A5103007034- Cancer Immunotherapy and Biomarkers
- Lung Cancer Treatments and Mutations
- Lung Cancer Research Studies
- Cancer Cells and Metastasis
- Pancreatic and Hepatic Oncology Research
- Cancer Genomics and Diagnostics
- Cancer-related molecular mechanisms research
- Esophageal Cancer Research and Treatment
- Colorectal and Anal Carcinomas
- Colorectal Cancer Treatments and Studies
- Ferroptosis and cancer prognosis
- Cancer, Hypoxia, and Metabolism
- Epigenetics and DNA Methylation
- Immunotherapy and Immune Responses
- Neuroendocrine Tumor Research Advances
- Colorectal Cancer Surgical Treatments
- Immune cells in cancer
- Bladder and Urothelial Cancer Treatments
- Wnt/β-catenin signaling in development and cancer
- Radiomics and Machine Learning in Medical Imaging
- Colorectal Cancer Screening and Detection
- Brain Metastases and Treatment
- Cell Adhesion Molecules Research
- NF-κB Signaling Pathways
- Cancer-related gene regulation
Chinese Academy of Sciences
2001-2023
Zhejiang Cancer Hospital
2012-2023
Huangshi Central Hospital
2023
University of Chinese Academy of Sciences
2019-2022
Cancer Hospital of Chinese Academy of Medical Sciences
2019-2022
Sichuan University
2019
Hangzhou Cancer Hospital
2019
Abstract Extracellular vesicles (EVs) are excellent potential vectors for the delivery of therapeutic drugs. However, issues with biological safety and disease targeting substantially limit their clinical application. EVs from red blood cells (RBC‐EVs) drug vehicles because unique safety. Here, we demonstrated that EVs, including RBC‐EVs, show natural liver accumulation. Mechanistically, environment induces macrophages to phagocytize RBC‐EVs in a C1q‐dependent manner. loaded antisense...
// Kaiyan Chen 1, 2, * , Guoping Cheng 3, Fanrong Zhang 1 Nan Dan Li 2 Jiaoyue Jin Junzhou Wu Lisha Ying Weimin Mao Su Cancer Research Institute, Zhejiang Hospital and Key Laboratory Diagnosis Treatment Technology on Thoracic Oncology of Province, Hangzhou, China Department Oncology, The Second Clinical Medical College Chinese University, 3 Pathology, Hospital, These authors contributed equally to this work Correspondence to: Su, email: sudan@zjcc.org.cn Mao, maowm1218@163.com Keywords:...
Programmed cell death ligand 1 (PD-L1) is a critical biomarker for predicting the response to immunotherapy. However, traditional quantitative evaluation of PD-L1 expression using immunohistochemistry staining remains challenging pathologists. Here we developed deep learning (DL)-based artificial intelligence (AI) model automatically analyze immunohistochemical in lung cancer patients. A total 1,288 patients with were included study. The diagnostic ability three different AI models (M1, M2,...
Several clinical trials have shown that immune treatment focus on programmed death-1 and death-ligand 1 (PD-L1) yields a good efficacy in advanced non-small cell lung cancer (NSCLC). We investigated whether the PD-L1 expression was related to clinicopathologic molecular characteristics patients with surgically resected NSCLC.Between December 2008 2013, formalin-fixed, paraffin-embedded samples were obtained from adenocarcinoma at Zhejiang Cancer Hospital. RT-PCR used analyze EGFR, KRAS,...
Background Insertions in exon 20 (Ex20ins) of epidermal growth factor receptor ( EGFR ) and human 2 HER2) are relatively insensitive to first‐ second‐generation EGFR‐tyrosine kinase inhibitors (TKIs) non‐small cell lung cancer (NSCLC). This study aimed investigate the immune microenvironment features efficacy PD‐1/PD‐L1 blockade NSCLC with HER2 Ex20ins. Methods Clinical characteristics, coexisting mutations, outcomes EGFR‐TKIs checkpoint were reviewed for patients mutations or . Data...
Immune checkpoint inhibitors play a vital role in triple-negative breast cancer (TNBC) immunotherapy. A recent study showed that chemokine-like factor (CKLF)-like MARVEL transmembrane domain containing 6 (CMTM6) has crucial programmed death-ligand 1 (PD-L1) stability. The aim of this was to investigate the relationship between CMTM6 and PD-L1 TNBC association with clinical characteristics.A total 143 patients, including 75 human epidermal growth receptor 2 (HER2)-driven 68 TNBC, were...
According to the latest World Health Organization (WHO) classification in 2015, invasive mucinous adenocarcinoma (IMA) is defined as a new pathological subtype of lung (LUAD). However, whether this rare pathology has any difference prognosis than conventional LUAD debatable. Our study attempted compare clinical characteristics and IMA vs. noninvasive adenocarcinomas (NMA).A total 1,857 patients with who underwent radical resection were screened from 2010 2015 at Zhejiang Cancer Hospital....
Previous small-size studies reported BRAF-mutated NSCLC patients have comparable sensitivity to immune checkpoint inhibitors (ICIs). However, how BRAF mutation affects the tumor microenvironment (TIME) is unknown. We performed Nanostring-panel RNA sequencing evaluate TIME in 57 mutated and wild-type (WT) specimens (cohort A). The efficacy of ICI monotherapy or combined therapies was determined 417 with WT B). found that BRAF-mutant tumors had similar ratios CD8+ T cells Tregs, balance...
Gastric cancer patient with ovarian metastasis is common in clinical practice, but it still uncertain whether surgical resection of could improve the outcome. This study aimed to explore survival benefit metastasectomy plus chemotherapy over alone treatment Krukenberg tumors arising from gastric and identify prognostic factors.A total 152 patients were identified, including 93 synchronous 59 metachronous metastasis. Overall (OS) was significantly better group relative non-metastasectomy for...
