A5007562144- Ion channel regulation and function
- Cardiac electrophysiology and arrhythmias
- Gout, Hyperuricemia, Uric Acid
- Receptor Mechanisms and Signaling
- Heat shock proteins research
- Inflammasome and immune disorders
- Plant tissue culture and regeneration
- Leptospirosis research and findings
- Neuroscience and Neural Engineering
- Case Reports on Hematomas
- CRISPR and Genetic Engineering
- Transgenic Plants and Applications
- Mechanical Circulatory Support Devices
- Hormonal Regulation and Hypertension
- Complement system in diseases
- Ideological and Political Education
- Cardiac Ischemia and Reperfusion
- Transplantation: Methods and Outcomes
- Adrenal and Paraganglionic Tumors
- Heme Oxygenase-1 and Carbon Monoxide
- Enterobacteriaceae and Cronobacter Research
- Inclusion and Disability in Education and Sport
- Educational Technology and Assessment
- Endometriosis Research and Treatment
- Adventure Sports and Sensation Seeking
Henan Provincial People's Hospital
2024
Tottori University
2006-2022
Guizhou University
2012-2016
Network Group (Czechia)
2016
Tianjin University
2013
Shanxi Medical University
2005
Shanxi Fenyang Hospital
2005
Institute of Crop Science
1998
ABSTRACT The replicator ( rep ) of the nopaline-type Ti plasmid pTiC58 is located adjacent to trb operon this conjugal element. Previous genetic studies region (D. R. Gallie, M. Hagiya, and C. I. Kado, J. Bacteriol. 161:1034–1041, 1985) identified functions involved in partitioning, origin replication incompatibility, copy number control. In study, we determined nucleotide sequence a 6,146-bp segment that encompasses locus pTiC58. contained four full open reading frames (ORFs) one partial...
Hyperuricemia induces endothelial dysfunction, oxidative stress and inflammation, increasing cardiovascular morbidities. It also raises the incidence of atrial fibrillation; however, underlying mechanisms are unknown.The effects urate on expression Kv1.5 in cultured mouse myocytes (HL-1 cells) using reverse transcriptase-PCR, immunoblots, flow cytometry patch-clamp experiments were studied. Treatment with at 7 mg/dl for 24 h increased protein level, enhanced ultra-rapid delayed-rectifier...
Serum uric acid (UA) is taken up by endothelial cells and reduces the level of nitric oxide (NO) inhibiting its production accelerating degradation. Cytosolic plasma xanthine oxidase (XO) generates superoxide also decreases NO level. Thus, hyperuricemia associated with impaired function. Hyperuricemia often vascular diseases such as chronic kidney disease (CKD) cardiovascular (CVD). It has long been debated whether causally related to development these diseases. The 2020 American College...
Intracellular uric acid is known to increase the protein level and channel current of atrial Kv1.5; however, mechanisms acid-induced enhancement Kv1.5 expression remain unclear. Methods Results: The effects on mRNA levels Kv1.5, as well those Akt, HSF1 Hsp70, in HL-1 cardiomyocytes were studied by using qRT-PCR Western blotting. uptake was measured radio-labeled acid. Kv1.5-mediated also patch clamp techniques. Uric up-taken cells significantly increased proteins a concentration-dependent...
ABSTRACT Conjugal transfer of pTiC58 requires two regions, tra which contains the oriT and several genes involved in DNA processing a region undefined size function that is located at 2-o’clock position plasmid. Using transposon mutagenesis with Tn 3 HoHo1 binary system, we delimited this second region, called trb , to an 11-kb interval between loci for vegetative replication nopaline catabolism. sequence analysis identified 13 significant open reading frames (ORFs) spanning 11,003 bp. The...
Abstract Objective This study aimed to explore the characteristics of carbapenem-resistant Enterobacterales (CRE) patients in intensive care unit (ICU) different regions Henan Province provide evidence for targeted prevention and treatment CRE. Methods was a cross-sectional study. CRE screening conducted ICUs 78 hospitals Province, China, on March 10, 2021. The were divided into provincial capital nonprovincial comparative analysis. Results involved 1009 total, whom 241 CRE-positive...
