A5016820192- Plant Molecular Biology Research
- Ion Transport and Channel Regulation
- Plant Stress Responses and Tolerance
- Ion channel regulation and function
- SARS-CoV-2 and COVID-19 Research
- Magnolia and Illicium research
- Hearing, Cochlea, Tinnitus, Genetics
- Drug Transport and Resistance Mechanisms
- Photosynthetic Processes and Mechanisms
- Receptor Mechanisms and Signaling
- Plant-derived Lignans Synthesis and Bioactivity
- Natural product bioactivities and synthesis
- Cystic Fibrosis Research Advances
- Advances in Cucurbitaceae Research
- Advanced Nanomaterials in Catalysis
- Nanocluster Synthesis and Applications
- Plant-Microbe Interactions and Immunity
- Ion Channels and Receptors
- Mitochondrial Function and Pathology
- Intracerebral and Subarachnoid Hemorrhage Research
- Pharmacological Effects of Natural Compounds
- Connexins and lens biology
- Membrane-based Ion Separation Techniques
- Cardiac electrophysiology and arrhythmias
- Plant Gene Expression Analysis
Mianyang Normal University
2021-2025
Jiangsu Academy of Agricultural Sciences
2025
Institute of Pomology
2025
Westlake University
2020-2024
Wuhan University
2024
Renmin Hospital of Wuhan University
2024
State Key Laboratory of Natural Medicine
2024
China Pharmaceutical University
2018-2024
Guangdong Pharmaceutical University
2021
Nantong University
2015-2018
Abstract LAT1 (SLC7A5) is one of the representative light chain proteins heteromeric amino acid transporters, forming a heterodimer with its heavy partner 4F2hc (SLC3A2). overexpressed in many types tumors and mediates transfer drugs hormones across blood-brain barrier. Thus, considered as drug target for cancer treatment may be exploited delivery into brain. Here, we synthesized three potent inhibitors human LAT1, which inhibit transport leucine IC 50 values between 100 250 nM, solved...
Abstract The human SLC26 transporter family exhibits various transport characteristics, and member SLC26A9 performs multiple roles, including acting as Cl – /HCO 3 exchangers, channels, Na + transporters. Some mutations of are correlated with abnormalities in respiration digestion systems. As a potential target colocalizing CFTR cystic fibrosis patients, is great value drug development. Here, we present cryo-EM structure the dimer at 2.6 Å resolution. A segment C-terminal end bound to entry...
Abstract Background Fruit acidity and color are important quality attributes in peaches. Although there some exceptions, blood-fleshed peaches typically have a sour taste. However, little is known about the genetic variations linking organic acid regulation Results Here, we report peach graph-based pangenome constructed from sixteen individual genome assemblies, capturing abundant structural 82.3 Mb of sequences absent reference genome. Pangenome analysis reveals long terminal repeat...
Abstract In Gram-negative bacteria, phospholipids are major components of the inner membrane and leaflet outer membrane, playing an essential role in forming unique dual-membrane barrier to exclude entry most antibiotics. Understanding mechanisms phospholipid translocation between represents one challenges surrounding bacterial homeostasis. The conserved MlaFEDB complex functions as ABC transporter drive periplasmic protein MlaC. However, mechanism remains elusive. Here we determined three...
Abstract Angiotensin-converting enzyme 2 (ACE2) is the surface receptor for SARS coronavirus (SARS-CoV), directly interacting with spike glycoprotein (S protein). ACE2 also suggested to be new (2019-nCoV), which causing a serious epidemic in China manifested severe respiratory syndrome. B 0 AT1 (SLC6A19) neutral amino acid transporter whose expression intestinal cells requires ACE2. Here we present 2.9 Å resolution cryo-EM structure of full-length human complex AT1. The complex, assembled as...
The normal development of pollen grains and the completion double fertilization in embryos are crucial for both sexual reproduction angiosperms grain production. Actin depolymerizing factor (ADF) regulates growth, development, responses to biotic abiotic stress by binding actin plants. In this study, function ZmADF1 gene was validated through bioinformatic analysis, subcellular localization, overexpression maize Arabidopsis, knockout via CRISPR/Cas9. amino acid sequence exhibited high...
