A5014039364- Allergic Rhinitis and Sensitization
- Asthma and respiratory diseases
- Food Allergy and Anaphylaxis Research
- Monoclonal and Polyclonal Antibodies Research
- T-cell and B-cell Immunology
- Dermatology and Skin Diseases
- Contact Dermatitis and Allergies
- Advanced Biosensing Techniques and Applications
- Respiratory and Cough-Related Research
- Immune Cell Function and Interaction
- Glycosylation and Glycoproteins Research
- Blood groups and transfusion
- Immunotherapy and Immune Responses
- Antimicrobial Resistance in Staphylococcus
- Influenza Virus Research Studies
- Immunodeficiency and Autoimmune Disorders
- Bacterial Identification and Susceptibility Testing
- Immune Response and Inflammation
- Platelet Disorders and Treatments
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- vaccines and immunoinformatics approaches
- Lichen and fungal ecology
- Toxin Mechanisms and Immunotoxins
- Biotin and Related Studies
- Galectins and Cancer Biology
ALK-Abelló (Germany)
2020-2024
ALK-Abelló (Denmark)
2004-2024
Translational Research Institute
2023
Symphogen (Denmark)
2003-2015
Massachusetts General Hospital
2002
Harvard University
2002
University of Copenhagen
2000
National Institutes of Health
1999
Biotechnology Institute
1999
University of Maryland, College Park
1999
Allergen-specific immunoglobulin E (IgE) antibodies mediate pathology in diseases such as allergic rhinitis and food allergy. Memory B cells (MBCs) contribute to circulating IgE by regenerating IgE-producing plasma upon allergen encounter. Here, we report a population of type 2–polarized MBCs defined CD23 hi , IL-4Rα CD32 low at both the transcriptional surface protein levels. These MBC2s are enriched IgG1- IgG4-expressing while constitutively expressing germline transcripts for IgE. from...
Abstract Although helminths induce a polarized Th2 response they have been shown, in clinical studies, to confer protection against allergies. To elucidate the basis for this paradox, we examined influence of an enteric helminth infection on model food allergy. Upon Ag challenge, mice fed peanut (PN) extract plus mucosal adjuvant cholera toxin (CT) produced PN-specific IgE that correlated with systemic anaphylactic symptoms and elevated plasma histamine. was not induced helminth-infected PN...
The epidermal growth factor receptor (EGFR) is frequently dysregulated in human malignancies and a validated target for cancer therapy. Two monoclonal anti-EGFR antibodies (cetuximab panitumumab) are approved clinical use. However, the percentage of patients responding to treatment low many experiencing an initial response eventually relapse. Thus, need more efficacious treatments remains. Previous studies have reported that mixtures targeting multiple distinct epitopes effective than single...
Infection of host tissues by Staphylococcus aureus and S. epidermidis requires an unusual family staphylococcal adhesive proteins that contain long stretches serine-aspartate dipeptide-repeats (SDR). The prototype member this is clumping factor A (ClfA), a key virulence mediates adhesion to binding extracellular matrix such as fibrinogen. However, the biological siginificance SDR-domain its implication for pathogenesis remain poorly understood. Here, we identified two novel bacterial...
Abstract The immune system is known to generate a diverse panel of high-affinity Abs by adaptively improving the recognition pathogens during ongoing responses. In this study, we report biological limits for Ag-driven affinity maturation and repertoire diversification analyzing Ab repertoires in two adult volunteers after each three consecutive booster vaccinations with tetanus toxoid. Maturation on-rates off-rates occurred independently, indicating kinetically controlled process. third...
Abstract Allergen-specific IgE antibodies mediate allergic pathology in diseases such as rhinitis and food allergy. Memory B cells (MBCs) contribute to circulating by regenerating IgE-producing plasma upon allergen encounter. We report a population of type 2 polarized MBCs defined CD23 hi , IL-4Rα CD32 low at the transcriptional surface protein levels. These “MBC2s” are enriched IgG1 IgG4-expressing cells, while constitutively expressing germline transcripts for IgE. from patients with...
