A5064545408- Estrogen and related hormone effects
- Metabolomics and Mass Spectrometry Studies
- Skin Protection and Aging
- Ubiquitin and proteasome pathways
- RNA modifications and cancer
- Venomous Animal Envenomation and Studies
- Transplantation: Methods and Outcomes
- Healthcare and Venom Research
- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- DNA and Nucleic Acid Chemistry
- Receptor Mechanisms and Signaling
- Cancer, Hypoxia, and Metabolism
- Peptidase Inhibition and Analysis
- Analytical Chemistry and Chromatography
- Epigenetics and DNA Methylation
- Computational Drug Discovery Methods
- Sepsis Diagnosis and Treatment
- Muscle metabolism and nutrition
- RNA regulation and disease
- Neurotransmitter Receptor Influence on Behavior
- Free Radicals and Antioxidants
- Extracellular vesicles in disease
- Pneumocystis jirovecii pneumonia detection and treatment
- Connexins and lens biology
Inserm
2013-2025
Laboratory of Fundamental and Applied Bioenergetics
2016-2025
Université Grenoble Alpes
2016-2025
Centre Hospitalier Universitaire de Grenoble
2015-2024
Centre National de la Recherche Scientifique
2012-2024
Institut polytechnique de Grenoble
2021-2024
Translational Innovation in Medicine and Complexity
2019-2022
Assistance Publique – Hôpitaux de Paris
2021
Paris-Est Sup
2021
Université Paris-Est Créteil
2021
RATIONALE The aim of this study was to demonstrate, and characterize by high‐resolution mass spectrometry that it is possible preferentially induce covalent cross‐links in peptides using high‐energy femtosecond ultraviolet (UV) laser pulses. cross‐link readily formed only when aromatic amino acids are present the peptide sequence. METHODS Three peptides, xenopsin, angiotensin I, interleukin, individually or combination, were exposed UV pulses, either alone presence spin trapping molecules,...
Protein characterization using top-down approaches emerged with advances in high-resolution mass spectrometers and increased diversity of available activation modes: collision-induced dissociation (CID), infrared multiphoton (IRMPD) electron capture (ECD), transfer (ETD). Nevertheless, are still rarely used for glycoproteins. Hence, this work summarized the capacity to improve sequence coverage glycosylation site assignment on glycoprotein Ribonuclease B (RNase B). The glycan effect protein...
Abstract —Tryptophan hydroxylase form pig brain has been purified using a method which involved sonic disintegration of whole homogenate, ammonium sulphate fractionation, hydroxylapatite column chromatography on Sephadex G‐100 or G‐200 and finally electrophoresis poly‐acrylamide gel. The enzyme was stabilized during purification by tryptophan dithiothreitol. partially molecular weight 55,000‐60,000 as measured gel‐filtration. K m the soluble 0‐4 , differed significantly from that particulate...
KRAS mutations in non-small cell lung cancer (NSCLC) are a predictor of resistance to EGFR-targeted therapies. Because approaches target RAS signaling have been unsuccessful, targeting metabolism might help develop new strategy that could overcome drug such cancer. In this study, we applied large screening quantitative proteomic analysis evidence key enzymes involved metabolic adaptations We carried out the two KRAS-mutated NSCLC lines (A549 and NCI-H460) non tumoral bronchial line (BEAS-2B)...
Skin aging is a complex process, and lot of efforts have been made to identify new specific targets that could help diagnose, prevent, treat skin aging. Several studies concerning analyzed the changes in gene expression, very few investigations performed at protein level. Moreover, none these proteomic has used global quantitative labeled offgel approach allows more accurate description phenotype. We applied such an on human primary keratinocytes obtained from sun‐nonexposed biopsies young...
During the very first period of postnatal life, tryptophan is almost entirely free in serum rats. This situation sharp contrast with well-known ability albumin to bind essential amino acid adult. Three main factors accounted for relative lack binding during early life when compared adult: (1) lower concentration albumin, protein; (2) inhibition by nonesterified fatty acids, which were at a high level young rats until weaning, and (3) decreased number available sites on defatted albimin,...
The kinetic method is a widely used approach for the determination of thermochemical data such as proton affinities (PA) and gas-phase acidities (ΔH° acid ). These are easily obtained from decompositions noncovalent heterodimers if care taken in choice method, references used, experimental conditions. Previously, several papers have focused on theoretical considerations concerning nature references. Few investigations been devoted to conditions required validate quality results. In present...
Mutations in the X-linked MECP2 gene are responsible for Rett syndrome (RTT), a severe neurological disorder. is transcriptional modulator that finely regulates expression of many genes, specifically central nervous system. Several studies have functionally linked loss astrocytes to appearance and progression RTT phenotype non-cell autonomous manner mechanisms still unknown. Here, we used primary astroglial cells from Mecp2-deficient (KO) pups identify deregulated secreted proteins. Using...
The skin is the outermost protective barrier of human body. Its role to protect against different physical, chemical, biological and environmental stressors. vast majority studies have focused on investigating effects single stressors homeostasis induction several disorders, such as cancer or ageing. On other hand, much fewer explored consequences co-exposure cells two more simultaneously, which realistic. In present study, we investigated, using mass-spectrometry-based proteomic analysis,...
The affinity of estradiol derivatives for the estrogen receptor (ER) depends strongly on nature and stereochemistry substituents in C(11) position 17beta-estradiol (I). In this work, effects 11alpha-OH-17beta-estradiol (III(alpha)) 11beta-OH-17beta-estradiol (III(beta)) were investigated using CID experiments gas-phase acidity (DeltaH degrees (acid)) determination. showed that steroids decompose via different pathways involving competitive dissociations with rate constants depending upon...
Abstract In intensive care units, COVID‐19 viral pneumonia patients (VPP) present symptoms similar to those of other with Nonviral infection (NV‐ICU). To better manage VPP, it is therefore interesting understand the molecular pathophysiology and search for biomarkers that may clarify diagnosis. The secretome being a set proteins secreted by cells in response stimuli represents an opportunity discover new biomarkers. objective this study identify secretomic signatures VPP NV‐ICU. Plasma...
Sperm contains a wealth of cell surface receptors and ion channels that are required for most its basic functions such as motility acrosome reaction. Conversely, animal venoms enriched in bioactive compounds primarily target those receptors. We hypothesized, therefore, should be rich enough sperm-modulating drug discovery program. Our objective was to demonstrate this fact by using sperm-based phenotypic screening identify positive modulators from the venom Walterinnesia aegyptia. Herein,...
Animal venoms are complex mixtures of more than 100 different compounds, including peptides, proteins, and nonprotein compounds such as lipids, carbohydrates, metal ions. In addition, the existing show a wide range molecular weights concentrations within these venoms, making separation purification procedures quite tedious. Here, we analyzed for first time by MS advantages using OFFGEL technique in venom components Egyptian Elapidae Walterinnesia aegyptia snake compared to two classical...