SUMMARY BES1/BZR1, a kind of plant‐specific transcription factor (TF), has been reported to regulate growth, development, and stress response. However, the maize BES1/BZR1 members are still largely unknown. In this study, we investigated function regulatory mechanism ZmBES1/BZR1‐4 in regulating drought response seed development. The was localized nucleus depending on its bHLH domain showed no self‐transactivation activity. level induced by predominantly higher seeds 25 days after...
Drought stress is a primary abiotic that causes significant losses to forestry and agricultural production. Therefore, exploring drought-responsive genes their regulatory mechanism crucial for plant molecular breeding agriculture production safety. Small auxin-up RNA (SAUR) proteins are essential in growth development but show functional diversity response. In this study, the transcriptome sequencing data of Ammopiptanthus nanus seedlings revealed expression AnSAUR50 was continuously...
A label-free, fluorescence light-up detection method for T4 polynucleotide kinase activity has been developed using the split-to-intact G-quadruplex strategy.
This article demonstrates that when cultured with micromolar zinc gluconate solutions, cancerous cells readily biosynthesized<italic>in situ</italic>biocompatible nanoclusters allowing their fluorescence bio-imaging.
Carnitine palmitoyltransferase II (CPT II) deficiency is one of the most common causes fatty acid oxidation metabolism disorders. However, molecular mechanism between CPT2 gene polymorphisms and metabolic stress has not been fully clarified. We previously reported that a number patients show thermal instable phenotype compound hetero/homozygous variants CPT II. To understand disorder resulting from deficiency, present study investigated in patient fibroblasts, [c.1102 G>A (p.V368I)]...
Abstract Temperature stress restricts plant growth and development. Antifreeze protein (AFP) can improve plants antifreeze ability. In our previous study, the AnAFP gene cloned from Ammopiptanthus nanus was confirmed to be an excellent candidate enhancing cold resistance. But, shared similar structures with KnS type dehydrins including K, N S domains except ice crystal binding domain A. Here, we generated AnAFPΔA , ΔK, AnAFPΔN ΔS, transformed them into ordinary sensitive strains of E. coli...
Abstract The Solute Carrier Family 12 (SLC12) encodes electroneutral cation-chloride cotransporters (CCCs) that are fundamental in cell volume regulation and chloride homeostasis. Dysfunction of CCCs engenders abnormality renal function neuro-system development. Here we presented structure the full length human potassium-chloride co-transporter 2 (KCC2) 3 (KCC3), KCC3 mutants phosphorylation mimic (P-mimic) dephosphorylation (DP-mimic) status, complex with [(DihydroIndenyl)Oxy] Alkanoic acid...
<title>Abstract</title> Objective The purpose of this study was to identify the AP2/ERF gene family in Magnolia officinalis (M. officinalis) and examine changes expression functional annotation as M. grows. <bold>Methods</bold> transcriptome data were evaluated compared those model plant <italic>Arabidopsis thaliana</italic>. After screening, 75 distinct <italic>AP2/ERF</italic> sequences (Ho<italic>AP2/ERF</italic>1 ~ Ho<italic>AP2/ERF</italic>75) identified analyzed via bioinformatics...
There is an urgent need for cerebroprotective interventions to improve the suboptimal outcomes with intracerebral hemorrhage (ICH). Despite important role of nonmuscle myosin heavy chain IIA (NMMHC IIA) in blood-brain barrier (BBB), its function ICH remains unclear.
Magnolia officinalis (M. officinalis) thrives in temperate, elevated regions, and its desiccated bark comprises medicinal monolignol. Both abiotic biotic factors can influence the pharmacodynamic compounds of M. officinalis, which display a variety capabilities. It was goal this study to find main bioclimatic that impact amount helpful show how these metabolic pathways magnolol honokiol through actions on transcripts molecules. We assessed amounts from Baoxing (BX), Nanjiang (NJ), Xuanhan...