A5100613501- Immune Cell Function and Interaction
- Cancer Immunotherapy and Biomarkers
- Immune cells in cancer
- Cell Adhesion Molecules Research
- Breast Lesions and Carcinomas
- Liver Diseases and Immunity
- Ferroptosis and cancer prognosis
- T-cell and B-cell Immunology
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Hepatitis B Virus Studies
- S100 Proteins and Annexins
- Pediatric Hepatobiliary Diseases and Treatments
- HER2/EGFR in Cancer Research
- Lung Cancer Treatments and Mutations
- Cancer Cells and Metastasis
- Metastasis and carcinoma case studies
- Bone and Dental Protein Studies
- Liver Disease Diagnosis and Treatment
- Gut microbiota and health
- Lung Cancer Research Studies
- Immunotherapy and Immune Responses
- Advanced Sensor and Energy Harvesting Materials
- CAR-T cell therapy research
- Chemokine receptors and signaling
- Muscle activation and electromyography studies
Chinese Academy of Medical Sciences & Peking Union Medical College
2025
Shanghai Ninth People's Hospital
2025
Shanghai Jiao Tong University
2025
Sun Yat-sen Memorial Hospital
2015-2024
Sun Yat-sen University
2015-2024
Hunan Cancer Hospital
2024
Tongji University
2022-2024
Shanghai Pulmonary Hospital
2022-2024
Zhejiang University
2014-2023
State Key Laboratory of Diagnosis and Treatment of Infectious Diseases
2014-2023
Trastuzumab resistance emerges to be a major issue in anti-human epidermal growth factor receptor 2 (HER2) therapy for breast cancers. Here, we demonstrated that miR-21 expression was up-regulated and its function elevated HER2(+) BT474, SKBR3, MDA-MB-453 cancer cells are induced acquire trastuzumab by long-term exposure the antibody, whereas protein of PTEN gene, target, reduced. Blocking action with antisense oligonucleotides re-sensitized resistant therapeutic activities inducing arrest,...
Abstract Macrophages play a pivotal role in tissue fibrogenesis, which underlies the pathogenesis of many end-stage chronic inflammatory diseases. MicroRNAs are key regulators immune cell functions, but their roles macrophage’s fibrogenesis have not been characterized. Here we show that IL-4 and IL-13 induce miR-142-5p downregulate miR-130a-3p macrophages; these changes sustain profibrogenic effect macrophages. In vitro , mimic prolongs STAT6 phosphorylation by targeting its negative...
Trastuzumab is a standard treatment for HER2-positive (HER2+) breast cancer, but some patients are refractory to the therapy. MicroRNAs (miRNAs) have been used predict therapeutic effects various cancers, whether miRNAs can serve as biomarkers HER2+ metastatic cancer (MBC) remains unclear. Using miRNA microarray, we identify 13 differentially expressed in serum of MBC with distinct response trastuzumab, and four selected construct signature survival using LASSO model. Further, our data show...
This study focuses on characterizing the effect of a high salt diet (HSD) intestinal immunity and risk inflammatory bowel diseases (IBD). We found that mice HSD had an increased frequency IL-17A producing cells in lamina propria (LP) compared to normal (ND). Furthermore, most were CD4+TCRβ+ cells. A LP T helper 17 (Th17) responses both small large intestines but did not increase Th17 response other gut-associated lymphoid organ. Although, change percentage regulatory (Treg) cells,...
Background/Aims: Regular physical exercise can enhance resistance to many microbial infections. However, little is known about the mechanism underlying changes in immune system induced by regular exercise. Methods: We recruited members of a university badminton club as (RE) group and healthy sedentary students control (SC) group. investigated distribution peripheral blood mononuclear cell (PBMC) subsets functions two groups. Results: There were no significant differences plasma cytokine...
Salivary Adenoid cystic carcinoma (SACC) patients with local invasion and lung metastasis are often resistant to conventional therapy such as operation, chemotherapy radiotherapy. To explore the underling mechanisms, we studied roles of miRNA in regulating invasiveness SACC cells.MicroRNA profiling was done cells microarray. MiRNA mimics or antisense oligonucleotide transfected evaluated by adhesion assay transwell assay. The target gene identified luciferase reporter "rescue" experiment....
Gut microbiota are implicated in many liver diseases. Concanavalin A (ConA)-induced hepatitis is a well-characterized murine model of fulminant immunological hepatic injury. Oral administration pathogenic bacteria or gentamycin to the mice before ConA injection, injury and lymphocyte distribution intestine were assessed. Our data show that exacerbated damage. There was more downregulation activation-induced natural killer T (NKT) cells bacteria-treated groups. Also, there negative...
