A5019765145- Ubiquitin and proteasome pathways
- Immune cells in cancer
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Berberine and alkaloids research
- Cancer-related gene regulation
- DNA Repair Mechanisms
- Cancer Research and Treatments
- Wnt/β-catenin signaling in development and cancer
- MicroRNA in disease regulation
- Genetics and Neurodevelopmental Disorders
- Histone Deacetylase Inhibitors Research
- Synthesis and Biological Activity
- Cancer-related molecular mechanisms research
- Hereditary Neurological Disorders
- Cell Adhesion Molecules Research
- Cancer-related Molecular Pathways
- Connective tissue disorders research
- Galectins and Cancer Biology
- RNA Research and Splicing
- Autophagy in Disease and Therapy
- Nanoparticle-Based Drug Delivery
- Endoplasmic Reticulum Stress and Disease
- Congenital limb and hand anomalies
- Mitochondrial Function and Pathology
Shandong University
2016-2025
State Key Laboratory of Reproductive Medicine
2024
Weifang People's Hospital
2024
Southern Medical University
2023
Nanfang Hospital
2023
Ministry of Education of the People's Republic of China
2015-2017
Shandong First Medical University
1994-2013
Beijing Hospital
2013
Northwestern University
2011
Cancer Research UK
2011
Molecular pathogenesis of high-grade serous ovarian carcinoma (HG-SOC) is poorly understood. Recent recognition HG-SOC precursor lesions, defined as tubal intraepithelial (STIC) in fimbria, provides a new venue for the study early genetic changes HG-SOC. Using microRNA profiling analysis, we found that miR-182 expression was significantly higher STIC than matched normal Fallopian tube. Further revealed overexpressed most cases. To test whether plays major role tumourigenesis HG-SOC,...
The AT-hook transcription factor HMGA2 is an oncogene involved in the tumorigenesis of many malignant neoplasms. overexpression common both early and late-stage high-grade ovarian serous papillary carcinoma. To test whether participates initiation cancer promotion aggressive tumor growth, we examined oncogenic properties surface epithelial (OSE) cell lines. We found that introduction was sufficient to induce OSE transformation vitro. HMGA2-mediated resulted formation xenografts nude mice. By...
Abstract Isoliensinine, liensinine and neferine are major bisbenzylisoquinoline alkaloids in the seed embryo of lotus ( Nelumbo nucifera) exhibit potential anti-cancer activity. Here, we explored effects these on triple-negative breast cancer cells found that among three isoliensinine possesses most potent cytotoxic effect, primarily by inducing apoptosis. Interestingly, showed a much lower cytotoxicity against MCF-10A, normal human epithelial cell line. Further studies could significantly...
Glioblastoma multiforme (GBM) is the most common primary malignant brain tumor and has a poor prognosis. We, here, report potent antitumor effect of berberine, an isoquinoline alkaloid, on GBM. Berberine was found to have IC50 that much lower than temozolomide in vitro U87, U251, U118 glioblastoma cells. Although previous studies showed berberine primarily exerts its anticancer by inducing cell-cycle arrest, apoptosis, autophagy, we observed cells achieved through induction cellular...
Abstract MicroRNA-155 (miR-155) was previously found involved in the development of systemic lupus erythematosus (SLE) and other autoimmune diseases inflammatory response; however, detailed mechanism miR-155 SLE is not fully understood. To explore vivo role pathogenesis SLE, miR-155–deficient Faslpr/lpr (miR-155−/−Faslpr/lpr) mice were obtained by crossing miR-155−/− mice. Clinical features such as glomerulonephritis, autoantibody levels, immune system cell populations compared between...
Abstract Many cancer drugs exert their therapeutic effect by inducing oxidative stress in the cells. Oxidative compromises cell survival inflicting lesions macromolecules like DNA. Cancer cells rely on enhanced antioxidant metabolism and increased DNA repair function to survive assault. PARP1, a protein that senses DNA-strand breaks orchestrates repair, has an important role of damage. Berberine, alkaloid compound present many herbal plants, is capable damage downregulating homologous...