Abstract Previous studies from the Cancer Cell Line Encyclopedia (CCLE) project have adopted commercial pan-cancer cell line models to identify drug sensitivity biomarkers. However, biomarkers in esophageal squamous carcinoma (ESCC) not been widely explored. Here, eight patient-derived lines (PDCs) are successfully established 123 patients with ESCC. The mutation profiling of PDCs can partially recapture tumor tissue actionable mutations 161 Based on these and relative pathways PDCs, 46...
BACKGROUND The immune status within the tumor microenvironment has not been well determined in esophageal squamous cell carcinoma (ESCC). aim of this study was to investigate distributions tumor-infiltrating T lymphocytes (TILs), and analyze their associations with clinical characteristics prognosis; as expression programmed death-ligand 1 (PD-L1) which identified a favorable indicator prognosis our previous on ESCC. MATERIAL AND METHODS Five hundred thirty-six patients who underwent radical...
Tumor-infiltrating lymphocytes (TILs) play a critical role in tumor immune surveillance and suppression. Understanding infiltrating T cell subset density, location PD-1/PD-L1 expression might provide insight for the prediction of therapeutic response clinical outcome. The purpose this study was to evaluate localization CD8, FoxP3, PD-1, PD-L1 primary tissues their effects on prognosis stage IV M0 locally advanced nasopharyngeal carcinoma (NPC) patients.Sixty NPC patients with disease were...
Brain metastasis (BM) is a common malignant event in lung cancer. Here, we recruited 33 cancer patients with brain oligo-metastasis to explore the genomic features and tumor immune microenvironment (TIME) of BM independently. For profiling, targeted sequencing was performed. We found that high-frequent ZFHX3 occurred (40%) (28%), which might relate event; vast majority had lesions-shared mutations primary BM, confirming clonal events; EGFR most frequently gene both indicating its driver...
Programmed death ligand 1 (PD-L1) tumor proportion score (TPS) is currently widely used for selection of immune therapies in non-small cell lung cancer (NSCLC). Most samples PD-L1 expression were obtained from tissue. However, the feasible malignant pleural effusion (MPE) cytological detection poorly reported. And correlation between oncogene mutations and based on high-throughput sequencing rarely studied.NSCLC MPE partially paired tissue our institution analyzed immunohistochemistry (IHC)...
Background and objective: The aim of this study was to evaluate the expression cytomembranic programmed death-ligand 1 (PD-L1) its clinical significance in locoregionally advanced nasopharyngeal carcinoma (NPC). Patients methods: Formalin-fixed, paraffin-embedded tissue biopsies from 85 patients with histological diagnosis NPC treated radical intensity-modulated radiotherapy concurrent cisplatin-based chemotherapy were studied. By using immunohistochemistry staining, expressions PD-L1 on...
The present study was performed to quantify tumor neo-vessels, macrophages and fibroblasts in the microenvironment of hepatocellular carcinoma (HCC) explore prognostic factors HCC. distribution quantified by immunohistochemistry inverted microscopy with CRi Nuance multispectral imaging system, correlation these parameters clinico-pathological characteristics overall survival patients analyzed. number neo-vessels macrophages, density fibroblasts, as calculated thickness stroma...
Fas signaling-activated signal transducers and activators of transcription 3 (STAT3) is required for Fascin upregulation. As an actin-bundling protein, can mediate gastric cancer (GC) cell migration. Gastric AGS cells were treated with anti-Fas (5 μg/ml) 2 h, in order to stimulate the activation signaling. The vitro migration was assessed using Transwell chambers. levels phosphorylated STAT3 detected by Western blotting analyses. Nude mice injected intravenously or inhibitor without...
Background: Graded prognostic assessment for lung cancer using molecular markers (Lung-molGPA) brain metastases is a powerful tool. However, it has not been validated non-small-cell (NSCLC) patients with synchronous or metachronous metastases. Methods: A total of 1184 NSCLC were reviewed in this study. Comparative clinicopathological variables and survival analysis these two groups (synchronous vs metachronous), as well complimentary factors the entire patient cohort, performed. Afterward,...
Renal cell carcinoma (RCC) is the highest mortality rate of genitourinary cancers, and treatment options are very limited. Thus, identification molecular mechanisms underlying RCC tumorigenesis, critical for identifying biomarkers diagnosis prognosis.To validate whether IGF-I/JAK2-STAT3/miR-21 signaling pathway associated with human growth.qRT-PCR Western blotting were used to detect mRNA protein expression levels, respectively. The MTT assay was performed determine survival rate. Annexin...
The upregulation of discoidin domain receptor tyrosine kinase 2 (DDR2) has been reported to be associated with poor prognosis and metastasis in numerous tumor types by inducing epithelial‑mesenchymal transition (EMT); however, the expression profile DDR2 papillary thyroid carcinoma (PTC) local effect on PTC cells remain unknown. aim present study was investigate levels tissues patients cell lines determine EMT cells. In study, it demonstrated that significantly increased human lines....
Checkpoint kinase 1 (CHK1) and p53 are involved in cell‑cycle checkpoint, cellular response to DNA damage. CHK1 overexpressed bladder urothelial carcinoma (BUC); however, a clear elucidation on their interaction influence the progress of BUC is absent. The aim present study was examine correlation between BUC, analyze value as therapeutic targets prognostic indicators BUC. A clinically annotated cohort 110 patients with identified retrospectively. EnVision‑based immunohistochemistry western...