A5100391152- Autophagy in Disease and Therapy
- Reproductive Biology and Fertility
- Iron Metabolism and Disorders
- Hemoglobinopathies and Related Disorders
- Endoplasmic Reticulum Stress and Disease
- Nanoplatforms for cancer theranostics
- Epigenetics and DNA Methylation
- Photodynamic Therapy Research Studies
- MicroRNA in disease regulation
- Circadian rhythm and melatonin
- Mesenchymal stem cell research
- Trace Elements in Health
- RNA modifications and cancer
- Cannabis and Cannabinoid Research
- Renal and related cancers
- Neuroinflammation and Neurodegeneration Mechanisms
- Prostate Cancer Treatment and Research
- Toxoplasma gondii Research Studies
- Pancreatic function and diabetes
- Assisted Reproductive Technology and Twin Pregnancy
- Healthcare and Venom Research
- Pluripotent Stem Cells Research
- Endometriosis Research and Treatment
- Flavonoids in Medical Research
- Calcium signaling and nucleotide metabolism
Shanghai Jiao Tong University
2015-2025
Shanxi Medical University
2021-2025
International Peace Maternity & Child Health Hospital
2015-2025
Zhengzhou University
2016-2024
Henan Provincial People's Hospital
2016-2024
Zhongnan Hospital of Wuhan University
2021-2024
Wuhan University
2021-2024
Southwest Hospital
2022-2024
Army Medical University
2022-2024
Tang Hospital
2024
Decline in semen quality has become a global public health concern. Psychological stress is common the current modern society and associated with decline. Increasing evidence demonstrated that melatonin anti-apoptotic antioxidant functions. Whether can ameliorate damage testes induced by psychological never been investigated. Here, mouse model of restraint normalized sperm density decline, testicular cells apoptosis, oxidative stressed male mice. Melatonin decreased reactive oxygen species...
Autophagy is a catabolic lysosomal degradation process essential for cellular homeostasis and cell survival. Dysfunctional autophagy has been associated with wide range of human diseases, e.g., cancer neurodegenerative diseases. A large number small molecules that modulate have widely used to dissect this some them, chloroquine (CQ), might be ultimately applied treat variety autophagy-associated Here we found vacuolin-1 potently reversibly inhibited the fusion between autophagosomes...
Reactive oxygen species (ROS) have been commonly accepted as inducers of autophagy, and autophagy in turn is activated to relieve oxidative stress. Yet, whether how stress, generated various human pathologies, regulates remains unknown. Here, we mechanistically studied the role TRPM2 (transient receptor potential cation channel subfamily M member 2)-mediated Ca2+ influx stress-mediated regulation. On one hand, demonstrated that stress triggered TRPM2-dependent inhibit induction early which...
Premature ovarian failure and insufficiency are significant long-term side-effects of chemotherapy for female cancer patients. Recently, stem cell transplantation has been identified as a promising treatment premature insufficiency. We have previously demonstrated that human amniotic epithelial cells (hAECs) migrate into injured tissue promote the recovery function in chemoablated mice. However, molecular mechanism guiding this process remains unclear.To further investigate effect hAECs on...
Abstract Afatinib, a second-generation tyrosine kinase inhibitor (TKI), has been approved for the treatment of advanced EGFR -mutant non-small cell lung cancer (NSCLC). However, afatinib’s clinical application is still hampered by acquired resistance. Recently, autophagy considered as an important mechanism resistance to TKI. Herein, we investigated induction well its influence on anti-lung adenocarcinoma activity afatinib in two activating -mutants H1975 and H1650 cells. First, Growth...
RNA-Seq and gene set enrichment anylysis revealed that ovarian cancer associated fibroblasts (CAFs) are mitotically active compared with normal (NFs). Cellular senescence is observed in CAFs treated H2O2 as shown by elevated SA-β-gal activity p21 (WAF1/Cip1) protein levels. Reactive oxygen species (ROS) production elevation may account for H2O2-induced cell cycle arrest S phase. Blockage of autophagy can increase ROS CAFs, leading to phase, proliferation inhibition enhanced sensitivity...
Abstract Artesunate (ART) has been indicated as a candidate drug for hepatocellular carcinoma (HCC). Glucosylceramidase (GBA) is required autophagic degradation. Whether ART regulates flux by targeting GBA in HCC remains to be defined. Herein, our data demonstrated that the dramatic overexpression of was significantly associated with aggressive progression and short overall survival times HCC. Subsequent experiments revealed an association between activity expression clinical samples, tumor...
Chronic stress has been shown to facilitate progression of epithelial ovarian cancer (EOC), however, the neuro-endocranial mechanism participating in this process still remains unclear. Here, we reported that chronic restraint (CRS) promoted abdominal implantation metastasis EOC cells and expression epithelial-mesenchymal transition-related markers tumor-bearing mouse model, including TWIST, SLUG, SNAIL, β-catenin. We observed β-catenin co-expressed with SLUG norepinephrine (NE) tumor...
