A5101558565- Parasitic Infections and Diagnostics
- Endometriosis Research and Treatment
- Reproductive System and Pregnancy
- Cervical Cancer and HPV Research
- Congenital Heart Disease Studies
- MicroRNA in disease regulation
- Plant Virus Research Studies
- Insect and Pesticide Research
- Gynecological conditions and treatments
- Electrospun Nanofibers in Biomedical Applications
- Kawasaki Disease and Coronary Complications
- Coronary Artery Anomalies
- Cardiac Valve Diseases and Treatments
- Autophagy in Disease and Therapy
- Urological Disorders and Treatments
- interferon and immune responses
- Abdominal Trauma and Injuries
- Tissue Engineering and Regenerative Medicine
- NF-κB Signaling Pathways
- Reproductive Biology and Fertility
- Immune Cell Function and Interaction
- Cardiovascular and Diving-Related Complications
- Cancer-related molecular mechanisms research
- Ovarian function and disorders
- Cancer, Hypoxia, and Metabolism
Southwest University
2018-2025
Obstetrics and Gynecology Hospital of Fudan University
2021-2024
Shenzhen Children's Hospital
2010-2024
University Medical Center
2024
University Hospital and Clinics
2024
Monmouth Medical Center
2023
State Key Laboratory of Silkworm Genomic Biology
2018-2022
International Peace Maternity & Child Health Hospital
2018-2022
Shanghai Jiao Tong University
2018-2022
University of Toronto
2022
Intrauterine adhesion (IUA) is an of the uterine cavity or cervical canal resulting from damage to basal layer endometrium; this condition usually accompanied by fibrosis endometrium. Previous studies have demonstrated that human amniotic epithelial cells (hAECs) stem cell characteristics; however, it unclear whether hAECs therapeutic potential restore fertility after IUA.A murine IUA model was established mechanical injury uterus. Then, 106 were transplanted intraperitoneal injection. The...
Premature ovarian failure (POF) is one of the most common complications among female patients with tumors treated chemotherapy and requires advanced treatment strategies. Human amniotic epithelial cell (hAEC)-based therapy mediates tissue regeneration in a variety diseases, increasing evidence suggests that therapeutic efficacy hAECs mainly depends on paracrine action. This study aimed to identify exosomes derived from explored potential ovaries damaged by underlying molecular mechanism....
The microsporidian Nosema bombycis is an obligate intracellular parasite of Bombyx mori , that lost its intact tricarboxylic acid cycle and mitochondria during evolution but retained glycolysis pathway. N. hexokinase (NbHK) not only a rate-limiting enzyme also secretory protein. Indirect immunofluorescence assays recombinant HK overexpressed in BmN cells showed NbHK localized the nucleus cytoplasm host cell meront stage. When matured, tended to concentrate at nuclei cells. Furthermore,...
Nosema bombycis is a destructive, obligate intracellular parasite of the Bombyx mori. In this study, single-chain variable fragment (scFv) dependent technology developed for purpose inhibiting proliferation in insect cells. The scFv-G4, which we prepared from mouse G4 monoclonal antibody, can target N. spore wall protein 12 (NbSWP12). Indirect immunofluorescence assays showed that NbSWP12 located mainly on outside cytoskeleton, although some it co-localized with β-tubulin meront-stage...
Epithelial ovarian cancer (EOC) is the most fatal gynecologic malignancy due to its late diagnosis and lack of curative therapy. The antimalaria compound artemisinin derivatives, such as artesunate (ART) dihydroartemisinin (DHA), have proven be potent anticancer drugs act through various mechanisms. To identify novel targets derivatives in EOC cells, we investigated effects ART DHA on SKOV3 primary cell growth via CCK-8 assay. Both inhibited growth. A cycle distribution analysis showed that...
Epithelial ovarian cancer has the highest mortality rate of all gynecologic cancers. Cancer stem cells are considered to be initiating tumors. It is known that spheroid culture promotes acquire cell characteristics and become cell‐like. But mechanisms remain largely unclear. Our data show autophagy sustainably activated in cells. Inhibition by knockdown ATG5 abolishes self‐renewal ability Knockdown prevents enter quiescent state. Autophagy critical for reenter cycle because rapamycin can...
Chronic stress which is common in the current society can be harmful to female reproduction and associated with oocyte defects. However, underlying mechanisms remain largely unknown. Herein, by using a mouse model of chronic restraint stress, we demonstrated that could induce meiotic spindle abnormalities, chromatin misalignment, mitochondrial dysfunction elevated ROS levels oocytes vivo, all were normalized administration melatonin. Consistently, melatonin treatment during vitro maturation...
Microsporidia are a group of intracellular pathogens that actively manipulate host cell biological processes to facilitate their niche. Apoptosis is an important defense mechanism by which control pathogens. modulating apoptosis has been reported previously, however the molecular not yet clear. In this report, we describe microsporidia Nosema bombycis inhibits Bombyx mori cells through secreted protein NbSPN14, serine protease inhibitor (Serpin). An immunofluorescent assay demonstrated upon...
Background and Purpose Primary ovarian insufficiency (POI) has serious physical psychological consequences due to estradiol deprivation, leading increased morbidity mortality. However, the causes of most POI cases remain unknown. Psychological stress, usually caused by stressful life events, is known be negatively associated with function. It important explore high-frequency adverse events among women for future interventions. Methods Forty-three (mean age=33·8 years) were recruited who...
