A5040888166- Blood groups and transfusion
- Erythrocyte Function and Pathophysiology
- Platelet Disorders and Treatments
- Monoclonal and Polyclonal Antibodies Research
- Immune Cell Function and Interaction
- Blood disorders and treatments
- HIV Research and Treatment
- Hemoglobinopathies and Related Disorders
- Glycosylation and Glycoproteins Research
- Blood donation and transfusion practices
- T-cell and B-cell Immunology
- Immunodeficiency and Autoimmune Disorders
- Chronic Lymphocytic Leukemia Research
- Galectins and Cancer Biology
- Neuropeptides and Animal Physiology
- Complement system in diseases
- Cell Adhesion Molecules Research
- Blood properties and coagulation
- Protein Tyrosine Phosphatases
- Viral Infections and Outbreaks Research
- Blood transfusion and management
- Hepatitis B Virus Studies
- Immune Response and Inflammation
- Cytokine Signaling Pathways and Interactions
- Viral Infections and Vectors
Canadian Blood Services
2016-2025
University of Toronto
2016-2025
St. Michael's Hospital
2006-2023
Unity Health Toronto
2022-2023
Seattle University
2016-2020
Toronto General Hospital
2008-2020
University Health Network
2009-2020
Toronto General Hospital Research Institute
2016-2020
Centre for Social Innovation
2018
Emerald Coast Science Center
2018
Regulation of neutrophil activation is critical for disease control. Neutrophil extracellular traps (NETs), which are web-like structures composed DNA and neutrophil-derived proteins, formed following pro-inflammatory signals; however, if this process uncontrolled, NETs contribute to pathogenesis, exacerbating inflammation host tissue damage
Abstract High-dose i.v. Ig (IVIG) is used to treat various autoimmune and inflammatory diseases; however, the mechanism of action remains unclear. Based on K/BxN serum transfer arthritis model in mice, IVIG suppression inflammation has been attributed a involving basophils binding highly sialylated IgG Fc DC-SIGN–expressing myeloid cells. The requirement for sialylation was examined collagen Ab-induced (CAbIA) models mice. (1–2 g/kg body weight) suppressed when given prophylactically. same...
BACKGROUND: Sialylation of the N‐linked glycan on asparagine‐297 within Fc region intravenous gammaglobulins (IVIgs) was shown to be necessary for efficacy IVIg in amelioration experimental inflammatory arthritis. To test role sialylation immune modulating therapies beyond K/BxN arthritis model, we examined sialylated compared nonsialylated ability attenuate thrombocytopenia (ITP) a mouse model that approximates clinical setting human ITP. STUDY DESIGN AND METHODS: We used published, passive...
Autoimmune hemolytic anemias (AIHAs) are conditions involving the production of antibodies against one's own red blood cells (RBCs). These can be primary with unknown cause or secondary (by association diseases infections). There several different categories AIHAs recognized according to their features in direct antiglobulin test (DAT). (1) Warm-antibody AIHA (wAIHA) exhibits a pan-reactive IgG autoantibody recognizing portion band 3 (wherein DAT may positive IgG, C3d both). Treatment...
Activation of the cellular Src tyrosine kinase depends upon dephosphorylation carboxyl-terminal inhibitory phosphorylation site. Herein we show that isolated from human platelets and Jurkat T cells is preferentially dephosphorylated at its phosphotyrosine site by SHP-1 phosphatase. The data also revealed association with in both lymphocytes capacity to phosphorylate interact NH<sub>2</sub>-terminal SH2 domain <i>in vitro</i>. Analysis activity thymocytes SHP-1-deficient motheaten viable mice...
A reagent (ZZAP) containing a mixture of 0.1 M dithiothreitol (DTT) plus 0.1% cysteine-activated papain was found to dissociate IgG immunoglobulin from red blood cells (RBC) patients having positive direct antiglobulin test (DAT) although this could not be achieved with either chemical alone. In all 67 tested, ZZAP treatment sensitized RBC reduced the strength DAT, and in 52 instances allowed for accurate Rh phenotyping using slide/rapid tube typing reagents. This included five examples...
Significance The efficacy of immunotherapy in Alzheimer’s disease is limited, partly because antibodies, administered peripherally, have poor access to the brain. Focused ultrasound (FUS) with microbubbles allows passage antibodies from blood In a mouse model disease, blood, and without FUS, reduced amyloid pathology. contrast, FUS was required deliver sufficient hippocampus effectively promote neurogenesis. Neurogenesis, regenerative process involved memory functions, impaired disease....
IN the course of a hemolytic disorder, episodes may occur during which reticulocytes virtually disappear from blood, with result that uncompensated destruction red cells leads to progressively severe anemia. "Aplastic crises" were first described in hereditary spherocytosis1 and have been observed many other states, including autoimmune anemia (AIHA).2 3 4 5 6 7 Often attributable intercurrent infection, are usually brief characterized by transient absence red-cell precursors marrow. In some...
S ummary One hundred and forty‐eight red cell alloantibodies, of specificities generally considered to be clinical significance, were studied in vitro for their ability induce phagocytosis sensitized cells by allogeneic mononuclear phagocytes. Results indicate that only 53% the alloantibodies mediated significant . The percentages each blood group system as follows: Kell, 73%; Jk a , 32%; b 67%; D, 75%; E, 60%; Fy 62%; Yt 25%; Ge, 22%; Vel, 25%. Significant was independent strength indirect...
Mutliple myeloma (MM) has one of the poorest prognoses haematological malignancies and novel therapeutic approaches are needed. Therapeutic induction p53 might be important to evaluate drugs either individually or in combination. Direct inhibition MDM2 function by an antagonist nutlin blocking proteasomal degradation a selective proteasome inhibitor velcade can stabilize activate apoptotic signaling pathway. We examined if p53-MDM2 interaction potentiate cytotoxic effects MM cell lines...
To date there are no approved antiviral drugs for the treatment of Ebola virus disease (EVD). While a number candidate have shown limited efficacy in vitro and/or non-human primate studies, differences experimental methodologies make it difficult to compare their therapeutic effectiveness. Using an model Zaire replication with transcription-competent like particles (trVLPs), requiring only level 2 biosafety containment, we compared activities type I interferons (IFNs) IFN-α and IFN-ß, panel...
Abnormal expression of homeobox genes may lead to the development leukemias, lymphomas, and solid tumors. Expression in mammary glands, however, has not been studied actively until recently. We have examined POU human breast cancer cell lines tissue samples. Using a pair degenerate primers for reverse transcription-polymerase chain reaction (RT-PCR) followed by DNA sequencing, we found that line, MCF7, expresses at least 4 gene products: OCT1, OCT2, OCT3 OCT11 (Skn-1a/i, Epoc-1). The OCT1...
We have isolated from a human thymus cDNA library clone encoding potential protein with 54% amino acid similarity to that encoded by previously identified for yeast nucleosome assembly I (NAP-I). The deduced sequence this newly cDNA, designated hNRP (human NAP-related protein), contains seven-residue nuclear localization motif, three clusters of highly acidic residues and other structural features found in various proteins implicated chromatin formation. When expressed as fusion Escherichia...
We have investigated the effect of dithiothreitol (DTT) upon Kell blood group system and other red cell antigens. All antigens studied (K, k, Kpa, Kpb, Jsa, Jsb Ku) as well Cartwright (Yta) antigen were completely denatured after treatment with DTT. The Gerbich was substantially weakened but not denatured. Jsa appear to an exquisite sensitivity DTT can be using very low concentrations (less than or equal 2 mM) whereas require much higher for their denaturation (100-200 mM). Of 38...