A5100413969- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Cancer-related molecular mechanisms research
- Colorectal Cancer Treatments and Studies
- Metal complexes synthesis and properties
- Metal-Organic Frameworks: Synthesis and Applications
- Cancer Immunotherapy and Biomarkers
- Epigenetics and DNA Methylation
- RNA Research and Splicing
- Crystal structures of chemical compounds
- Genetic factors in colorectal cancer
- PARP inhibition in cancer therapy
- Radiomics and Machine Learning in Medical Imaging
- Lung Cancer Treatments and Mutations
- Lung Cancer Diagnosis and Treatment
- Magnetism in coordination complexes
- Cancer, Hypoxia, and Metabolism
- Cancer Cells and Metastasis
- CRISPR and Genetic Engineering
- Pancreatic and Hepatic Oncology Research
- Microbial metabolism and enzyme function
- Hippo pathway signaling and YAP/TAZ
- Ubiquitin and proteasome pathways
- MRI in cancer diagnosis
- Neurotransmitter Receptor Influence on Behavior
Tianjin Medical University
2019-2025
Wuhan University
2019-2025
Institute of Molecular Medicine
2025
Renmin Hospital of Wuhan University
2019-2025
Sun Yat-sen University Cancer Center
2018-2024
First Affiliated Hospital of Soochow University
2019-2024
Soochow University
2012-2024
Zhejiang University
2016-2024
Sun Yat-sen University
2012-2024
Hunan University of Traditional Chinese Medicine
2024
Small-molecule compounds approved for use as drugs may be "repurposed" new indications and studied to determine the mechanisms of their beneficial adverse effects. A comprehensive collection all small-molecule human would invaluable systematic repurposing across diseases, particularly rare neglected which cost time required development a chemical entity are often prohibitive. Previous efforts build such have been limited by complexities, redundancies, semantic inconsistencies drug naming...
Chemical genomics aims to comprehensively define, and ultimately predict, the effects of small molecule compounds on biological systems. activity profiling approaches must consider chemical all pathways operative in mammalian cells. To enable a strategic maximally efficient pathway space, we have created NCATS BioPlanet, comprehensive integrated resource that incorporates universe 1,658 human sourced from publicly available, manually curated sources, which been subjected thorough redundancy...
Abstract Metastasis is an important factor contributing to poor prognosis in patients with gastric cancer; yet, the molecular mechanism leading this cell behavior still not well understood. In study, we explored role of cysteine protease inhibitor SN (Cystatin SN, CST1) promoting cancer metastasis. We hypothesized that CST1 could regulate progression by regulating GPX4 and ferroptosis. Whole transcriptome sequencing suggested expression was significantly increased metastatic cancer, high...
Chromo/fluorophoric properties often accompany the heterocyclic scaffolds and impurities that comprise libraries used for high-throughput screening (HTS). These affect assay outputs obtained with optical detection, thus complicating analysis leading to false positives negatives. Here, we report fluorescence profile of more than 70 000 samples across spectral regions commonly utilized in HTS. The quantitative HTS paradigm was test each sample at seven or concentrations over a 4-log range...
Colorectal cancer (CRC) is the second leading cause of deaths worldwide, and metastasis principle reason for its poor prognosis. Overexpression high-mobility gene group A2 (HMGA2) contributes to aggressiveness CRC. However, underlying molecular mechanism overexpression still elusive. In this study, we showed that ectopic expression HMGA2 significantly enhanced cell migration invasion in vitro promoted tumor growth distant vivo . contrast, silencing produced opposite effects Chromatin...
Abstract Yes-associated protein (YAP) is a central player in cancer development, with functions extending beyond its recognized role cell growth regulation. Recent work has identified link between YAP/transcriptional coactivator PDZ-binding motif (TAZ) and the DNA damage response. Here, we investigated mechanistic underpinnings of cross-talk repair YAP activity. Ku70, key component nonhomologous end joining pathway to damage, engaged dynamic competition TEAD4 for binding YAP, limiting...
Clinical studies on atrial fibrillation (AF) recurrence after catheter ablation in patients diagnosed with patent foramen ovale (PFO) and paroxysmal AF (PAF) are scarce. Here, we aimed to develop a nomogram model utilizing multimodal data for the risk stratification of following individuals PFO new-onset PAF. Patients PAF who underwent at Renmin Hospital Wuhan University from January 2018 June 2020 were consecutively enrolled. The identification potential factors was conducted using...
