Complete mitochondrial genomes (mitogenomes) of plants are valuable resources for nucleocytoplasmic interactions, plant evolution, and cytoplasmic male sterile line breeding. However, the complete assembly mitogenomes is challenging due to frequent recombination events horizontal gene transfers. Previous studies have adopted Illumina, PacBio, Nanopore sequencing data assemble mitogenomes, but poor completeness, low accuracy, high cost limit sampling capacity. Here, we present an efficient...

Cinnamomum camphora has been cultivated as an economically important tree for its medicinal and aromatic properties. Selective breeding produced plants special uses, including spice strains with characteristic flavors aromas high-potency cultivars. The molecular biology underlying terpenoid biosynthesis is still unexplored.Gas chromatography-mass spectrometry was used to analyze the differences in contents compositions of essential oil terpenoids linalool- borneol-type chemotypes C....

As one of the most ancient tree species, codon usage pattern analysis Ginkgo biloba is a useful way to understand its evolutionary and genetic mechanisms. Several studies have been conducted on angiosperms, but seldom gymnosperms. Based RNA-Seq data G. transcriptome, amount 17,579 unigenes longer than 300 bp were selected analyzed from 68,547 candidates. The tended towards more frequently use A/U-ending codons, which showed an obvious gradient progressing gymnosperms dicots monocots....

Abstract Camellia oil extracted from seeds is rich in unsaturated fatty acids and secondary metabolites beneficial to human health. However, no oil-tea tree genome has yet been published, which a major obstacle investigating the heredity improvement of trees. Here, using both Illumina PicBio sequencing technologies, we present first chromosome-level sequence species chekiangoleosa Hu. (CCH). The assembled consists 15 pseudochromosomes with size 2.73 Gb scaffold N50 185.30 Mb. At least 2.16...

Acinetobacter is ubiquitous, and it has a high species diversity complex evolutionary pattern. To elucidate the mechanism of its ability to adapt various environment, 312 genomes strains were analyzed using phylogenomic comparative genomics methods. It was revealed that genus an open pan-genome strong genome plasticity. The consists 47,500 genes, with 818 shared by all Acinetobacter, while 22,291 are unique genes. Although do not have complete glycolytic pathway directly utilize glucose as...

strains, which are often described as plant growth promoting (PGP) bacteria, ubiquitous in many environments. A total of 213 genomes strains

Accumulating evidences show that SPLs are crucial regulators of plant abiotic stress tolerance and the highly conserved module miR156/SPL appears to balance growth responses. The halophyte Tamarix chinensis is resistant salt tress. T. (TcSPLs) theirs roles in responses remain elusive.In this study, we conducted a systematic analysis TcSPLs gene family including 12 members belonging 7 groups. physicochemical properties motifs showed divergence among groups similarity each group. microRNA...

Salix L. (willows) is one of the most taxonomically complex genera flowering plants, including shrubs, tall trees, bushes, and prostrate plants. Despite high species diversity, only five mitochondrial genomes (mitogenomes) have been released in this genus. wilsonii an important ornamental economic willow tree section Wilsonia genus Salix. In study, S. mitogenome was assembled into a typical circular structure with size 711,456 bp using PacBio HiFi sequencing. A total 58 genes were annotated...

Cinnamomum camphora , a member of the Lauraceae family, is valuable aromatic and timber tree that indigenous to south China Japan. All parts have secretory cells containing different volatile chemical compounds are utilized as herbal medicines essential oils. Here, we reported complete sequencing chloroplast genome using illumina technology. The 152,570 bp in length characterized by relatively conserved quadripartite structure large single copy region 93,705 bp, small 19,093 two inverted...

Abstract The ability of a plant to form roots from its non-root tissues is ecologically advantageous during rapid adaptation changing environment. Although this biological phenomenon has been widely utilized for cuttings in many economically important agronomic and tree species, genetic developmental mechanisms have poorly understood. In study, we conducted an association analysis small RNAs, the degradome transcriptome adventitious rooting poplar softwood cuttings, which revealed that 373...

Plant AT-rich protein and zinc-binding (PLATZ) is a class of plant-specific zinc-dependent DNA-binding that binds to A/T-rich DNA sequences. PLATZ plays an important role in seed development, water tolerance, cell proliferation early plant growth. In this study, 11 GbPLATZ s were identified from the ginkgo genome with complete PLATZ-conserved domains, which represents smaller number compared angiosperms. Multi-species phylogenetic analysis showed genes conserved plants, members represented...

