A5088301257- Immune cells in cancer
- Neuroinflammation and Neurodegeneration Mechanisms
- Pancreatic function and diabetes
- Advanced Electron Microscopy Techniques and Applications
- Immune Response and Inflammation
- RNA regulation and disease
- Immune Cell Function and Interaction
- Single-cell and spatial transcriptomics
- Cancer, Hypoxia, and Metabolism
- Medical Imaging Techniques and Applications
- Herpesvirus Infections and Treatments
- Advanced Fluorescence Microscopy Techniques
- Chemokine receptors and signaling
- Angiogenesis and VEGF in Cancer
- interferon and immune responses
- Estrogen and related hormone effects
- Metabolism, Diabetes, and Cancer
- Adrenal and Paraganglionic Tumors
- Pluripotent Stem Cells Research
- Hormonal Regulation and Hypertension
- Sirtuins and Resveratrol in Medicine
- Toxin Mechanisms and Immunotoxins
- RNA Research and Splicing
- Ubiquitin and proteasome pathways
Max Delbrück Center
2020-2025
Freie Universität Berlin
2022-2025
Berlin Institute of Health at Charité - Universitätsmedizin Berlin
2022-2024
Herpes simplex virus 1 (HSV-1) induces a profound host shutoff during lytic infection. The virion (
We applied the patch-seq technique to harvest transcripts from individual microglial cells cortex, hippocampus and corpus callosum of acute brain slices adult mice. After recording membrane currents with patch-clamp technique, cytoplasm was collected via pipette underwent adapted SMART-seq2 preparation subsequent sequencing. On average, 4138 genes were detected in 113 hippocampus, including microglia markers such as Tmem119 , P2ry12 Siglec-H . Comparing our dataset previously published...
<title>Abstract</title> Resolution of inflammation, including colitis, requires carefully coordinated communication between different tissues. While the role immune cells in colitis is well established, less known about epithelial to its microenvironment. Most secreted factors that comprise cellular are directly regulated by transcription factor NF-κB. However, which transcriptomes NF-κB regulates epithelium during progression and whether these exacerbate or protect from remains...
Abstract Blood vessels play a critical role in pancreatic islet health and function, yet current culture methods to generate organoids from human pluripotent stem cells (SC-islets) lack vascular component. Here, we engineered 3D vascularized SC-islet by assembling cells, primary endothelial (ECs) fibroblasts both non-perfused model microfluidic device with perfused vessels. Vasculature improved stimulus-dependent Ca 2+ influx into SC-β-cells, hallmark of β-cell function that is blunted...
ABSTRACT Selective and controlled expansion of endogenous β-cells has been pursued as a potential therapy for diabetes. Ideally, such therapies would preserve feedback control β-cell proliferation to avoid excessive an increased risk hypoglycemia. Here, we identified regulator whose inactivation results in expansion: the protein deacetylase Sirtuin 2 (SIRT2). Sirt2 deletion mice during hyperglycemia with little effect homeostatic conditions, indicating preservation mass. SIRT2 restrains...
Abstract The human adrenal gland is a complex endocrine tissue. Developmental studies on this tissue have been limited to animal models or foetus. Here, we present cell atlas analysis of the adult normal gland, combining single-nuclei RNA sequencing and spatial transcriptome data reconstruct development tumourigenesis. We identified two populations potential progenitor cells resident within cortex: adrenocortical progenitors NR2F2 + -ID1 cells, located underneath capsule, medullary SYT1...
Abstract We applied the patch-seq technique to harvest transcripts from individual microglial cells cortex, hippocampus and corpus callosum of acute brain slices adult mice. After recording membrane currents with patch-clamp technique, cytoplasm was collected via pipette underwent adapted SMART-seq2 preparation subsequent sequencing. On average, 4138 genes were detected in 113 hippocampus, including microglia markers such as Tmem119, P2ry12 Siglec-H . Comparing our dataset previously...
The human adrenal gland is a complex endocrine tissue. Studies on renewal have been limited to animal models or foetuses. Enhancing our understanding of adult homeostasis crucial for gaining insights into the pathogenesis diseases, such as adrenocortical tumours. Here, we present comprehensive cellular genomics analysis normal gland, combining single-nuclei RNA sequencing and spatial transcriptome data reconstruct homeostasis. As expected, identified primary cells various zones cortex...
Abstract T cells need to adapt their cellular metabolism for effector cell differentiation. This relies on alterations in mitochondrial physiology. Which signals and molecules regulate those remains unclear. We recently reported, that the protein TCAIM inhibits activation-induced changes morphology function thus, CD4 + formation. Using conditional knock-in (KI) knockout (KO) mice, we now show it also applies CD8 more importantly, delineate molecular processes mitochondria by which controls...
Abstract Herpes simplex virus 1 (HSV-1) installs a profound host shut-off during lytic infection. The virion ( vhs ) protein plays key role in this process by efficiently cleaving both and viral mRNAs translation-initiation-dependent manner. Furthermore, the onset of DNA replication is accompanied rapid decline transcriptional activity genome. Both mechanisms have tremendous impact on RNA expression profile infected cells. To dissect their relative contributions elucidate gene-specific...
Searchable abstracts of presentations at key conferences in endocrinology ISSN 1470-3947 (print) | 1479-6848 (online)