A5068706107- Lysosomal Storage Disorders Research
- Ion channel regulation and function
- Cardiac electrophysiology and arrhythmias
- Nitric Oxide and Endothelin Effects
- Gout, Hyperuricemia, Uric Acid
- Receptor Mechanisms and Signaling
- Carbohydrate Chemistry and Synthesis
- Neuroscience and Neuropharmacology Research
- Cardiac Ischemia and Reperfusion
- Alzheimer's disease research and treatments
- Cellular transport and secretion
- Neuroscience and Neural Engineering
- Glycosylation and Glycoproteins Research
- Trypanosoma species research and implications
- Heat shock proteins research
- Cardiomyopathy and Myosin Studies
- Endoplasmic Reticulum Stress and Disease
- Protein Kinase Regulation and GTPase Signaling
- Alcohol Consumption and Health Effects
- Pharmacological Effects of Natural Compounds
- Neuroscience of respiration and sleep
- Tissue Engineering and Regenerative Medicine
- Tuberous Sclerosis Complex Research
- Case Reports on Hematomas
- Mesenchymal stem cell research
Tottori University
2015-2024
Network Group (Czechia)
2016
Evolution des Régulations Endocriniennes
2014
University of Hyogo
2011
Tokyo Medical and Dental University
2010
Shiga University of Medical Science
2010
Yamaguchi University
2010
Research Institute for Bioscience and Biotechnology
2010
Kanazawa Medical University
2010
Tottori University Hospital
2006
Amyloid beta/A4 protein precursor (APP) is secreted into medium by most cultured cells and can function as an autocrine factor. To study the biological of forms APP (sAPP) on neurons, we used a clonal CNS neuronal line, B103, which does not synthesize detectable levels APP. B103 transfected with construct developed neurites faster than parent when plated in serum-free defined medium. Neurite outgrowth was promoted conditioned APP-695-over-producing or bacteria-produced sAPP-695 (named KB75)....
Background:Uric acid (UA) serves as an antioxidant in vascular endothelial cells. UA transporter 1 (URAT1) encoded by SLC22A12 is expressed the kidney and vessels its loss of function causes hypouricemia. The purpose this study was to examine whether there any dysfunction patients with hypouricemia.Methods Results:Twenty-six hypouricemia (<2.5 mg/dl) 13 healthy control subjects were enrolled. Endothelial evaluated using flow-mediated dilation (FMD). mRNA transporters cultured human umbilical...
We investigated the mechanism of endothelin receptor type A (ETA) internalization in Chinese hamster ovary cells using two assays; flow cytometric quantification cell surface myc-ETA and <i>in situ</i> localization Cy5-labeled ET-1. In both assays, agonist-dependent was inhibited by nystatin filipin, which disrupt via caveolae, whereas it barely affected chlorpromazine hypertonic sucrose, clathrin-coated pits. addition to myc-ETA, ET-1 caused intracellular translocation caveolin-1 this also...
The growth of A-1 fibroblasts depends on exogenous amyloid beta/A4 protein precursor (APP), providing a simple bioassay to study the function APP. Our preliminary study, testing activity series fragments derived from secreted form APP-695 (sAPP-695) this bioassay, has shown that at least one active sites sAPP-695 was localized within 40-mer sequence (APP296-335, Kang sequence; Roch, J.-M., I. P. Shapiro, M. Sundsmo, D. A. C. Otero, L. Refolo, N. K. Robakis, and T. Saitoh. 1992. J. Biol....
Lysosomal β-galactosidase (β-Gal) deficiency causes a group of disorders that include neuronopathic GM1 gangliosidosis and non-neuronopathic Morquio B disease. We have previously proposed the use small molecule ligands β-Gal as pharmacological chaperones (PCs) for treatment brain pathology. Although it is still under development, PC therapy has yielded promising preclinical results in several lysosomal diseases. In this study, we evaluated effect bicyclic 1-deoxygalactonojirimycin (DGJ)...
rCAT3 (rat cationic amino acid transporter 3), a cDNA that encodes novel member of the murine CAT family was isolated. The protein encoded by contained 619 acids, 53-58% which were identical with those proteins previously described (mouse CAT1, CAT2a, CAT2b, and rat CAT1). Transient expression L-[14C]arginine incorporation experiments in COS7 cells verified high affinity system y+ activity rCAT3. First, rCAT3-mediated time-dependent saturable half-saturation constant (Km) values 103 +/- 12...
