A5103091861- Ginseng Biological Effects and Applications
- Natural product bioactivities and synthesis
- Pharmacological Effects of Natural Compounds
- Plant biochemistry and biosynthesis
- Toxin Mechanisms and Immunotoxins
- Biological and pharmacological studies of plants
- Fungal Biology and Applications
- Biochemical and Molecular Research
- Polysaccharides and Plant Cell Walls
- Hormonal Regulation and Hypertension
- Plant Gene Expression Analysis
- Plant-Microbe Interactions and Immunity
- Plant tissue culture and regeneration
- Plant Pathogens and Fungal Diseases
- Traditional Chinese Medicine Analysis
- Drug Transport and Resistance Mechanisms
- Fungal and yeast genetics research
- Plant Pathogenic Bacteria Studies
- Gut microbiota and health
- Alzheimer's disease research and treatments
- Ziziphus Jujuba Studies and Applications
- Antioxidant Activity and Oxidative Stress
- Tryptophan and brain disorders
- Microbial Natural Products and Biosynthesis
- Diet and metabolism studies
Shanghai University of Traditional Chinese Medicine
2015-2024
Shanghai Innovative Research Center of Traditional Chinese Medicine
2023-2024
State Administration of Traditional Chinese Medicine of the People's Republic of China
2015-2024
Beijing University of Chinese Medicine
2013-2024
East China University of Science and Technology
2017-2023
Ministry of Agriculture and Rural Affairs
2022
China Agricultural University
2022
Suizhou Central Hospital
2021
Hubei University of Medicine
2021
Rensselaer Polytechnic Institute
2018-2019
A common challenge in the assembly and optimization of plant natural product biosynthetic pathways recombinant hosts is identification gene orthologues that will result best production titers. Here, we describe modular a naringenin pathway Saccharomyces cerevisiae was facilitated by optimized naringenin-inducible prokaryotic transcription activators used as biosensors. The biosensors were designed developed S. multiparametric engineering strategy, which further applied for vivo,...
Ursodeoxycholic acid (UDCA) is the main active ingredient of natural bear bile powder with multiple pharmacological functions. 7β-Hydroxysteroid dehydrogenase (HSDH) a key biocatalyst for synthesis UDCA. However, all 7β-HSDHs reported commonly suffer from poor activity and thermostability, resulting in limited productivity In this study, multiobjective directed evolution (MODE) strategy was proposed applied to improve activity, pH optimum 7β-HSDH. The best variant (V3-1) showed specific...
Prenylflavonoids are promising candidates for food additives and functional foods due to their diverse biological activities potential health benefits. However, natural prenylflavonoids generally present in low abundance limited specific plant species. Here, we report the biosynthesis of licoflavanone from naringenin prenol by recombinant
Notoginsenoside Fc (NGFc) is a protopanaxadiol-type (PPD-type) saponin from Panax notoginseng, which has perfect anti-platelet aggregatory effect. However, its pharmacokinetics and metabolism in vivo remain unknown. In this study, simple sensitive liquid chromatography/electrospray ionization tandem mass spectrometry (LC-MS/MS) method was first developed for the determination of NGFc rat plasma. After methanol-mediated protein precipitation, separation achieved on C18 column with MS...
Ginsenosides are known as the principal pharmacological active constituents in Panax medicinal plants such Asian ginseng, American and Notoginseng. Some ginsenosides, especially 20(R) isomers, found trace amounts natural sources difficult to chemically synthesize. The present study provides an approach produce ginsenosides applying biotransformation through Escherichia coli modified with relevant genes. Seven uridine diphosphate glycosyltransferase (UGT) genes originating from notoginseng,...
Inflammation is widespread in the clinical pathology and closely associated to progress of many diseases. Triterpenoid saponins as a key group active ingredients
Ginsenosides are the main bioactive ingredients in plants of genus Panax. Vina-ginsenoside R7 (VG-R7) is one rare high-value ginsenosides with health benefits. The only reported method for preparing VG-R7 involves inefficient and low-yield isolation from highly valuable natural resources. Notoginsenoside Fc (NG-Fc) isolated leaves stems Panax notoginseng a suitable substrate preparation via specific hydrolysis outside xylose at C-20 position. Here, we first screened putative enzymes...
Notoginsenoside Ft1 is a promising potential candidate for cardiovascular and cancer disease therapy owing to its positive pharmacological activities. However, the yield of ultralow utilizing reported methods. Herein, an acid hydrolyzing strategy was implemented in acquirement rare notoginsenoside Ft1. Chemical profiles were identified by ultraperformance liquid chromatography coupled with quadruple-time-of-flight electrospray ionization mass spectrometry (UPLC-Q/TOF-ESI-MS). The dynamic...
Akebia saponin D (ASD) exhibits a variety of pharmacological activities, such as anti-osteoporosis, neuroprotection, hepatoprotection, but has poor oral bioavailability. A self-nanoemulsifying drug delivery system loaded with akebia - phospholipid complex (APC-SNEDDS) (composition: Peceol: Cremophor® EL: Transcutol HP: ASD: phospholipid; ratio: 10:45:45:51:12.3, w:w:w:w:w) was first developed to improve the absorption saponins and it found significantly enhance ASD's bioavailability by 4.3...
Notoginseng Radix et Rhizoma (Sanqi), the underground part of Panax notoginseng (Burk.) F. H. Chen (Araliaceae) is commonly used in Chinese medicine for treatment haemorrhage, haemostasis, swelling, etc. The aerial including leaves, flowers and fruits are also applied similar functions. Triterpenoid saponins considered to be responsible biological activities Sanqi. Up date, more than 100 have been isolated from theroots, rhizomes, P. notoginseng. reported can classified into protopanaxadiol...
In the present study, Gentiana rigescens was screened for fungi communities to clarify their diversity and community assemblage in hosts. Meanwhile, identification activity assays of strains were also conducted. By culture-dependent (endophytic isolations from plant sections) culture-independent (metagenomic library cloning techniques, studied. The metagenomic generated using direct DNA isolation whole plants, radixes, stems, leaves, flowers soils around plant. endophytes isolated all parts...
RATIONALE 20(S)-Protopanaxadiol (PPD), a dammarane-type triterpenoid sapogenin, acts as the pharmacophore of ginsenosides which are considered principal bioactive components in Chinese ginseng. To fully understand mechanism action PPD, it is important to study its metabolic profiles vivo. METHODS Plasma, urine, fece and bile were collected after administration PPD formulated 0.5% aqueous Tween-80 rats (150 mg/kg). Samples analyzed by using sensitive reliable method based on ultra-performance...