A5101521778- Computational Drug Discovery Methods
- Hepatitis B Virus Studies
- Hepatitis C virus research
- Microbial Natural Products and Biosynthesis
- Anesthesia and Neurotoxicity Research
- Cholinesterase and Neurodegenerative Diseases
- Immunotherapy and Immune Responses
- Machine Learning in Materials Science
- Drug Transport and Resistance Mechanisms
- Cancer Immunotherapy and Biomarkers
- Neuropeptides and Animal Physiology
- Liver Disease Diagnosis and Treatment
- Virus-based gene therapy research
- Animal testing and alternatives
- Conflict Management and Negotiation
- Emotional Intelligence and Performance
- Treatment of Major Depression
- Job Satisfaction and Organizational Behavior
- SARS-CoV-2 and COVID-19 Research
- Monoclonal and Polyclonal Antibodies Research
- Autophagy in Disease and Therapy
- Pharmacogenetics and Drug Metabolism
- Chemical synthesis and alkaloids
- Mosquito-borne diseases and control
- Intensive Care Unit Cognitive Disorders
National Center for Advancing Translational Sciences
2016-2025
National Institutes of Health
2016-2025
Sun Yat-sen Memorial Hospital
2017-2024
Sun Yat-sen University
2017-2024
Northeast Agricultural University
2017-2024
Fosun Pharma (China)
2024
Zhongshan Hospital
2023-2024
Fudan University
2023-2024
State Key Laboratory of Genetic Engineering
2024
State Key Laboratory of Silkworm Genomic Biology
2023
Abstract Target-specific, mechanism-oriented in vitro assays post a promising alternative to traditional animal toxicology studies. Here we report the first comprehensive analysis of Tox21 effort, large-scale toxicity screening chemicals. We test ∼10,000 chemicals triplicates at 15 concentrations against panel nuclear receptor and stress response pathway assays, producing more than 50 million data points. Compound clustering by structure similarity activity profile across reveals...
Tens of thousands chemicals with poorly understood biological properties are released into the environment each day. High-throughput screening (HTS) is potentially a more efficient and cost-effective alternative to traditional toxicity tests. Using HTS, one can profile for potential adverse effects prioritize manageable number in-depth testing. Importantly, it provide clues mechanism toxicity. The Tox21 program has generated >50 million quantitative high-throughput (qHTS) data points. A...
The study investigated the relationships of five dimensions emotional intelligence: self‐awareness, self‐regulation, motivation, empathy, and social skills supervisors to subordinates' strategies handling conflict: problem solving bargaining. Data (N = 1,395) for this were collected with questionnaires from MBA students in seven countries (U.S., Greece, China, Bangladesh, Hong Kong Macau, South Africa, Portugal). Psychometric properties measures tested improved exploratory confirmatory...
<h3>Objective</h3> This study aimed to investigate the therapeutic potential of monoclonal antibody (mAb) against HBV as a novel treatment approach chronic hepatitis B (CHB) in mouse models. <h3>Methods</h3> Therapeutic effects mAbs various epitopes on viral surface protein were evaluated mice mimicking persistent infection. The immunological mechanisms mAb-mediated clearance systematically investigated. <h3>Results</h3> Among 11 tested mAbs, mAb E6F6 exhibited most striking several...
Rationale: Monoclonal antibodies (mAbs) mostly targeting extracellular or cell surface molecules have been widely used in the treatment of various diseases. However, mAbs cannot pass through membrane as efficiently small compounds, thus limiting their use against intracellular targets. Methods to shuttle into living cells may largely expand research and application areas based on mAbs. Hepatitis B virus X protein (HBx) is an important multi-functional viral life cycle hepatitis (HBV). HBx...
Computational approaches for drug discovery, such as quantitative structure–activity relationship, rely on structural similarities of small molecules to infer biological activity but are often limited identifying new candidates in the chemical spaces close known ligands. Here we report a activity-based modeling (BABM) approach, which compound profiles established across multiple assays used signatures predict other or against target. This approach was validated by candidate antivirals Zika...
It was assumed that dietary inclusion of Lactobacillus reuteri SL001 isolated from the gastric contents rabbits could act as an alternative to feed antibiotics improve growth performance broiler chickens. We randomly assigned 360 one-day-old AA white-feathered chicks in three treatments: basal diet (control), plus zinc bacitracin (antibiotic), and L. (SL001) treatment. The results showed total BW gain average daily (ADG) broilers treatment increased significantly (p < 0.05, respectively)...
