A5019795131- Viral Infections and Outbreaks Research
- Viral Infections and Vectors
- Virology and Viral Diseases
- Viral gastroenteritis research and epidemiology
- SARS-CoV-2 and COVID-19 Research
- Mosquito-borne diseases and control
- Virus-based gene therapy research
- Vaccine Coverage and Hesitancy
- Hepatitis B Virus Studies
- Influenza Virus Research Studies
- COVID-19 epidemiological studies
- Disaster Response and Management
- Animal Virus Infections Studies
- HIV Research and Treatment
- Viral Infections and Immunology Research
- Respiratory viral infections research
- Malaria Research and Control
- Bacillus and Francisella bacterial research
- RNA regulation and disease
- Sepsis Diagnosis and Treatment
- RNA Interference and Gene Delivery
- Vibrio bacteria research studies
- Immune Response and Inflammation
- Thermal Regulation in Medicine
- COVID-19 Digital Contact Tracing
Centre hospitalier universitaire de Québec
2017-2024
Université Laval
2017-2024
University of Saskatchewan
2021-2024
University of Manitoba
2008-2019
Public Health Agency of Canada
2013-2018
NewLink Genetics (United States)
2017
United States Army Medical Research Institute of Infectious Diseases
2017
Nova Scotia Health Authority
2017
Izaak Walton Killam Health Centre
2017
Battelle
2017
Although Zika virus (ZIKV) infection is typically self-limiting, other associated complications such as congenital birth defects and the Guillain-Barré syndrome are well described. There no approved vaccines against ZIKV infection.
Abstract Significant concerns have been raised owing to the rapid global spread of infection and disease caused by mosquito-borne Zika virus (ZIKV). Recent studies suggest that ZIKV can also be transmitted sexually, further increasing exposure risk for this virus. Associated with is a dramatic increase in cases microcephaly additional congenital abnormalities infants ZIKV-infected mothers, as well rise occurrence Guillain Barre’ syndrome infected adults. Importantly, there are no licensed...
Abstract Background Nonlive vaccine approaches that are simple to deliver and stable at room temperature or 2–8°C could be advantageous in controlling future Ebola virus (EBOV) outbreaks. Using an immunopotent DNA generates protection from lethal EBOV challenge small animals nonhuman primates, we performed a clinical study evaluate both intramuscular (IM) novel intradermal (ID) delivery. Methods Two candidates (INO-4201 INO-4202) targeting the glycoprotein (GP) were evaluated for safety,...
Abstract Zika virus (ZIKV) is an emerging pathogen causally associated with serious sequelae in fetuses, inducing fetal microcephaly and other neurodevelopment defects. ZIKV primarily transmitted by mosquitoes, but can persist human semen sperm, sexual transmission has been documented. Moreover, exposure of type-I interferon knockout mice to results severe damage the testes, epididymis sperm. Candidate vaccines have shown protective efficacy preclinical studies carried out animal models,...
<h3>BACKGROUND:</h3> The 2013–2016 Ebola virus outbreak in West Africa was the most widespread history. In response, alive attenuated recombinant vesicular stomatitis (rVSV) vaccine expressing <i>Zaire Ebolavirus</i> glycoprotein (rVSVΔG-ZEBOV-GP) evaluated humans. <h3>METHODS:</h3> a phase 1, randomized, dose-ranging, observer-blind, placebo-controlled trial, healthy adults aged 18–65 years were randomized into 4 groups of 10 to receive one 3 doses or placebo. Follow-up visits spanned 180...
To date there are no approved antiviral drugs for the treatment of Ebola virus disease (EVD). While a number candidate have shown limited efficacy in vitro and/or non-human primate studies, differences experimental methodologies make it difficult to compare their therapeutic effectiveness. Using an model Zaire replication with transcription-competent like particles (trVLPs), requiring only level 2 biosafety containment, we compared activities type I interferons (IFNs) IFN-α and IFN-ß, panel...
