A5107677890- Reproductive Biology and Fertility
- Mesenchymal stem cell research
- Circadian rhythm and melatonin
- Pluripotent Stem Cells Research
- Renal and related cancers
- Tissue Engineering and Regenerative Medicine
- Stress Responses and Cortisol
- Genetics, Aging, and Longevity in Model Organisms
- TGF-β signaling in diseases
- Reproductive System and Pregnancy
- Developmental Biology and Gene Regulation
- Plant Molecular Biology Research
- Ovarian cancer diagnosis and treatment
- Bone Metabolism and Diseases
- Dietary Effects on Health
- Birth, Development, and Health
- Ovarian function and disorders
- Microtubule and mitosis dynamics
- Osteoarthritis Treatment and Mechanisms
- Menopause: Health Impacts and Treatments
- Endometriosis Research and Treatment
- Corneal Surgery and Treatments
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
Shanghai Jiao Tong University
2014-2025
International Peace Maternity & Child Health Hospital
2015-2025
Boston VA Research Institute
2023-2024
Harvard University
2023-2024
Shanghai Municipal People's Government
2020
Human endometrial mesenchymal stem cells (EnSCs) derived from menstrual blood have stem/stromal (MSCs) characteristics and can differentiate into cell types that arise all three germ layers. We hypothesized EnSCs may offer promise for restoration of ovarian dysfunction associated with premature failure/insufficiency (POF/POI).Mouse ovaries were injured busulfan cyclophosphamide (B/C) to create a damaged ovary mouse model. Transplanted injected the tail vein sterilized mice (Chemoablated...
Human amniotic epithelial cells (hAECs) are attractive candidates for regenerative medical therapy, with the potential to replace deficient and improve functional recovery after injury. Previous studies have demonstrated that transplantation of hAECs effectively alleviate chemotherapy-induced ovarian damage via inhibiting granulose apoptosis in animal models premature failure/insufficiency (POF/POI). However, underlying molecular mechanism accounting hAECs-mediated function is not fully...
Abstract INTRODUCTION Premature ovarian insufficiency (POI), marked by function loss before age 40, is linked to a higher risk of dementia, including Alzheimer's disease (AD). However, the associated brain structural changes remain poorly understood. METHODS We analyzed T1‐weighted and diffusion tensor imaging in 33 idiopathic POI women 51 healthy controls, using voxel‐based, surface‐based morphometry, network analyses assess gray matter volume (GMV), cortical thickness, connectivity....
Skin-derived mesenchymal stem cells (SMSCs) can differentiate into the three embryonic germ layers. For this reason, they are considered a powerful tool for therapeutic cloning and offer new possibilities tissue therapy. Recent studies showed that skin-derived expressing germ-cell specific markers in vitro form oocytes vivo. The idea SMSCs may be suitable treatment of intractable diseases or traumatic damage has attracted attention. To determine ability to reactivate injured ovaries, mouse...
Human amnion epithelial cells (hAECs) transplantation via tail vein has been reported to rescue ovarian function in mice with chemotherapy-induced primary insufficiency (POI). To test whether intraperitoneally transplanted hAECs could induce therapeutic effect and characterize the paracrine of hAECs, we utilized a chemotherapy induced model POI investigated ability conditioned medium collected from cultured (hAECs-CM) restore function. We found that or hAECs-CM either 24 hours 7 days after...
Chronic stress is an important factor influencing people's health. It usually causes endocrinal disorders and a decline in reproduction females. Although studies of both human animals suggest detrimental effect on reproduction, the influence chronic ovarian reservation follicular development still not clear. In this study, restraint (CRS) mouse model was used to investigate explore underlying mechanism. after 8 weeks CRS, primordial follicles were excessively activated ovaries CRS group...
Chronic stress which is common in the current society can be harmful to female reproduction and associated with oocyte defects. However, underlying mechanisms remain largely unknown. Herein, by using a mouse model of chronic restraint stress, we demonstrated that could induce meiotic spindle abnormalities, chromatin misalignment, mitochondrial dysfunction elevated ROS levels oocytes vivo, all were normalized administration melatonin. Consistently, melatonin treatment during vitro maturation...
Follicular fluid is important for follicular development and oocyte maturation. Evidence suggests that not only rich in proteins but cells. Besides oocytes, the contains granulosa, thecal, ovarian surface epithelial cells, both granulosa thecal cells are well-characterized. However, poorly studied. This study aims to isolate characterize vitro originate from human retrieved assisted fertilization program.Follicular samples were collected 20 women reproduction program. Epithelial...
The Forkhead box transcription factors FOXC1 and FOXC2 are expressed in condensing mesenchyme cells at the onset of endochondral ossification. We used Prx1-cre mouse to ablate Foxc1 Foxc2 limb skeletal progenitor cells. Prx1-cre;Foxc1Δ/Δ;Foxc2Δ/Δ limbs were shorter than controls, with worsening phenotypes distal structures. Cartilage formation mineralization was severely disrupted paws. radius tibia malformed, whereas fibula ulna remained unmineralized. Chondrocyte maturation delayed, fewer...
Psychological stress, which exerts detrimental effects on human reproduction, may compromise the meiotic competence of oocytes. Meiotic resumption, germinal vesicle breakdown (GVBD), is first milestone to confer In practice assisted reproductive technology (ART), timing for GVBD associated with rates cleavage and blastocyst formation. However, whether chronic stress compromises oocyte by influencing underlying mechanisms are unclear. present study, a restraint (CRS) mouse model was used...
Abstract Sleep deprivation (SD) is observed to adversely affect the reproductive health of women. However, its precise physiological mechanisms remain largely elusive. In this study, using a mouse model SD, it demonstrated that SD induces depletion ovarian primordial follicles, phenomenon not attributed immune‐mediated attacks or sympathetic nervous system activation. Rather, excessive secretion stress hormones, namely norepinephrine (NE) and epinephrine (E), by overactive adrenal glands,...
The forkhead box transcription factor genes Foxc1 and Foxc2 are expressed in the condensing mesenchyme of developing skeleton prior to onset chondrocyte differentiation. To determine roles these factors limb development we deleted both lateral plate mesoderm using Prx1-cre mouse line. Resulting compound homozygous mice died shortly after birth with exencephaly, malformations this sternum skeleton. Notably distal structures were preferentially affected, autopods displaying reduced or absent...