role involvement expansin-like genes/proteins...

10.1093/aob/mcu221 article EN Annals of Botany 2014-12-08

Summary We investigated the interaction between osmotic stress and auxin signaling in leaf growth regulation. Therefore, we grew Arabidopsis thaliana seedlings on agar media supplemented with mannitol to impose 1‐naphthaleneacetic acid (NAA), a synthetic auxin. performed kinematic analysis flow‐cytometry quantify effects cell division expansion first pair, determined homeostasis response ( DR5::β‐glucuronidase ), next‐generation sequencing transcriptome of auxin‐related mutants. Mannitol...

10.1111/nph.16490 article EN New Phytologist 2020-02-20

Abstract Background Along the root axis of Arabidopsis thaliana , cells pass through different developmental stages. In apical meristem repeated cycles division increase numbers cells. Upon leaving meristem, these transition zone where they are physiologically and mechanically prepared to undergo subsequent rapid elongation. During process elongation epidermal their length by 300% in a couple hours. When ceases, acquire final size, shape functions (in differentiation zone). Ethylene...

10.1186/1471-2229-12-208 article EN cc-by BMC Plant Biology 2012-11-07

Recently fibroblasts of many mammalian species have been reprogrammed to pluripotent state using overexpression several transcription factors. This technology allows production induced stem (iPS) cells with properties similar embryonic (ES) cells. The completeness reprogramming process is well studied in such as mouse and human but there not enough data on other species. We produced American mink (Neovison vison) ES iPS compared these transcriptome analysis. report the generation 10 22 cell...

10.1186/1471-2164-16-s13-s6 article EN cc-by BMC Genomics 2015-12-01

The synthesis and composition of cell walls is dynamically adapted in response to many developmental environmental signals. In this respect, wall proteins involved controlling elongation are critical for development. Transcriptome analysis identified a gene Arabidopsis thaliana, which was named proline-rich protein-like, AtPRPL1, based on sequence similarities from phylogenetic analysis. most resemblance found AtPRP1 AtPRP3 Arabidopsis, known be root hair growth A. thaliana four protein...

10.1093/jxb/eru308 article EN Journal of Experimental Botany 2014-08-21

In plants many developmental processes are regulated by auxin and its directional transport. PINOID (PID) kinase helps to regulate this transport influencing polar recruitment of PIN efflux proteins on the cellular membranes. We investigated how altered levels affect leaf growth in Arabidopsis thaliana. mutants with PID expression were used study effect distribution development. Single knockouts showed small pleiotropic defects. Contrastingly, several phenotypes related changes...

10.3389/fpls.2017.01009 article EN cc-by Frontiers in Plant Science 2017-06-14

Under normal or stress conditions plant growth requires a complex interplay between phytohormones and reactive oxygen species (ROS). However, details of the nature this crosstalk remain elusive. Here we demonstrate that PINOID (PID), serine threonine kinase AGC family, perturbs auxin homeostasis, which in turn modulates rosette induces responses Arabidopsis plants. mutants transgenic plants with altered PID expression were used to study effect on levels stress-related responses. In leaves...

10.3389/fpls.2017.01308 article EN cc-by Frontiers in Plant Science 2017-08-02

Although cell number generally correlates with organ size, the role of cycle control in growth regulation is still largely unsolved. We studied kip related protein (krp) 4, 6 and 7 single, double triple mutants Arabidopsis thaliana to understand inhibitory proteins leaf development. performed seed size analysis, kinematic flow cytometery, transcriptome analysis mathematical modeling G1/S G2/M checkpoint progression mitotic endoreplication cycle. Double progressively increased mature because...

10.1111/nph.15458 article EN New Phytologist 2018-09-29

Abstract Elevated CO 2 (eCO ) reduces the impact of drought, but mechanisms underlying this effect remain unclear. Therefore, we used a multidisciplinary approach to investigate interaction drought and eCO in Arabidopsis thaliana leaves. Transcriptome subsequent metabolite analyses identified strong induction aliphatic glucosinolate (GL) biosynthesis as main drought‐stressed results highlighted upregulation ABI5 downregulation WRKY6 3 transcription factors (TF), known enhance inhibit...

10.1111/pce.14521 article EN Plant Cell & Environment 2022-12-21

Abstract Growth of etiolated Arabidopsis hypocotyls is biphasic. During the first phase, cells elongate slowly and synchronously. At 48 h after imbibition, at hypocotyl base accelerate their growth. Subsequently, this rapid elongation propagates through from to top. It largely unclear what regulates switch slow fast elongation. Reverse genetics-based screening for phenotypes identified three independent mutant lines At1g70990, a short extensin (EXT) family protein that we named EXT33, with...

10.1093/pcp/pcaa049 article EN public-domain Plant and Cell Physiology 2020-04-17

Summary In a previous project, we screened the A merican mink B acterial rtificial C hromosome library, CHORI ‐231, for genes potentially involved in various coat colour phenotypes mink. Subsequently, 454 sequenced inserts containing these and developed microsatellite markers each of genes. Here, describe lack association between three different ‘roan‐type' represented by ross, S tardust innamon six that considered to be linked phenotypes. Thus, c‐ KIT ( HUGO ‐approved symbol ), ATOH ‐1 1 )...

10.1111/j.1365-2052.2012.02339.x article EN Animal Genetics 2012-03-06

Future climate scenarios indicate increasing CO2 levels in combination with more frequent drought spells. Recent studies suggest that elevated reduces the effect of stress, but mechanisms underlying reduced stress effects remain unclear. Therefore we investigated growth, physiological, biochemical and genome-wide transcriptional responses Arabidopsis thaliana to mild (MD) severe (SD) under ambient (aCO2) (eCO2), 360 620 ppm respectively. Kinematic analysis showed leaf growth by inhibiting...

10.1016/j.proenv.2015.07.233 article EN Procedia Environmental Sciences 2015-01-01
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