Synthetically engineered DNA-encoded monoclonal antibodies (DMAbs) are an in vivo platform for evaluation and delivery of human mAb to control against infectious disease. Here, we engineer DMAbs encoding potent anti-Zaire ebolavirus (EBOV) glycoprotein (GP) mAbs isolated from Ebola virus disease survivors. We demonstrate the development a IgG1 DMAb EBOV-GP mouse model. Using this approach, show that DMAb-11 DMAb-34 exhibit functional molecular profiles comparable recombinant mAb, have wide...
As nonenveloped viruses, the aquareoviruses and orthoreoviruses are unusual in their ability to induce cell-cell fusion syncytium formation. While an extraordinary family of fusion-associated small transmembrane (FAST) proteins is responsible for orthoreovirus syncytiogenesis, basis aquareovirus-induced syncytiogenesis unknown. We now report that S7 genome segment Atlantic salmon reovirus polycistronic uses a noncanonical CUG translation start codon produce 22-kDa integral membrane protein...
Abstract Background There remains an important need for prophylactic anti-Ebola virus vaccine candidates that elicit long-lasting immune responses and can be delivered to vulnerable populations are unable receive live-attenuated or viral vector vaccines. Methods We designed novel synthetic glycoprotein (EBOV-GP) DNA vaccines as a strategy expand protective breadth against diverse EBOV strains evaluated the impact of dosing route administration on protection lethal EBOV-Makona challenge in...
The emergence and ongoing evolution of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has highlighted the need for rapid vaccine development platforms that can be updated to counteract emerging variants currently circulating future coronaviruses. Here we report a "train model" subunit platform contains SARS-CoV-2 Wuhan S1 protein (the "engine") linked series flexible receptor binding domains (RBDs; "cars") derived from concern (VOCs). We demonstrate these vaccines when combined...
The reovirus fusion-associated small transmembrane (FAST) proteins are virus-encoded membrane fusion that function as dedicated cell–cell fusogens. topology of these small, single-pass orients the majority protein on distal side (i.e., inside cell). We now show ectopic expression endodomains p10, p14, and p15 FAST enhances syncytiogenesis induced by full-length proteins, both homotypically heterotypically. Results further indicate 68-residue cytoplasmic endodomain p14 (1) is endogenously...
Identifying mosquito species is a fundamental step in risk assessment and implementation of preventative strategies. Moreover, Culex pipiens the most widespread vector several regions Iran main for transmission West Nile virus (WNV). Mosquitoes were collected at 14 sites northern 2015 2016. A subset specimens was selected identification confirmation using DNA-barcoding technique. Construction phylogenetic tree showed clustering sequences into three genera: Aedes, Anopheles with individuals...
Ebola virus (EBOV) causes lethal disease in up to 90% of EBOV-infected humans. Among vaccines, only the vesicular stomatitis platform has been successful providing postexposure protection nonhuman primates. Here, we show that an adjuvanted human adenovirus serotype 5 (Ad5)–vectored vaccine (Ad5–Zaire EBOV glycoprotein) protected 67% (6 9) and 25% (1 4) cynomolgus macaques when administered 30 minutes 24 hours following challenge, respectively. The treatment also 33% rhesus 3) given at hours....
Adeno-associated viral vectors can be used as a platform for delivering biological countermeasures against pandemic and threats. We show that vector delivery of two antibody components the ZMapp product is effective in mice systemic airway challenge with mouse-adapted strain Ebola virus. This provides generic manufacturing solution overcomes some challenges associated repeated administration protective protein.
The S1 mRNA of avian reovirus is functionally tricistronic, encoding three unrelated proteins, p10, p17 and σC, from sequential, partially overlapping open reading frames (ORFs). mechanism translation initiation at the 3′-proximal σC ORF currently unknown. Transient RNA transfections using Renilla luciferase reporter constructs revealed only a modest reduction in expression upon optimization either p10 or start sites. Insertion multiple upstream AUG (uAUG) codons preferred codon sequence...