A5063835507- Angiogenesis and VEGF in Cancer
- Ferroptosis and cancer prognosis
- Cancer, Lipids, and Metabolism
- RNA modifications and cancer
- Cancer, Hypoxia, and Metabolism
- Cell Adhesion Molecules Research
- MicroRNA in disease regulation
- Advanced biosensing and bioanalysis techniques
- Peptidase Inhibition and Analysis
- Pancreatic and Hepatic Oncology Research
- Cancer Immunotherapy and Biomarkers
- Protein Kinase Regulation and GTPase Signaling
- Axon Guidance and Neuronal Signaling
- Lipid metabolism and disorders
- Nanoparticle-Based Drug Delivery
- RNA Interference and Gene Delivery
- Lymphatic System and Diseases
- Epigenetics and DNA Methylation
- Phagocytosis and Immune Regulation
- Cell death mechanisms and regulation
- Caveolin-1 and cellular processes
- Cellular transport and secretion
- Graphene and Nanomaterials Applications
- Histone Deacetylase Inhibitors Research
- Immune Cell Function and Interaction
Inserm
2012-2025
Assistance Publique – Hôpitaux de Paris
2021-2025
Université Paris-Est Créteil
2014-2025
Institut Mondor de Recherche Biomédicale
2021-2025
Hôpital Albert-Chenevier
2021-2024
Hôpitaux Universitaires Henri-Mondor
2023
Paris-Est Sup
2014-2022
University of Naples Federico II
2022
Laboratoire de Recherche sur la Croissance Cellulaire, la Réparation et la Régénération Tissulaires
2014-2021
Centre National de la Recherche Scientifique
2014-2021
Only a fraction of cancer patients benefits from immune checkpoint inhibitors. This may be partly due to the dense extracellular matrix (ECM) that forms barrier for T cells. Comparing five preclinical mouse tumor models with heterogeneous microenvironments, we aimed relate rate stiffening remodeling ECM architecture and determine how these features affect intratumoral cell migration. An ECM-targeted strategy, based on inhibition lysyl oxidase, was used. In vivo stiffness measurements were...
Angiogenic factors produced by tumor cells are essential for growth and metastasis. In our study, the expression of Angiopoietin-1 (ANG1) Angiopoietin-2 (ANG2) mRNA in archival human breast cancer samples 6 cell lines was investigated. Total RNA from biopsies 38 patients extracted ANG1 ANG2 measured means quantitative real-time RT-PCR (Taqman). Matching data with available clinicopathologic biochemical revealed a significant association between axillary lymph node invasion. Univariate...
During angiogenic remodeling, Ang-1, the ligand of Tie2 tyrosine kinase, is involved in vessel sprouting and stabilization through unclear effects on nascent capillaries mural cells. In our study, we hypothesized that Ang-1/Tie2 system could cross-talk with integrins, be influenced by dynamic interactions between extracellular matrix endothelial cells (ECs). Here, show α5β1 specifically sensitizes modulates receptor activation signaling, allowing EC survival at low concentrations Ang-1...
Abstract Pancreatic cancer is a highly aggressive tumor, mostly resistant to the standard treatments. Nucleolin overexpressed in cancers and its inhibition impairs tumor growth. Herein, we showed that nucleolin was human specimens of pancreatic ductal adenocarcinoma (PDAC) overall survival significantly increased patients with low levels nucleolin. The antagonist N6L strongly impaired growth primary tumors liver metastasis an orthotopic mouse model PDAC (mPDAC). Similar antitumor effect has...
// Alex Cazes 1,2 , Lucille Lopez-Delisle Konstantina Tsarovina 3 Cécile Pierre-Eugène Katleen De Preter 4 Michel Peuchmaur 5,6 André Nicolas 7 Claire Provost 8 Caroline Louis-Brennetot Romain Daveau Candy Kumps Ilaria Cascone 9 Gudrun Schleiermacher 1,2,10 Aurélie Prignon Frank Speleman Hermann Rohrer Olivier Delattre and Isabelle Janoueix-Lerosey 1 Inserm U830, 26 rue d'Ulm, 75005 Paris, France. 2 Institut Curie, Centre de Recherche, Research Group Developmental Neurobiology, Max Planck...
