A5047101054- Lymphoma Diagnosis and Treatment
- Chronic Lymphocytic Leukemia Research
- Acute Myeloid Leukemia Research
- CAR-T cell therapy research
- Hematopoietic Stem Cell Transplantation
- Viral-associated cancers and disorders
- Immune Cell Function and Interaction
- Acute Lymphoblastic Leukemia research
- Childhood Cancer Survivors' Quality of Life
- Immunodeficiency and Autoimmune Disorders
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Hematological disorders and diagnostics
- Cancer Immunotherapy and Biomarkers
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Chronic Myeloid Leukemia Treatments
- Polyomavirus and related diseases
- Tuberculosis Research and Epidemiology
- Cutaneous lymphoproliferative disorders research
- Multiple Myeloma Research and Treatments
- Pneumonia and Respiratory Infections
- Eosinophilic Disorders and Syndromes
- Graphene and Nanomaterials Applications
- Cancer Risks and Factors
- Virus-based gene therapy research
Agostino Gemelli University Polyclinic
2019-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2018-2024
Università Cattolica del Sacro Cuore
2012-2023
Polo d’Innovazione di Genomica
2017
Catholic University of America
2013
Quantitative hematologic abnormalities have been described in the first patient studies of COVID-19, disease caused by SARS-CoV-2 virus, currently responsible for a rapidly spreading pandemic, worldwide infection with high mortality rate. In severe cases, progresses, through subsequent phases, from an upper respiratory tract local and general flu-like symptoms to viral pneumonia hyperinflammatory host reaction, which leads acute distress multi-organ failure. The most common hematological...
A low count of CD4+ and CD8+ lymphocytes is a hallmark laboratory finding in the coronavirus disease 2019 (COVID-19). Using flow cytometry, we observed significantly higher CD95 (Fas) PD-1 expression on both T cells 42 COVID-19 patients when compared to controls. Higher correlated with lower counts. percentage naive events. Our results might suggest shift antigen-activated cells, expressing molecules increasing their propensity apoptosis exhaustion during infection.
CD4+ and CD8+ chimeric antigen receptor T cells (CAR-T) play different roles in the vivo anti-tumour response, but role of /CD8+ ratio among infused CAR-T has not been clearly defined yet. We analysed leftovers from anti-CD19 bags 31 patients with aggressive B-cell lymphomas. The median was 1.44, lower for brexu-cel compared to tisa-cel axi-cel. CAR+CD4+ influenced by lactate dehydrogenase levels at apheresis, age, previous treatments or peripheral blood. Patients a response 3 months after...
Abstract Vitamin D deficiency has been reported to be a negative prognostic factor in elderly patients with aggressive B‐cell lymphomas. In vitro data suggest that vitamin supplementation may enhance rituximab‐mediated cytotoxicity. We prospectively assessed 25‐hydroxyvitamin [25( OH )D] levels at diagnosis cohort of 155 lymphomas whom 128 had diffuse large lymphoma ( DLBCL ) not otherwise specified. 25( )D were deficient (<20 ng/mL) 105 (67%), insufficient (20–29 32 (21%), and normal...
The incidence, risk factors and prognostic significance of extramedullary involvement (EMI) in adult patients with acute myeloid leukemia have not been established yet. This study analyzed the clinical biological characteristics, impact on prognosis cumulative incidence EMI a monocentric retrospective study. All consecutive pts diagnosis AML observed our institution between January 2010 December 2017 were included into analysis.Overall 346 AMLs analyzed. was 11% (38 pts). involved sites...
Abstract CD200, a transmembrane type Ia glycoprotein belonging to the immunoglobulin superfamily, has been shown have differential expression in B‐cell neoplasms. Here, we retrospectively assessed diagnostic relevance of CD200 on 427 patients with chronic neoplasms leukemic phase (median age, 69 y; range, 35‐97 y). The final diagnosis based investigator's assessment was lymphocytic leukaemia (CLL) 75% cases and non‐CLL 25% cases. Sensitivity specificity for CLL (vs non‐CLL) were calculated...