Abstract Background The therapeutic potential of mesenchymal stem cells (MSCs) may be attributed partly to the secreted paracrine factors, which comprise exosomes. Exosomes are small, saucer-shaped vesicles containing miRNAs, mRNAs, and proteins. derived from human umbilical cord (hUC-MSCs) have been reported promote angiogenesis. However, efficacy exosome-based therapies is still limited both in vitro vivo. present study aimed develop a new optical manipulation approach stimulate...
CUL4A and CUL4B, which are derived from the same ancestor, CUL4, encode scaffold proteins that organize cullin-RING ubiquitin ligase (E3) complexes. Recent genetic studies have shown germ line mutation in CUL4B can cause mental retardation, short stature, other abnormalities humans. was observed to be overexpressed breast hepatocellular cancers, although no human has been reported. Although known involved a number of cellular processes, including DNA repair cell cycle regulation, little is...
Background Micronuclei (MN) in mammalian cells serve as a reliable biomarker of genomic instability and genotoxic exposure. Elevation MN is commonly observed bearing intrinsic normal exposed to agents. DNA double-strand breaks are marked by phosphorylation H2AX at serine 139 (γ-H2AX). One subclass contains massive uniform γ-H2AX signals. This study tested whether this can be induced replication stress. Principal Findings We that large proportion MN, from 20% nearly 50%, showed staining...
Zinc-fingers and homeoboxes 2 (ZHX2) represses transcription of several genes associated with liver cancer. However, little is known about the role ZHX2 in development hepatocellular carcinoma (HCC). We investigated mechanisms by which might affect proliferation HCC cells.We overexpressed knocked down cells analyzed effects on proliferation, colony formation, cell cycle. also overexpression growth HepG2.2.15 tumor xenografts nude mice. Chromatin immunoprecipitation luciferase reporter assays...
Abstract Proliferation and migration of endothelial cells (ECs) vascular smooth muscle (VSMCs) are critical processes involved in atherosclerosis. Recent studies have revealed that microRNAs (miRNAs) can be detected circulating blood with a stable form the expression profiles differ many cellular associated coronary artery disease (CAD). However, little is known about their role, especially serum-derived miRNAs, ECs VSMCs phenotype modulation during We compared miRNA expressions serum...
Cullin-RING ligases (CRLs) complexes participate in the regulation of diverse cellular processes, including cell cycle progression, transcription, signal transduction and development. Serving as scaffold protein, cullins are crucial for assembly ligase complexes, which recognize target various substrates proteosomal degradation. Mutations human CUL4B, one eight members cullin family, major causes X-linked mental retardation. We here report generation characterization Cul4b knockout mice,...
PARP inhibitors have been widely tested in clinical trials, especially for the treatment of breast cancer and ovarian cancer, were shown to be highly successful. Because primarily functions sensing repairing DNA strand breaks, therapeutic effect inhibition is generally believed attributed impaired repair. We here report that oxidative stress also increased by mediates antitumor effect. showed PARP1 expressed specimens high grade serous carcinoma its activity required unperturbed...
Psoriasis is a chronic skin disease characterized by hyperproliferation and impaired differentiation of epidermal keratinocytes accompanied increased inflammation, suggesting that molecules with antiproliferation anti-inflammatory abilities may be effective for its treatment. One the key steps in regulating cell proliferation DNA replication initiation, which relies on prereplication complex (pre-RC) assembly chromatin. CDC6 an essential regulator pre-RC eukaryotic cells, but role psoriasis...
Artesunate, a semi-synthetic derivative of arteminisin originally developed for the treatment malaria, has recently been shown to possess antitumor properties. One cytotoxic effects artesunate on cancer cells is mediated by induction oxidative stress and DNA double-strand breaks (DSBs). We report here that in addition inducing DSBs, can also downregulate RAD51 impair DSB repair ovarian cells. observed formation foci homologous recombination (HRR) were significantly reduced artesunate-treated...