Two titanium-oxo-clusters Ti(6)O(4)(o-BDC)(2)(o-BDC(i)Pr)(2)(O(i)Pr)(10) (1) and Ti(6)O(3)(o-BDC)(2)(O(i)Pr)(14) (2) (BDC = benzene dicarboxylate) were prepared by one-step in situ solvothermal synthesis. The compounds are the rare examples of dicarboxylate-substituted titanium-oxo-clusters. Their crystal structures successfully measured single-crystal X-ray analysis. Ti(6) oxo-clusters 1 2 constructed two dual corner-missing cube subunit, Ti(3)O(3). subunits linked double μ(3)-O bridges for...
Abstract Background Aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor associated with gastric carcinogenesis. 3,3'-Diindolylmethane (DIM) relatively non-toxic selective AhR modulator. This study was to detect the effects of DIM on cancer cell growth. Methods Gastric SGC7901 treated at different concentrations (0,10,20,30,40,50 μmol/L) or without an antagonist, resveratrol. The expression and Cytochrome P4501A1 (CYP1A1), classic target gene pathway, were detected by...
Impaired wound healing is commonly encountered in patients with diabetes mellitus, which may lead to severe outcomes such as amputation, if untreated timely. Macrophage plays a critical role the process including resolution phase. Although magnetic therapy known improve microcirculation, its effect on remains uncertain. In present study, we found that 0.6 T static field (SMF) significantly accelerated closure and elevated reepithelialization revascularization diabetic mice. Notably, SMF...
Gastric cancer is one of the cancers with high morbidity and mortality worldwide. The aryl sulfonamide indisulam inhibits proliferation several types cells through its function as a molecular glue to promote ubiquitination degradation RNA-binding motif protein 39 (RBM39). However, it unknown whether how regulates migration cells. In this work, using label-free quantitative proteomics, we discover that significantly attenuates N-cadherin, marker for epithelial mesenchymal transition Our...
Abstract Temozolomide (TMZ) therapy offers minimal clinical benefits in patients with glioblastoma multiforme (GBM) high EGFR activity, underscoring the need for effective combination therapy. Here, we show that tonicity-responsive enhancer binding protein (NFAT5) lysine methylation, is a determinant of TMZ response. Mechanistically, activation induces phosphorylated EZH2 (Ser21) and triggers NFAT5 methylation at K668. Methylation prevents cytoplasm interaction E3 ligase TRAF6, thus blocks...
LOX-like 1 (LOXL1) is a lysyl oxidase, and emerging evidence has revealed its effect on malignant cancer progression. However, role in colorectal (CRC) the underlying molecular mechanisms have not yet been elucidated.LOXL1 expression was detected by immunohistochemistry, western blotting real-time PCR. In vitro, colony formation, wound healing, migration invasion assays were performed to investigate effects of LOXL1 cell proliferation, invasion. vivo, metastasis models mouse xenografts used...
Indisulam exhibits antitumor activity against several cancer cells. Although the DCAF15-indisulam-RBM39 axis has been well documented in inhibition of cell growth, it is unknown whether RBM39 degradation alone mechanism action indisulam. Here, we verified inhibitory effect indisulam on proliferation gastric cells and its dependence DCAF15. Proximity-dependent biotin labeling with TurboID quantitative proteomics revealed that indeed promoted interaction between DCAF15 RBM39. Immunoblotting...
Currently, a substantial number of patients suffer from the distress skin defects. Tissue-engineered represents promising alternative therapeutic option for these patients. However, currently available tissue-engineered products still exhibit significant limitations, including poor long-term viability and lack appendages. The advent 3D printing technology organoid formation techniques provides avenue addressing challenges. In this study, we constructed scaffold using three-dimensional with...
Gain-of-function mutations of Notch2 cause the rare autosomal dominant disorder known as Hajdu–Cheney syndrome (HCS). Most patients with HCS develop congenital heart disease; however, precise mechanisms remain elusive. Here, a murine model expressing human intracellular domain (hN2ICD) in cardiomyocytes (hN2ICD-Tg CM ) was generated and mice spontaneously developed ventricular diastolic dysfunction preserved ejection fraction cardiac hypertrophy. Ectopic hN2ICD expression promoted...