Abstract Persistent high‐risk human papilloma virus (HR‐HPV) infection is the main risk factor for cervical cancer, threatening women's health. Despite growing prophylactic vaccination, annual cancer cases are still increasing and show a trend of younger onset age. However, therapeutic approaches towards HPV limited. 25‐hydrocholesterol (25HC) has wide‐spectrum inhibitory effect on variety viruses. To explore efficient interventions to restrict at an early time, we applied different...
Psychological stress, which exerts detrimental effects on human reproduction, may compromise the meiotic competence of oocytes. Meiotic resumption, germinal vesicle breakdown (GVBD), is first milestone to confer In practice assisted reproductive technology (ART), timing for GVBD associated with rates cleavage and blastocyst formation. However, whether chronic stress compromises oocyte by influencing underlying mechanisms are unclear. present study, a restraint (CRS) mouse model was used...
Abstract Objectives During the winter outbreak of 2009 influenza A (H1N1) infection in China, number confirmed cases and fatal has grown rapidly. We describe clinical characteristics hospitalized children with Shenzhen, November–December 2009. Methods Using a standardized form, we collected data on 148 children. was nasopharyngeal swab specimens use real‐time reverse transcriptase‐polymerase chain reaction assay. Results Of infection, 81 (55%) were 5 years age or older 85% patients...
Microsporidia are ubiquitous in the environment, infecting almost all invertebrates, vertebrates, and some protists. The microsporidian Nosema bombycis causes silkworms pébrine disease leads to huge economic losses. Parasite secreted proteins play vital roles pathogen-host interactions. Serine protease inhibitors (serpins), belonging largest most broadly distributed inhibitor superfamily, also found Microsporidia. In this study, we characterized 19 serpins (NbSPNs) N. bombycis; eight of them...
Single-particle aerosol mass spectrometry is a practical method for studying microbial aerosols. However, the related spectral characteristics of single-cell microorganisms have not yet been studied systematically; hence, further investigations are necessary.Different cells were grown and directly aerosolized in laboratory. These aerosols then drawn into single-particle spectrometer platform, spectra profiles obtained real-time. The biological characteristics, ion variation trends, types...
Abstract Background We aimed to examine predictive measures for medium and giant coronary artery aneurysms (CAA) in Kawasaki disease (KD) patients. Methods Patients who were diagnosed with KD from 2015 2021 retrospectively reviewed. The clinical laboratory data compared between medium-giant group non-medium-giant group. Results A total of 1331 patients investigated, whom 63 (4.7%) developed CAA including 27 (2%) CAA. Sex, age, fever duration, intravenous immunoglobulin (IVIG) resistance,...
Abstract Background Patients with Turner syndrome (TS) face an increased risk of developing aortic dilatation (AD), but diagnosing AD in children presents greater complexity compared to adults. This study aimed investigate the application various assessment indicators Chinese and adolescents TS. Methods included TS patients admitted Shenzhen Children’s Hospital from 2017 2022. Cardiovascular lesions were diagnosed by experienced radiologists. without structural heart disease divided into...
An ectopic kidney is a rare congenital defect that often asymptomatic, but can be incidentally discovered during imaging examinations. Moreover, the morphological characteristics and laboratory features of kidneys are nonspecific, which may lead to misleading diagnostic approaches, particularly when there additional factors, such as infection, obstruction, or other anomalies. A 43-year-old female presented with mass in left adnexal area. She had septate uterus history urinary incontinence....
Epithelial ovarian cancer (EOC) is the most lethal of all gynecologic tumors. Cancer spheroid culture a widely used model to study stem cells. Previous studies have demonstrated effectiveness cytokine‑induced killer (CIK) cell‑based therapies against and However, it not clear how EOC cells respond CIK‑mediated cellular lysis, mechanisms involved never been reported before. A flow cytometry‑based method was evaluate anti‑cancer effects CIK adherent A2780 spheroids. To demonstrate association...
We reported the 3-year follow-up results of initial clinical experience with AbsnowTM device, a novel biodegradable occluder for percutaneous closure atrial septal defect (ASD).The device is total double-disc poly-L-lactic acid (PLLA) framework and PLLA membranes intergraded into to ensure its biodegradability, safety, efficacy.Five pediatric patients were enrolled from May June 2018 in our institution followed up 3 years. A evaluation transthoracic echocardiography performed at 24 hr, 1...
Intrauterine adhesion (IUA), often leading to gynecological complications including amenorrhea, abdominal pain and infertility, is frequently induced by injuries the endometrium. Hence it would be of great benefit take efforts prevent after intrauterine operations. Orally administration 17β-estradiol (E2) commonly used promote endometrium regeneration, but limited low concentrations at injured sites. We aim preparing an E2-releasing uterine stent, which could improve efficiency E2 therapy...
Purpose: We aim to identify risk factors associated with pathological upgrading/downgrading after conization in patients cervical high-grade squamous intraepithelial lesion and provide stratification management based on machine learning predictive model. Methods: 1. This retrospective study included who visited Obstetrics Gynecology Hospital of Fudan University from January 1 December 31, 2019, were diagnosed (HSIL) by colposcopy directed biopsy (CDB) subsequently underwent conization. A...