In the present investigation, redox-responsive-based dextran carriers were developed for controlled release of hydrophobic molecules via a reducing agent naturally in cells, namely glutathione. this sense, was modified with thiol derivative. The roles hydrophilic segments molecular self-organisation polysaccharide derivatives into nanoparticles investigated by varying average molar mass. Crosslinked thiolated particles good such as Nile red dye, which efficiently encapsulated core and then...
The emergence and evolutionary expansion of gene families implicated in cancers other severe genetic diseases is an oddity from a natural selection perspective. Here, we show that prone to deleterious mutations the human genome have been preferentially expanded by retention "ohnolog" genes two rounds whole-genome duplication (WGD) dating back onset jawed vertebrates. We further demonstrate many ohnologs suspected be dosage balanced fact indirectly mediated their susceptibility mutations....
An NRP1-independent, non-natural SEMA3A mutant isoform is a parenterally deliverable anticancer drug that normalizes the vasculature.
Autophagy plays key roles in development, oncogenesis, cardiovascular, metabolic, and neurodegenerative diseases. Hence, understanding how autophagy is regulated can reveal opportunities to modify a disease-relevant manner. Ideally, one would want functionally define regulators whose enzymatic activity potentially be modulated. Here, we describe the STK38 protein kinase (also termed NDR1) as conserved regulator of autophagy. Using bait yeast-two-hybrid screens, discovered novel binding...
Recent evidence suggesting vascular endothelial growth factor-C (VEGF-C), which is a regulator of lymphatic and development, raised the question whether this molecule could be involved in Kaposi's sarcoma (KS), strongly angiogenic inflammatory tumor often associated with infection by human immunodeficiency virus-1. This disease characterized presence core constituted three main populations "spindle" cells, having features lymphatic/vascular macrophagic/dendritic mixed macrophage-endothelial...
Endothelial cells (ECs) self-organize into capillary networks when plated on extracellular matrix. In this process, Rho GTPases-mediated cytoskeletal dynamics control cell movement and organization of cell-to-matrix cell-to-cell contacts. Time course analysis RhoA Rac1 activation matches specific morphological aspects nascent pattern. RhoA-GTP increases early during EC adhesion accumulates at sites membrane ruffling. is activated later localizes in lamellipodia contacts organized chains....
Background & AimsCancer-associated fibroblasts (CAFs) from pancreatic adenocarcinoma (PDA) present high protein synthesis rates. CAFs express the G-protein–coupled somatostatin receptor sst1. The sst1 agonist SOM230 blocks CAF protumoral features in vitro and immunocompromised mice. We have explored here therapeutic potential of SOM230, underlying mechanisms, immunocompetent models murine PDA mimicking heavy fibrotic immunosuppressive stroma observed patient tumors.MethodsLarge-scale mass...
Angiopoietin-1 can promote migration, sprouting, and survival of endothelial cells through activation different signaling pathways triggered by the Tie2 tyrosine kinase receptor. ShcA adapter proteins are targets activated kinases implicated in transmission signals to Ras/mitogen-activated protein pathway. Here we report identification an interaction between cytoplasmic domain vitro co-immunoprecipitation analysis. Stimulation endogenous with its ligand angiopoietin-1 increased association...
Objective Weibel-Palade bodies (WPBs) are endothelial cell (EC)-specific organelles formed by vWF (von Willebrand factor) polymerization and that contain the proangiogenic factor Ang-2 (angiopoietin-2). WPB exocytosis has been shown to be implicated for vascular repair inflammatory responses. Here, we investigate role of WPBs during angiogenesis vessel stabilization. Approach Results density in ECs decreased at angiogenic front retinal network development neovascularization compared with...
Abstract The ocular vasculature is critically involved in many blinding diseases and also a popular research model for the exploration of developmental pathological angiogenesis. development vessels complex, finely orchestrated sequence events, involving spatial temporal coordination hyaloid, choroidal retinal networks. Comprehensive studies tridimensional dynamics microvascular remodeling are limited by fact that preserving disposition vascular networks cumbersome using classical...
Abstract —To define a role for the angiopoietin/Tie2 system in astrocytoma angiogenesis, we examined expression of angiopoietin-1 (Ang1) and angiopoietin-2 (Ang2) these tumors by immunohistochemistry situ hybridization. Furthermore, studied vitro effects elicited glioblastoma cell–secreted Ang1 or recombinant on functions endothelial cells (ECs). Our observations astrocytomas show that stage-specific induction angiopoietins occurs is correlated with angiogenic phases different intensity. was...