Endothelin-1 (ET-1) inhibited serum-dependent growth of asynchronized A375 human melanoma cells, and the inhibitory effect was markedly enhanced when ET-1 applied to cells synchronized at G1/S boundary by double thymidine blocks. Flow cytometric analysis revealed that did not inhibit cell cycle progression after release block but caused a significant increase hypodiploid population is characteristic apoptotic death. ET-1-induced apoptosis confirmed appearance chromatin condensation on...
We investigated intracellular trafficking of GM1 ganglioside in Niemann–Pick C1 (NPC1)-deficient Chinese hamster ovary cells [NPC1(−) cells] by using cholera toxin (CT) as a probe. Both the holotoxin and B subunit (CTB) accumulated GM1-enriched vesicles NPC1(−) cells. CTB-labeled contained early endosome marker Rab5 but not lysosome-associated membrane protein 2 were labeled with either Texas red–transferrin or Lysotracker, indicating that they represent endosomes. Similarly, CT human NPC...
Gaucher disease (GD), the most prevalent lysosomal storage disorder, is caused by mutations of beta-glucosidase (acid beta-Glu, beta-glucocerebrosidase); these result in protein misfolding. Some inhibitors this enzyme, such as iminosugar glucomimetic N-(n-nonyl)-1-deoxynojirimycin (NN-DNJ), are known to bind active site and stabilize proper folding for catalytic form, acting "chemical chaperones" that facilitate transport maturation acid beta-Glu. Recently, bicyclic nojirimycin (NJ)...
Hyperuricemia induces endothelial dysfunction, oxidative stress and inflammation, increasing cardiovascular morbidities. It also raises the incidence of atrial fibrillation; however, underlying mechanisms are unknown.The effects urate on expression Kv1.5 in cultured mouse myocytes (HL-1 cells) using reverse transcriptase-PCR, immunoblots, flow cytometry patch-clamp experiments were studied. Treatment with at 7 mg/dl for 24 h increased protein level, enhanced ultra-rapid delayed-rectifier...
<b>Background:</b> Although urate impaired the endothelial function, its underlying mechanism remains unknown. We hypothesized that nitric oxide (NO) production in human umbilical vein cells (HUVECs) via activation of uric acid transporters (UATs). <b>Purpose and method:</b> In present study, we studied effects on NO eNOS protein expression HUVEC presence absence lowering agents using molecular biological biochemical assays. <b>Results:</b> HUVECs expressed 4 kinds UATs, URATv1, ABCG2, MRP4...
Serum uric acid (UA) is taken up by endothelial cells and reduces the level of nitric oxide (NO) inhibiting its production accelerating degradation. Cytosolic plasma xanthine oxidase (XO) generates superoxide also decreases NO level. Thus, hyperuricemia associated with impaired function. Hyperuricemia often vascular diseases such as chronic kidney disease (CKD) cardiovascular (CVD). It has long been debated whether causally related to development these diseases. The 2020 American College...
Niemann-Pick type C (NPC) disease is characterized by an accumulation of cholesterol in most tissues and progressive neurodegeneration with the formation neurofibrillary tangles. Neurofibrillary tangles are composed paired helical filaments (PHF), a major component which hyperphosphorylated tau. In this study we used NPC heterozygous homozygous mouse brains to investigate molecular mechanism responsible for tauopathy NPC. Immunoblot analysis using anti-tau antibodies (Tau-1, PHF-1, AT-180,...
The endothelin (ET) family of peptides acts via two subtypes guanine nucleotide-binding regulatory protein (G protein)-coupled receptors termed ETAand ETB. ET-1 stimulated cAMP formation in Chinese hamster ovary (CHO) cells stably expressing human wild-type ETA(CHO/hETAcells) while it inhibited CHO ETB(CHO/hETBcells), and pharmacological evidence indicated that the opposite effects were due to selective coupling each receptor subtype with Gαs/Gαi. To find out a domain(s) determined coupling,...
Niemann–Pick disease type C (NPC) is an inherited lipid storage disorder caused by mutations in NPC1 or NPC2 genes. Loss of function either protein results the endosomal accumulation cholesterol and other lipids, progressive neurodegeneration, robust glial cell activation. Here, we report that cultured human NPC fibroblasts secrete interferon-β, interleukin-6 (IL-6), IL-8, contain increased levels signal transducers activators transcription (STATs). These cells also contained Toll-like...
Competitive inhibitors of either α-galactosidase (α-Gal) or β-galactosidase (β-Gal) with high affinity and selectivity have been accessed by exploiting aglycone interactions conformationally locked sp2-iminosugars. Selected compounds were profiled as potent pharmacological chaperones for mutant lysosomal α- β-Gal associated Fabry disease GM1 gangliosidosis.