Abstract Myofibroblast differentiation plays an important role in the malignant progression of phyllodes tumor, a fast-growing neoplasm derived from periductal stromal cells breast. Macrophages are frequently found close proximity with myofibroblasts, but it is uncertain whether they involved myofibroblast during tumor progression. Here we show that increased density tumor-associated macrophage (TAM) correlates tumor. We TAMs stimulated and promoted proliferation invasion cells. Furthermore,...
Abstract Glycolipids are potent activator of natural killer T (NKT) cells. The relationship between NKT cells and intestinal bacterial glycolipids in liver disorders remained unclear. We found that, sharp contrast to specific pathogen-free (SPF) mice, germ-free (GF) mice resistant Concanavalin A (ConA)-induced injury. ConA treatment failed trigger the activation hepatic GF mice. These defects correlated with sharply reduced levels CD1d-presented glycolipid antigens ConA-treated compared SPF...
To ward off a wide variety of pathogens, the human adaptive immune system harbors vast array T-cell receptors, collectively referred to as TCR repertoire. Assessment repertoire features is vital for us deeper understand behaviour and response.In this study, we used combination multiplex-PCR, Illumina sequencing IMGT (ImMunoGeneTics)/HighV-QUEST standardized analysis beta chain in blood healthy individuals, including public complementarity-determining regions (CDR3) sequences, highly expanded...
Cetuximab is a chimeric IgG1 monoclonal antibody which targets the extracellular domain of epidermal growth factor receptor. This widely used for colorectal cancer (CRC) treatment but its influence on immune system incompletely understood.The cetuximab therapy in CRC patients was investigated by analyzing peripheral blood mononuclear cells using flow cytometry. We undertook vitro cytotoxicity and cytokine-profile assays to ascertain immunomodulatory effect treatment.The number CD3+ T, CD8+...
Lung cancer has one of the highest mortalities any worldwide. Triptolide (TP) is a promising tumor suppressor extracted from Chinese herb Tripterygium wilfordii. Our previous proteomics analysis revealed that TP significantly interfered with ribosome biogenesis pathway; however, underlying molecular mechanism remains poorly understood. The aim present study was to determine TP's anticancer effect by investigating association between ribosomal stress and p53 activation. It found induces...
As there is growing evidence for the tumor microenvironment's role in tumorigenesis, we investigated of fibroblast-expressed kinases triple-negative breast cancer (TNBC). Using a high-throughput kinome screen combined with 3D invasion assays, identified PIK3Cδ (f-PIK3Cδ) as key regulator progression. Although was expressed primary fibroblasts derived from TNBC patients, it barely detectable (BC) cell lines. Genetic and pharmacological gain- loss-of-function experiments verified contribution...
The outbreak of H7N9 human infection has caused concern worldwide, but the immunological characteristics infected patients and determinants diverse outcomes remain to be thoroughly understood. In this study, twenty-three with infections were classified into severe mild cases. We found that commonly lymphopenic significantly lower levels T cells, monocytes related cytokine compared expression HLA-DR on CD14+ cells in group (in acute phase: 34.65±4.88 vs. 10.37±1.69, p<0.001). Importantly, was...
Abstract Considering the existence of immune‐desert in tumor microenvironment, clinical efficacy immunotherapy for lung adenocarcinoma is limited. This study aims to investigate ability transcription factors regulating immune microenvironment adenocarcinoma. RNA‐seq data were collected from The Cancer Genome Atlas database. relationships between and infiltrates assessed. Runt‐related factor 3 (RUNX3)‐associated pathways investigated by Kyoto Encyclopedia Genes Genomes, Gene Ontology, set...
Frailty is common among individuals with chronic kidney disease (CKD), whereas its impact on incident CKD risk remains unknown. This study aimed to prospectively evaluate the association between frailty and risk, exploring potential modification role of genetic factors (GRS). A cohort 275,442 UK Biobank participants (mean age 55.3 ± 8.1 years, 43.4% male) without were included. Physical was defined by Fried phenotypes (FP) Rockwood Index (FI). New-onset identified through hospital inpatient...
Tuberculosis (TB) remains a major public health concern worldwide. Previous studies have demonstrated that IL-17 plays an important role in initial immune response and is involved both immune-mediated protection pathology following infection with Mycobacterium tuberculosis (MTB). However, the alterations regulation of plasma level during TB treatment remain unclear. Moreover, cell type responsible for production patients requires further study. A total 20 acid-fast bacilli smear-positive...