A5100365344- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Impact of Technology on Adolescents
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- Viral Infections and Outbreaks Research
- Transgenic Plants and Applications
- Viral Infectious Diseases and Gene Expression in Insects
- Monoclonal and Polyclonal Antibodies Research
- Malaria Research and Control
- Computational Drug Discovery Methods
- Child Development and Digital Technology
- Insect symbiosis and bacterial influences
- Lipid metabolism and biosynthesis
- Machine Learning in Bioinformatics
- Immunotherapy and Immune Responses
- Vector-Borne Animal Diseases
- Complement system in diseases
- Protein purification and stability
- Psoriasis: Treatment and Pathogenesis
- Machine Learning and ELM
- Ginseng Biological Effects and Applications
- Neural Networks and Applications
- Perfectionism, Procrastination, Anxiety Studies
- Exercise and Physiological Responses
Air Force Medical University
2025
Xijing Hospital
2025
Sun Yat-sen University
2008-2024
Viva Biotech (China)
2023
National University of Singapore
2013-2020
East China University of Science and Technology
2018
Duke-NUS Medical School
2013-2015
Chinese Academy of Agricultural Sciences
2009
There are four closely-related dengue virus (DENV) serotypes. Infection with one serotype generates antibodies that may cross-react and enhance infection other serotypes in a secondary infection. We demonstrated DENV 2 (DENV2)-specific human monoclonal antibody (HMAb) 2D22 is therapeutic mouse model of antibody-enhanced severe disease. determined the cryo-electron microscopy (cryo-EM) structures HMAb complexed two different DENV2 strains. binds across viral envelope (E) proteins dimeric...
ABSTRACT Previous binding studies of antibodies that recognized a partially or fully hidden epitope suggest insect cell-derived dengue virus undergoes structural changes at an elevated temperature. This was confirmed by our cryo-electron microscopy images incubated 37°C, where viruses change their surface from smooth to rough. Here we present the structures 37°C. Image analysis showed four classes particles. The three-dimensional (3D) map one these classes, representing half imaged...
Research Article14 January 2014Open Access A potent anti-dengue human antibody preferentially recognizes the conformation of E protein monomers assembled on virus surface Guntur Fibriansah Program in Emerging Infectious Diseases, Duke–NUS Graduate Medical School, Singapore City, Centre for BioImaging Sciences, National University Singapore, Search more papers by this author Joanne L Tan Scott Smith Department Medicine, Vanderbilt University, Nashville, TN, USA The Vaccine Center, Adamberage...
Abstract Structures of flavivirus (dengue virus and Zika virus) particles are known to near-atomic resolution show detailed structure arrangement their surface proteins (E prM in immature or M mature virus). By contrast, the capsid proteins:RNA complex, which forms core particle, is poorly understood, likely due inherent dynamics. Here, we stabilize via an antibody that binds across E proteins, resulting a subnanometer within particle. Fitting protein into densities shows presence helix...
Online game addiction (OGA) is becoming a significant problem worldwide. The aim of this study was to explore the incidence OGA and roles stressful life events, avoidant coping styles (ACSs), neuroticism in OGA. A total 651 Chinese college students were selected by random cluster sampling. Subjects completed version Young's eight-item Internet Addiction Scale (CIAS), Game Cognition (OGCAS), Revised Eysenck Personality Questionnaire Short (EPQ-RSC), College-student Stress Questionnaire,...
ABSTRACT Severe dengue virus (DENV)-associated diseases can occur in patients who have preexisting DENV antibodies (Abs) through antibody-dependent enhancement (ADE) of infection. It is well established that during ADE, DENV-antibody immune complexes (ICs) infect Fcγ receptor-bearing cells and increase the systemic viral burden be measured blood. For protection against infection with serotypes 1 to 4, strongly neutralizing Abs must elicited overcome effect ADE. Clinical observations infants...
Previous flavivirus (dengue and Zika viruses) studies showed largely spherical particles either with smooth or bumpy surfaces. Here, we demonstrate have high structural plasticity by the induction of a non-spherical morphology at elevated temperatures: club-shaped particle (clubSP), which contains cylindrical tail disc-like head. Complex formation DENV ZIKV Fab C10 stabilize viruses allowing cryoEM determination to ~10 Å resolution. The caterpillar-shaped (catSP) C10:ZIKV complex shows Fabs...
The interplay between microbiota and the onset of immune-mediated diseases is increasingly coming to light. However, role tonsillar in cutaneous inflammation remains largely unknown. We aimed determine how influence skin psoriasis uncover underlying molecular mechanisms. Tonsillar samples were collected from 24 healthy controls 28 patients. Microbial community composition was analyzed using 16S rRNA sequencing metagenomic sequencing. Serum levels short-chain fatty acids (SCFAs) measured via...
Single mutations that can substantially enhance stability are highly desirable for protein engineering. However, it is generally rare this kind of mutant to emerge from directed evolution experiments. This study used computational approaches identify hotspots in a diacylglycerol-specific lipase mutagenesis with functional hotspot and sequence consensus strategies, followed by ∆∆G calculations all possible using the Rosetta ddg_monomer protocol. mutants significant changes (≤−2.5 kcal/mol)...
Data-driven machine learning is increasingly involved in human life and industrial development due to its large-scale testing low time cost. However, existing algorithms are not suitable for real-world applications with data dilemmas, such as extremely high-dimension-low-sample-size problems, non-Gaussian noise, uncertainty. In this article, we propose a novel fuzzy multikernel subspace (FMKSL) address these which provides robust representation constraint sparse coding. We then develop an...
Nanobodies have emerged as promising tools in biomedicine due to their single-chain structure and inherent stability. They generally convex paratopes, which potentially prefer different epitope sites an antigen compared traditional antibodies. In this study, a synthetic phage display nanobody library was constructed used identify nanobodies targeting tumor-associated antigen, the human B7-H3 protein. Combining next-generation sequencing single-clone validation, two were identified...
Charge heterogeneity is one of the most critical quality attributes antibodies, which has strong influence on drug’s biological activity and safety. Finding out key components that affecting charge variants great significance for establishing a competitive culture process. In this study, we first illustrated uridine’s impacts antibody in CHO cell fed-batch cultures. Uridine was beneficial to growth maintenance viability, made IVCC increased by 50% final titer improved 64%. However, uridine...
Of all the aerobic respiration by-products, cytotoxic superoxide derived from mitochondrial-leaked electrons, is only one known to be disposed of intracellularly. Is this fate destiny for electrons? When Cynomolgus monkeys were injected intravenously with reactive oxygen species (ROS) indicators, connective tissues dura mater, facial fascia, pericardium, linea alba, dorsa fascia and other body parts, emitted specific intense fluorescent signals. Moreover, signals along alba SD rats, did not...