Human induced pluripotent stem cells (hiPSCs) harboring cardiac myosin heavy chain 6 promoter can differentiate into functional cardiomyocytes called "iCell cardiomyocytes" under blasticidin treatment condition. While iCell are expected to be used for predicting cardiotoxicity of drugs, their responses antiarrhythmic agents remain elucidated. We first examined electrophysiological properties and mRNA levels ion channels Ca handling proteins, then evaluated effects class I on Na+ Ca2+...
Long QT syndrome type 1 (LQT1) is caused by mutations in KCNQ1 coding slowly-activating delayed-rectifier K+ channels. We identified the novel missense mutation M437V of a LQT1 patient. Here, we employed iPS cell (iPSC)-derived cardiomyocytes to investigate electrophysiological properties mutant channel and cardiomyocytes.To generate iPSCs from patient healthy subject, peripheral blood T cells were reprogrammed Sendai virus vector encoding human OCT3/4, SOX2, KLF4, c-MYC. Cardiomyocytes...
Background Gout is usually found in patients with atrial fibrillation (AF). K+ efflux a common trigger of NLRP3 inflammasome activation which involved the pathogenesis AF. We investigated role channel Kv1.5 monosodium urate crystal (MSU)-induced and electrical remodeling mouse human macrophages J774.1 THP-1, myocytes HL-1. Methods Results Macrophages, primed lipopolysaccharide (LPS), were stimulated by MSU. HL-1 cells incubated conditioned medium (CM) from MSU-stimulated macrophages. Western...
Background Remote reperfusion lung injury occurs in patients with vascular occlusion and surgical procedures. Inosine monophosphate (IMP) produced by adenosine deaminase (AMPD) 3 is involved the remote injury. The purpose of present study was to identify whether IMP administration attenuated a skeletal muscle ischemia-reperfusion model. Methods Results A created using after bilateral ligation hind-limb. AMPD activity, myeloperoxidase (MPO) IMP, AMPD3 mRNA tumor necrosis factor (TNF)-α lungs...
Background:Long QT syndrome 2 (LQT2) is caused by mutations in the human ether-a-go-go-related gene (hERG). Most of its give rise to unstable hERG proteins degraded proteasome. Recently, carbachol was reported stabilize wild-type hERG-FLAG via activation muscarinic type 3 receptor (M3-mAChR). Its action on mutant hERG-FLAG, however, remains uninvestigated.
Serum uric acid level above 7 mg/dl is defined as hyperuricemia, which gives rise to the monosodium urate (MSU), causing gout and urolithiasis. Hyperuricemia an independent risk factor well a marker for hypertension, heart failure, atherosclerosis, atrial fibrillation, chronic kidney disease. MSU crystals, soluble (UA), or oxidative stress derived from xanthine oxidoreductase (XOR) might be plausible explanations association of cardio-renovascular diseases with hyperuricemia. In macrophages,...
Abstract Background The human ether‐a‐go‐go‐related gene (HERG) encodes the α‐subunit of rapidly activating delayed‐rectifier potassium channels. Mutations in this cause long QT syndrome type 2 (LQT2). In most cases, mutations reduce stability channel protein, which can be restored by heat shock (HS). Methods We identified novel mutant A78T‐HERG a patient with LQT2. purpose current study was to characterize protein and test whether HS factors (HSFs) could stabilize protein. wild‐type HERG...
Objective To investigate the expression of inducible nitric oxide synthase (iNOS) and levels (NO) in THP-1 J774A.1 cells during Leptospira interrogans (L.interrogans) infection for a better understanding mechanism macrophages involved defense against L. strains different hosts. Methods The human mononuclear (THP-1) murine (J774A.1) were infected with strain 56601. The iNOS at mRNA protein determined by using real-time RT-PCR flow cytometry analysis. NO detected Griess...