Abstract Genomics research of Populus deltoides , an important timber species that is widely planted worldwide, part poplar breeding. Currently, the nuclear and chloroplast genome P. have been sequenced, but its mitochondrial (mitogenome) has not reported. To further explore evolution phylogeny mitogenome I-69 was assembled using reads from Nanopore Illumina sequencing platforms found to consist 802,637 bp three circular chromosomes (336,205, 280,841, 185,591 bp) containing 58 genes (34...

Three strains of members the family Bacillaceae, which can inhibit growth some Gram-stain-positive strains, designated M4U3P1T, HD4P25T and RD4P76T, were isolated from Suaeda salsa halophytes in Baotou, Inner Mongolia, PR China. A phylogenetic analysis based on 16S rRNA gene whole genome sequences revealed that clustered with Cytobacillus luteolus YIM 93174T a similarity 98.4 %, RD4P76T shared highest Bacillus mesophilus SA4T (97.5 %). M4U3P1T genera Salipaludibacillus Alkalicoccus...

The chloroplast genome (CPG) of Pinus massoniana belonging to the genus (Pinaceae), which is a primary source turpentine, was sequenced and analyzed in terms gene rearrangements, ndh genes loss, contraction expansion short inverted repeats (IRs). P. CPG has typical quadripartite structure that includes large single copy (LSC) (65,563 bp), small (SSC) (53,230 bp) two IRs (IRa IRb, 485 bp). 108 unique were identified, including 73 protein-coding genes, 31 tRNAs, 4 rRNAs. Most 81 simple...

Ginkgo biloba L. leaves are a flavonoid resource for the pharmaceutical industry. The 3′-hydroxylase (F3′H) is key enzyme in biosynthesis pathway. However, role of F3′H and metabolism unclear. In this study, we characterized functionally analyzed ginkgo gene GbF3′H1 that encodes protein 520 amino acids. Expression profiling showed was highly expressed September. Subcellular localization occurred predominately cytoplasm. Transgenic poplars overexpressing had more red pigmentation than did...

Auxin response factors (ARFs) are important transcription (TFs) that differentially expressed in to various abiotic stresses. The roles of ARFs and small RNA-ARF pathways mediating plant growth stress responses have emerged several recent studies. However, no studies on the involvement tamarisk trees, which resistant salinity, been conducted. In this study, systematic analysis revealed 12 TcARF genes belonging five different groups Tamarix chinensis. microRNA elements miR160, belongs group I...

Ginkgo biloba L. has a unique evolutionary status. Owing to its high medicinal and ornamental value, ginkgo also recently become research hotspot. However, the large genome long juvenile period, as well lack of an effective genetic transformation system, have hindered gaining full understanding comprehensive functions genes. At present, heterologous expression genes in model plants is primary method used ginkgo-related research; however, these distant plant relatives limit reliable...

Ginkgo biloba is a dioecious species native to China with medicinally and phylogenetically important characteristics; however, genomic resources for this are limited. In study, we performed the first transcriptome sequencing kernels at five time points using Illumina paired-end sequencing. Approximately 25.08-Gb clean reads were obtained, 68,547 unigenes an average length of 870 bp generated by de novo assembly. Of these unigenes, 29,987 (43.74%) annotated in publicly available plant protein...

Abstract Melia azedarach demonstrates strong salt tolerance and thrives in harsh saline soil conditions, but the underlying mechanisms are poorly understood. In this study, we analyzed gene expression under low, medium, high salinity conditions to gain a deeper understanding of adaptation M. stress. The GO (gene ontology) analysis unveiled prominent trend: as stress intensified, greater number differentially expressed genes (DEGs) became enriched categories related metabolic processes,...

In this study, we compared the culturability of aerobic bacteria isolated from long-term oil-contaminated soils via enrichment and direct-plating methods; were cultured at 30°C or ambient temperatures. Two soil samples collected two sites in Shengli oilfield located Dongying, China. One sample (S0) was close to outlet an oil-production water treatment plant, other (S1) 500 m downstream outlet. total, 595 bacterial isolates belonging 56 genera isolated, distributed Actinobacteria, Firmicutes,...

Strains of Lysobacter, thought to play vital roles in the environment for their high enzyme production capacity, are ubiquitous various ecosystems. During an analysis bacterial diversity saline soil, a Gram-stain-negative, aerobic, chitin-degrading strain, designated SJ-36T, was isolated from saline-alkaline soil sampled at Tumd Right Banner, Inner Mongolia, PR China. Strain SJ-36T grew 4-40 °C (optimum, 30 °C), pH 5.0-10.0 7.0-8.0) and 0-6 % NaCl 1.0 %). Oxidase catalase activities were...