Objective To examine the relationship between workplace violence (WPV) and professional identity among Chinese psychiatric nurses mediating effects of psychological capital (PsyCap) from this association. Setting Seven public tertiary hospitals in Liaoning Province, China. Participants A total 952 were recruited for study. Registered who have been engaged nursing more than 1 year eligible as participants investigation. Outcome measures Questionnaires consisting Workplace Violence Scale,...
Abstract Hepatitis B virus (HBV) X protein, HBx, interacts with anti-apoptotic Bcl-2 and Bcl-xL proteins through its BH3-like motif to promote HBV replication cytotoxicity. Here we report the crystal structure of HBx in complex where adopts a short α-helix snuggle into hydrophobic pocket via noncanonical Trp120 residue conserved Leu123 residue. This binding is ~2 Å away from canonical BH3-only structures proapoptotic proteins. Mutations altering impair vitro vivo, confirming importance this...
Kidney toxicity is a major problem both in drug development and clinical settings. It difficult to predict nephrotoxicity part because of the lack appropriate vitro cell models, limited endpoints, observation that activity membrane transporters which plays important roles by affecting pharmacokinetic profile drugs often not taken into account. We developed new model using pseudo-immortalized human primary renal proximal tubule epithelial cells. This line (SA7K) was characterized presence...
Chronic hepatitis B (CHB) patients with normal alanine aminotransferase (ALT) levels are not free from significant hepatic lesions. Recently, there has been an improved understanding of the clinical significance quantitative core antibody (qAnti-HBc) during CHB management. In this cross-sectional study, we evaluated utility qAnti-HBc in identifying liver inflammation patients.A total 469 (training set, n = 363; validation 106) who underwent biopsy (LB) were included. The quantified and...
Background Guangxi is one of the provinces having highest notification rate tuberculosis in China. However, spatial and temporal patterns association between environmental diversity are still unclear. Objective To detect spatiotemporal pattern rates from 2010 to 2016 its potential with ecological factors Zhuang autonomous region, Methods We performed a analysis prediction using time series analysis, Moran's I global local autocorrelation statistics, space-time scan statistics clusters...
About 16% of the world's population has major depressive disorder. Traditional antidepressants have slow effect rates and low response rates. Many studies shown that doses ketamine can produce rapid effective antidepressant effects. However, its mechanism action needs further exploration. Lipopolysaccharide (LPS) was used to establish a depression model in rats PC12 nerve cells were for vitro experiments. (2,4)-Dimethoxybenzylidene anabaseine dihydrochloride (GTS-21), specific agonist α7...
Inhibition of acetylcholinesterase (AChE), a biomarker organophosphorous and carbamate exposure in environmental occupational human health, has been commonly used to identify potential safety liabilities. So far, many chemicals, including drug candidates, food additives, industrial have not thoroughly evaluated for their inhibitory effects on AChE activity. inhibitors can therapeutic applications (e.g., tacrine donepezil) or neurotoxic consequences insecticides nerve agents).The objective...
The recent global pandemic of the Coronavirus disease 2019 (COVID-19) caused by new coronavirus SARS-CoV-2 presents an urgent need for development therapeutic candidates. Many efforts have been devoted to screening existing drug libraries with hope repurpose approved drugs as potential treatments COVID-19. However, antiviral mechanisms action found active in these phenotypic screens remain largely unknown. In effort deconvolute viral targets pursuit more effective anti-COVID-19 development,...
Abstract In vitro assay data have recently emerged as a potential alternative to traditional animal toxicity studies aid in the prediction of adverse effects chemicals on humans. Here we evaluate generated from battery quantitative high-throughput screening (qHTS) assays applied large and diverse collection chemicals, including approved drugs, for their capacity predicting human toxicity. Models were built with vivo data, cell-based well combination chemical structure and/or drug-target...
Natural products and their derivatives continue to be wellsprings of nascent therapeutic potential. However, many laboratories have limited resources for biological evaluation, leaving previously isolated or synthesized compounds largely completely untested. To address this issue, the Canvass library natural was assembled, in collaboration with academic industry researchers, quantitative high-throughput screening (qHTS) across a diverse set cell-based biochemical assays. Characterization...