Introduction The emergence of drug-resistant Mycobacterium tuberculosis (Mtb) strains has underscored the urgent need for novel therapeutic approaches. Carbon-based nanomaterials, such as graphene oxide (GO), have shown potential in anti-TB activities but suffer from significant toxicity issues. Methods This study explores differently functionalized quantum dots (GQDs) – non-functionalized, L-GQDs, aminated (NH 2 -GQDs), and carboxylated (COOH-GQDs) alone combination with standard TB drugs...
Abstract Image‐guided core needle biopsies (IG‐CNB) represent a minimally invasive approach for obtaining tissue in patients with lymphadenopathy and suspected lymphoma. Despite their utility, diagnostic challenges persist, lower efficacy compared excisional biopsies. Our study aimed to evaluate the potential utility of incorporation flow cytometry (FC) alongside immunohistochemistry (IHC) when performing IG‐CNB lymphoproliferative diseases. Analyzing 170 consecutive cases, guided by...
Abstract The B cell receptor (BCR) is critical for mature lymphomas, serving as a therapeutic target. We show that high-grade lymphomas with MYC and BCL2 rearrangements (HGBCL-DH-BCL2) predominantly exhibit immunoglobulin heavy (IGH) chain silencing, leading to BCR shutdown. IGH-silenced HGBCL-DH-BCL2 (IGHUND) differ from IGH+ counterparts in germinal center-zone programs, expression, immune infiltrate. While favor IGM/IG-Kappa IGHUND complete IGH isotype switching IG-Lambda rearrangements....
A stable, conservative variable is needed to describe the level of water-deficit stress which grapevines are subjected. In agronomic crops, a function based on amount transpirable water stored in soil has been found provide such stable functions. This initial study examined whether this approach could be used response transpiration rate and leaf area development grapevines. The four-year-old (<i>Vitis vinifera</i> L. cv. Sangiovese) were growing was allowed dry over several days. Both rate,...
Graft-versus-host disease (GVHD) is the major life-threatening complication after allogeneic hematopoietic stem cell transplantation (allo-HSCT), developing in 35%-70% of all allo-HSCT recipients despite immunosuppressive prophylaxis. The recent application proteomic tools that allow screening for differentially expressed or excreted proteins body fluids could possibly identify specific biomarkers GVHD. Whole saliva highly attractive noninvasive specimen collection. In present study, we...
PE_PGRSs of Mycobacterium tuberculosis (Mtb) represent a family complex and peculiar proteins whose role function remain elusive. In this study, we investigated PE_PGRS3 PE_PGRS4, two highly homologous encoded by contiguous genes in the Mtb genome. Using gene-reporter system smegmatis (Ms) transcriptional analysis Mtb, show that PE_PGRS3, but not is specifically expressed under low phosphate concentrations. Interestingly, has unique, arginine-rich C-terminal domain unknown function....
Graphene Oxide has been proposed as a potential adjuvant to develop improved anti-TB treatment, thanks its activity in entrapping mycobacteria the extracellular compartment limiting their entry macrophages. Indeed, when administered together with linezolid, significantly enhanced bacterial killing due increased production of Reactive Oxygen Species. In this work, we evaluated toxicity and anti-mycobacterial on human peripheral blood mononuclear cells. Our data show that Oxide, different what...
Abstract Introduction This study aims to evaluate the effectiveness and reliability of utilization for clinical reporting evaluation digital images bone marrow aspirates by morphologists their comparability with classic microscopic morphological evaluation. Methods We scanned 180 consecutive needle smears using “Metafer4 VSlide” whole slide imaging (WSI) scanning system. evaluated statistical risk bias readings those performed on screen digitized medullary images. Results The cellularity was...
Flow cytometry (FCM) and quantitative PCR (qPCR) are conventional methods for assessing CAR-T expansion, while digital droplet (ddPCR) is emerging as a promising alternative. We monitored transcript expansion in 40 B-NHL patients post-infusion of products (axi-cel; tisa-cel; brexu-cel) with both His-Tag FCM ddPCR techniques. Sensitivity predictive capacity efficacy safety outcomes were analyzed compared FCM. A significant correlation between counts determined by CAR transcripts assessed (