To determine the role of autophagy in innate immune response to fungal keratitis (FK) caused by Aspergillus fumigatus infection.Corneal samples obtained from patients and mice with FK were visualized via transmission electron microscopy (TEM). Autophagy-related proteins LC3B-II, Beclin-1, LAMP-1, p62 A. fumigatus-infected corneas C57BL/6 tested Western blot. After treatment inhibitors 3-methyladenine (3-MA), chloroquine (CQ), or inducer rapamycin, autophagy-related detected Corneas...
Rationale: Impairment of autophagy maturation has been implicated in Alzheimer's disease (AD) pathogenesis.However, the mechanism for this impairment not elucidated, and whether enhancing is a viable therapeutic strategy AD verified.Methods: We examined autophagosome process cell mouse models by immunoblotting.To further understand changes brains, we analyzed transcriptome RNA-sequencing measured expression RAB7, CCZ1 MON1A.We performed brain stereotaxic injections AAV into 3xTg WT to...
Abstract Background Anemia is a common complication of HIV/AIDS, particularly in adolescents and young adults across various countries regions. However, little known about the changing prevalence trends anemia impairment this population over time. Methods Data on with HIV/AIDS from 1990 to 2019 were collected Global Burden Disease. Prevalence was calculated by gender, region, country for individuals aged 10–24, measured using estimating annual percentage changes (EAPC). Results Globally,...
Abstract Pax4 ‐deficient mice have a severe gastrointestinal endocrine deficiency: they lack most pancreatic cells that produce insulin or somatostatin and various duodenal cell types. Remarkably, also an overabundance of ghrelin‐expressing in the pancreas duodenum. Detailed analysis nullizygous determined mutant islets are largely composed distinctive type expresses ghrelin, glucagon, islet amyloid polypeptide (IAPP), low levels Pdx1. Lineage‐tracing revealed these unique directly arose...
Human amnion epithelial cells (hAECs) transplantation via tail vein has been reported to rescue ovarian function in mice with chemotherapy-induced primary insufficiency (POI). To test whether intraperitoneally transplanted hAECs could induce therapeutic effect and characterize the paracrine of hAECs, we utilized a chemotherapy induced model POI investigated ability conditioned medium collected from cultured (hAECs-CM) restore function. We found that or hAECs-CM either 24 hours 7 days after...
Breast cancer is a common malignancy threatening women’s health around the world. Despite improved treatments for different subtypes of breast tumors that have been put forward, there still exists poor therapeutic response and prognosis. Magnetic fields, as non-invasive therapy, shown anti-tumor effects in vitro vivo; however, detailed mechanisms involved are not clear. In this study, we found exposure to low-frequency magnetic fields (LF-MFs) with an intensity 1 mT frequencies 50, 125, 200,...
Objectives: Ovarian cancer is a common gynaecological malignancy. Photodynamic therapy (PDT) mediated by 5-aminolaevulinic acid (5-ALA-PDT) widely used in clinical practice. However, hypoxia may impact the efficacy of this treatment. In present study, we combined bioreductively active drug tirapazamine (TPZ) with PDT to explore its potential enhancing ovarian cell death. Methods: A counting kit-8 assay was determine cytotoxicity under different intervention conditions. The distribution...
Epithelial ovarian cancer has the highest mortality rate of all gynecologic cancers. Cancer stem cells are considered to be initiating tumors. It is known that spheroid culture promotes acquire cell characteristics and become cell‐like. But mechanisms remain largely unclear. Our data show autophagy sustainably activated in cells. Inhibition by knockdown ATG5 abolishes self‐renewal ability Knockdown prevents enter quiescent state. Autophagy critical for reenter cycle because rapamycin can...
Chronic stress is an important factor influencing people's health. It usually causes endocrinal disorders and a decline in reproduction females. Although studies of both human animals suggest detrimental effect on reproduction, the influence chronic ovarian reservation follicular development still not clear. In this study, restraint (CRS) mouse model was used to investigate explore underlying mechanism. after 8 weeks CRS, primordial follicles were excessively activated ovaries CRS group...
Alzheimer's disease (AD) is a chronic neurodegenerative disorder characterized by progressive cognitive impairments. Vitegnoside flavonoid present in the medicinal plant Vitex negundo, widely used as folk medicine several Asian countries including China. It possesses biological ac tivities, axon outgrowth, but no evidence available on its effect AD. Since effective treatment to cure AD, of vitegnoside this was investigated. The human neuroblastoma SH-SY5Y cell line carrying Swedish mutation...