Due to technical limitations, little knowledge exists on the composition of Ag-specific polyclonal Ab responses. Hence, we here present a molecular analysis two representative human repertoires isolated by using novel single-cell cloning approach. The observed genetic diversity among tetanus toxoid-specific plasma cells indicate that are limited order 100 B cell clones and hypermutated variants thereof. Affinity kinetic binding constants log-normally distributed, median values close proposed...
Background Hypersensitivity to house dust mite (HDM) allergens is a common cause of allergic asthma symptoms and can be effectively treated with allergy immunotherapy (AIT). Objective To investigate whether genetic type 2 (T2) inflammatory biomarkers correlate disease severity in subjects asthma, this modified by AIT. Methods MITRA ( NCT01433523 ) was phase III, randomised, double-blind, placebo-controlled trial HDM sublingual (SLIT)-tablets adults asthma. Post hoc analyses the study...
House dust mite (HDM) allergens are a common cause of allergy and allergic asthma. A comprehensive analysis proteins targeted by T cells, which implicated in the development regulation disease independent their antibody reactivity, is still lacking.To comprehensively analyse HDM-derived protein targets cell responses HDM-allergic individuals, investigate correlation with IgE/IgG function.Proteomic (liquid chromatography-tandem mass spectrometry) HDM extracts identified 90 distinct clusters,...
Background: Evidence regarding sublingual immunotherapy (SLIT) efficacy and its good safety profile has been demonstrated with pollen house dust mite (HDM) allergens in the treatment of airway allergies. In addition, use grass presents a SLIT disease-modifying for respiratory Objectives: The aim this study was to demonstrate HDM-based mouse models allergic inflammation gain insights into involved local immunological mechanisms. Methods: Balb/c mice were sensitized/challenged Dermatophagoides...
Profilins are dominant pan-allergens known to cause cross-sensitization, leading clinical symptoms such as pollen-food syndrome. This study aimed determine the T-cell response Phl p 12 in profilin-sensitized patients, by measuring prevalence, strength and cross-reactivity clinically relevant profilins.The release of allergens from pollen was determined mass spectrometry immunochemistry. responses, epitope mapping profilins (Phl 12, Ole e 2, Bet v 2 Mal d 4) were measured vitro using PBMCs 26...
Background: The clinical development program of the SQ grass, ragweed, tree, and house dust mite (HDM) sublingual immunotherapy (SLIT)-tablets for allergic rhinitis/conjunctivitis (AR/C) included trials conducted in North America, Europe, Japan. Objective: Data from these were analyzed to assess efficacy, immunologic mechanisms, safety outcomes across allergens geographic regions. Methods: Thirteen phase III, double-blind, placebo controlled subjects with AR/C Europe (including Russia),...
The SQ tree SLIT-tablet (containing birch extract) proved clinically significant effects during the pollen season for as well alder/hazel. Immune outcomes of this treatment allergens from multiple homologous trees need further investigation. We hypothesize that extract AIT modulates a highly cross-reactive immune response and may be basis observed clinical cross-protection.Blood samples were collected 397 allergic patients or placebo (1:1) up to 40 weeks. Serum IgE IgG4 specific birch,...
Abstract This unit gives an introduction to the basic techniques of optical biosensing for measuring equilibrium and kinetics reversible protein interactions. Emphasis is given description simple robust approaches that will provide reliable results. How avoid most commonly encountered problems artifacts also discussed.
Many functional proteins are at least partially disordered prior to binding. Although the structural transitions upon binding of protein regions can influence affinity and specificity complexes, their precise energetic contributions unknown. Here, we use a model protein-protein interaction system in which locally region has been modified by directed evolution quantitatively assess thermodynamic disorder-to-order transitions. Through X-ray structure determination partners before after complex...
Abstract The immunogenicity of therapeutic Abs is a concern as anti-drug may impact negatively on the pharmacodynamics and safety profile Ab drugs. factors governing induction are not fully understood. In this study, we describe model based mouse-human chimeric for study in vivo. Six containing human V regions mouse C were generated from six anti-Rhesus D Ag-binding characteristics parental retained. Analysis immune response toward individual revealed anti-variable domain including...